Construct: ORF TRCN0000472834
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012296.1_s317c1
- Derived from:
- ccsbBroadEn_13206
- DNA Barcode:
- ATGAATGGGCAGACCCTTGAGTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LINC00266-1 (140849)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472834
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 140849 | LINC00266-1 | long intergenic non-protein... | NR_040415.1 | 29.4% | 1_377del;591_723del | |
| 2 | human | 441123 | LINC00266-3 | long intergenic non-protein... | NR_109817.1 | 15.5% | 1_353del;383T>C;442_443ins124 | |
| 3 | human | 728323 | LINC01881 | long intergenic non-protein... | NR_130700.1 | 13.4% | (many diffs) | |
| 4 | human | 728323 | LINC01881 | long intergenic non-protein... | NR_130699.1 | 12.7% | (many diffs) | |
| 5 | human | 728323 | LINC01881 | long intergenic non-protein... | NR_130701.1 | 12% | (many diffs) | |
| 6 | human | 346288 | SEPTIN14 | septin 14 | NM_207366.3 | 10.8% | 10.3% | (many diffs) |
| 7 | human | 346288 | SEPTIN14 | septin 14 | XM_011515373.2 | 10.8% | 10.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 250
- ORF end:
- 463
- ORF length:
- 213
- Sequence:
-
1 GGAGTACGTC GTCTTTAGGT TGGGGGGAGG GGTTTTATGC GATGGAGTTT CCCCACACTG 61 AGTGGGTGGA GACTGAAGTT AGGCCAGCTT GGCACTTGAT GTAATTCTCC TTGGAATTTG 121 CCCTTTTTGA GTTTGGATCT TGGTTCATTC TCAAGCCTCA GACAGTGGTT CAAAGTTTTT 181 TTCTTCCATT TCAGGTGTCG TGAGGCTAGC ATCGATTGAT CAACAAGTTT GTACAAAAAA 241 GTTGGCACCA TGATTCAACA GGAGGAGATA AGGAAGCTCG AGGAAGAGAA AAAACAACTG 301 GAAGGAGAAA TCATAGATTT TTATAAAATG AAAGCTGCCT CTGAAGCACT GCAGACTCAG 361 CTGAGCACTG ATACAAAGAA AGACAAACAT CCTGATCCAT ATGAATTCCT CTTATTAAGA 421 AAAATAAAGC ATCCAGGATT CAATGAAGAA CTATCACCTT GTTTGCCAAC TTTCTTGTAC 481 AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG 541 TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA 601 AAGGACGAAT GAATGGGCAG ACCCTTGAGT TGACGCGTTA AGTCgacaat caacctctgg 661 attacaaaat ttgtgaaaga tt