Construct: ORF TRCN0000472848
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013843.1_s317c1
- Derived from:
- ccsbBroadEn_04720
- DNA Barcode:
- GGGAGTGAGAAAGGGTCCACACCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TADA1 (117143)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472848
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 117143 | TADA1 | transcriptional adaptor 1 | NM_053053.4 | 100% | 100% | |
| 2 | mouse | 27878 | Tada1 | transcriptional adaptor 1 | NM_030245.3 | 89.1% | 95.8% | (many diffs) |
| 3 | mouse | 27878 | Tada1 | transcriptional adaptor 1 | XM_017321085.1 | 50.4% | 54.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1071
- ORF length:
- 1005
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gacctttgtg agcgagctgg aggcggccaa gaagaactta agcgaggccc 121 tgggggacaa cgtgaaacaa tactgggcta acctaaagct gtggttcaag cagaagatca 181 gcaaagagga gtttgacctt gaagctcata gacttctcac acaggataat gtccattctc 241 acaatgattt cctcctggcc attctcacgc gttgtcagat tttggtttct acaccagatg 301 gtgctggatc tttgccttgg ccagggggtt ccgcagcaaa acctggaaaa cccaagggaa 361 agaaaaagct ttcttctgtt cgtcagaaat ttgatcatag attccagcct caaaatcctc 421 tctcaggagc ccagcaattt gtggcaaagg atccccaaga tgatgacgac ttgaaacttt 481 gttcccacac aatgatgctt cccactcgag gccagcttga agggagaatg atagtgactg 541 cttatgagca tgggctggac aatgtcaccg aggaggctgt ttcagctgtt gtctatgctg 601 tggagaatca ccttaaagat atactgacgt cagttgtgtc aagaaggaaa gcttatcggt 661 tacgagatgg tcattttaaa tatgcctttg gcagtaacgt GACCCCGCAG CCATACCTGA 721 AGAATAGTGT AGTAGCTTAC AACAACTTAA TAGAAAGCCC TCCAGCTTTT ACTGCTCCCT 781 GTGCTGGTCA GAATCCAGCT TCTCACCCAC CCCCTGATGA TGCTGAGCAG CAGGCTGCAC 841 TCCTGCTGGC ATGCTCCGGA GACACTCTAC CTGCATCTTT GCCTCCGGTG AACATGTACG 901 ATCTTTTTGA AGCTTTGCAG GTGCACAGGG AAGTCATCCC TACACATACT GTCTATGCTC 961 TTAACATTGA AAGGATCATC ACGAAACTCT GGCATCCAAA TCATGAAGAG CTGCAGCAAG 1021 ACAAAGTTCA CCGCCAGCGC TTGGCAGCCA AGGAGGGGCT TTTGCTGTGC TACCCAACTT 1081 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1141 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1201 CTTGTGGAAA GGACGAGGGA GTGAGAAAGG GTCCACACCA ACGCGTTAAG TCgacaatca 1261 acctctggat tacaaaattt gtgaaagatt