Construct: ORF TRCN0000472900
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002005.1_s317c1
- Derived from:
- ccsbBroadEn_14683
- DNA Barcode:
- GAACGCGAACACTTGGCGACCTGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KCNA3 (3738)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472900
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3738 | KCNA3 | potassium voltage-gated cha... | NM_002232.5 | 90.8% | 90.9% | 1_156del;1035C>A |
| 2 | mouse | 16491 | Kcna3 | potassium voltage-gated cha... | NM_008418.2 | 89.9% | 96.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1635
- ORF length:
- 1569
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac cgtggtgccc ggggaccacc tgctggagcc ggaggtggcc gatggtggag 121 gggccccgcc tcaaggcggc tgtggcggcg gcggctgcga ccgctacgag ccgctgccgc 181 cctcactgcc ggccgcgggc gagcaggact gctgcgggga gcgcgtggtc atcaacatct 241 ccgggctgcg cttcgagacg cagctgaaga ccctttgcca gttccccgag acgctgctgg 301 gcgaccccaa gcggcgcatg aggtacttcg acccgctccg caacgagtac ttcttcgacc 361 gcaaccggcc cagcttcgac gccatcctct actactatca gtccgggggc cgcatccgcc 421 ggccggtcaa cgtgcccatc gacattttct ccgaggagat ccgcttctac cagctgggcg 481 aggaggccat ggagaagttc cgcgaggacg agggcttcct gcgggaggag gagcggccct 541 tgccccgccg cgacttccag cgccaggtgt ggctgctctt cgagtacccc gagagctccg 601 ggccggcccg gggcatcgcc atcgtgtccg tgctggtcat cctcatctcc attgtcatct 661 tctgcctgga gacgctgccg gagttccgcg acgagaagga ctaccccgcc tcgacgtcgc 721 aggactcatt cgaagcagcc ggcaacagca cgtcggggtc ccgcgcagga gcctccagct 781 tctccgatcc cttcttcgtg gtggagacgc tgtgcatcat ctggttctcc ttcgaactgc 841 tggtgcggtt cttcgcttgt cctagcaaag ccaccttctc gcgaaacatc atgaacctga 901 tcgacattgt ggccatcatt ccttatttta tcactctggg tacagagctg gccgaacgac 961 agggcaatgg acagcaggcc atgtctctgg ccatcctgag ggtcatccgc ctggtaaggg 1021 tcttccgcat cttcaagctg tcgcgccact ccaaggggct gcagatcctc gggcaaacgc 1081 tgaaggcgtc catgcgggag ctgggattgc tcatcttctt cctctttatt ggggtcatcc 1141 ttttctccag cgcgGTCTAC TTTGCCGAGG CAGACGACCC CACTTCAGGT TTCAGCAGCA 1201 TCCCGGATGC CTTCTGGTGG GCAGTGGTAA CCATGACAAC AGTGGGTTAC GGCGATATGC 1261 ACCCAGTGAC CATAGGGGGC AAGATTGTGG GATCTCTCTG TGCCATCGCC GGTGTCTTGA 1321 CCATCGCATT GCCAGTTCCC GTGATTGTTT CCAACTTCAA TTACTTCTAC CACCGGGAGA 1381 CAGAAGGGGA AGAGCAATCC CAGTACATGC ACGTGGGAAG TTGCCAGCAC CTCTCCTCTT 1441 CAGCCGAGGA GCTCCGAAAA GCAAGGAGTA ACTCGACTCT GAGTAAGTCG GAGTATATGG 1501 TGATCGAAGA GGGGGGTATG AACCATAGCG CTTTCCCCCA GACCCCTTTC AAAACGGGCA 1561 ATTCCACTGC CACCTGCACC ACGAACAATA ATCCCAACTC TTGTGTCAAC ATCAAAAAGA 1621 TATTCACCGA TGTTTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1681 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1741 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GAACGCGAAC ACTTGGCGAC 1801 CTGGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt