Construct: ORF TRCN0000472901

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013003.1_s317c1
Derived from:
ccsbBroadEn_01266
DNA Barcode:
TCACCCGCGCCAACGTTTCGGTAA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PPP3CA (5530)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000472901
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5530 PPP3CA protein phosphatase 3 catal... NM_001130691.2 100% 100%
2 human 5530 PPP3CA protein phosphatase 3 catal... NM_000944.5 98% 98% 1339_1368del
3 human 5530 PPP3CA protein phosphatase 3 catal... XM_017008365.1 95.3% 94.4% (many diffs)
4 human 5530 PPP3CA protein phosphatase 3 catal... NM_001130692.2 91.7% 91.7% 951_952ins126
5 human 5530 PPP3CA protein phosphatase 3 catal... XM_024454127.1 88.4% 88.4% 0_1ins150;1189_1218del
6 mouse 19055 Ppp3ca protein phosphatase 3, cata... NM_001293622.1 92.5% 99.6% (many diffs)
7 mouse 19055 Ppp3ca protein phosphatase 3, cata... NM_008913.5 90.8% 97.6% (many diffs)
8 mouse 19055 Ppp3ca protein phosphatase 3, cata... XM_017319493.2 83.3% 89.8% (many diffs)
9 mouse 19055 Ppp3ca protein phosphatase 3, cata... XM_030252464.1 83.3% 89.8% (many diffs)
10 mouse 19055 Ppp3ca protein phosphatase 3, cata... XM_017319492.2 81.8% 88% (many diffs)
11 mouse 19055 Ppp3ca protein phosphatase 3, cata... XM_030252462.1 81.8% 88% (many diffs)
12 mouse 19055 Ppp3ca protein phosphatase 3, cata... XM_030252463.1 81.8% 88% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1599
ORF length:
1533
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaagaag
61ttggcatgtc cgagcccaag gcaattgatc ccaagttgtc gacgaccgac agggtggtga
121aagctgttcc atttcctcca agtcaccggc ttacagcaaa agaagtgttt gataatgatg
181gaaaacctcg tgtggatatc ttaaaggcgc atcttatgaa ggagggaagg ctggaagaga
241gtgttgcatt gagaataata acagagggtg catcaattct tcgacaggaa aaaaatttgc
301tggatattga tgcgccagtc actgtttgtg gggacattca tggacaattc tttgatttga
361tgaagctctt tgaagtcggg ggatctcctg ccaacactcg ctacctcttc ttaggggact
421atgttgacag agggtacttc agtattgaat gtgtgctgta tttgtgggcc ttgaaaattc
481tctaccccaa aacactgttt ttacttcgtg gaaatcatga atgtagacat ctaacagagt
541atttcacatt taaacaagaa tgtaaaataa agtattcaga acgcgtatat gatgcctgta
601tggatgcctt tgactgcctt cccctggctg ccctgatgaa ccaacagttc ctgtgtgtgc
661atggtggttt gtctccagag attaacactt tagatgatat cagaaaatta gaccgattca
721aagaaccacc tgcatatgga cctatgtgtg atatcctgtg gtcagacccc ctggaagatt
781ttggaaatga gaagactcag gaacatttca ctcacaacac agtcaggggg tgttcatact
841tctacagtta cccggctgta tgtgaattct tacagcacaa taacttgtta tctatactcc
901gagcccacga agcccaagat gcagggtacc gcatgtacag gaaaagccaa acaacaggct
961tcccttctct aattacaatt ttttcagcac caaattactt agatgtatac aataacaaag
1021ctgcagtatt gaagtatgag aacaatgtta tgaatatcag gcaattcaac tgttctcctc
1081atccatactg gcttccaaat ttcatggatg tttttacttg gTCCCTTCCA TTTGTTGGGG
1141AAAAAGTGAC TGAGATGCTG GTAAATGTCC TCAACATCTG CTCAGATGAT GAACTAGGGT
1201CAGAAGAAGA TGGATTTGAT GGTGCAACAG CTGCAGCCCG GAAAGAGGTG ATAAGGAACA
1261AGATCCGAGC AATAGGCAAA ATGGCCAGAG TGTTCTCAGT GCTCAGAGAA GAGAGTGAGA
1321GTGTGCTGAC GCTGAAAGGC TTGACCCCAA CTGGCATGCT CCCCAGCGGA GTACTTTCTG
1381GAGGGAAGCA AACCCTGCAA AGCGCTATCA AAGGATTTTC ACCACAACAT AAGATCACTA
1441GCTTCGAGGA AGCCAAGGGC TTAGACCGAA TTAATGAGAG GATGCCGCCT CGCAGAGATG
1501CCATGCCCTC TGACGCCAAC CTTAACTCCA TCAACAAGGC TCTCACCTCA GAGACTAACG
1561GCACGGACAG CAATGGCAGT AATAGCAGCA ATATTCAGTG CCCAACTTTC TTGTACAAAG
1621TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT
1681GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG
1741ACGATCACCC GCGCCAACGT TTCGGTAAAC GCGTTAAGTC gacaatcaac ctctggatta
1801caaaatttgt gaaagatt

Download FASTA (ORF) (Full)