Construct: ORF TRCN0000472901
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013003.1_s317c1
- Derived from:
- ccsbBroadEn_01266
- DNA Barcode:
- TCACCCGCGCCAACGTTTCGGTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPP3CA (5530)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472901
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5530 | PPP3CA | protein phosphatase 3 catal... | NM_001130691.2 | 100% | 100% | |
| 2 | human | 5530 | PPP3CA | protein phosphatase 3 catal... | NM_000944.5 | 98% | 98% | 1339_1368del |
| 3 | human | 5530 | PPP3CA | protein phosphatase 3 catal... | XM_017008365.1 | 95.3% | 94.4% | (many diffs) |
| 4 | human | 5530 | PPP3CA | protein phosphatase 3 catal... | NM_001130692.2 | 91.7% | 91.7% | 951_952ins126 |
| 5 | human | 5530 | PPP3CA | protein phosphatase 3 catal... | XM_024454127.1 | 88.4% | 88.4% | 0_1ins150;1189_1218del |
| 6 | mouse | 19055 | Ppp3ca | protein phosphatase 3, cata... | NM_001293622.1 | 92.5% | 99.6% | (many diffs) |
| 7 | mouse | 19055 | Ppp3ca | protein phosphatase 3, cata... | NM_008913.5 | 90.8% | 97.6% | (many diffs) |
| 8 | mouse | 19055 | Ppp3ca | protein phosphatase 3, cata... | XM_017319493.2 | 83.3% | 89.8% | (many diffs) |
| 9 | mouse | 19055 | Ppp3ca | protein phosphatase 3, cata... | XM_030252464.1 | 83.3% | 89.8% | (many diffs) |
| 10 | mouse | 19055 | Ppp3ca | protein phosphatase 3, cata... | XM_017319492.2 | 81.8% | 88% | (many diffs) |
| 11 | mouse | 19055 | Ppp3ca | protein phosphatase 3, cata... | XM_030252462.1 | 81.8% | 88% | (many diffs) |
| 12 | mouse | 19055 | Ppp3ca | protein phosphatase 3, cata... | XM_030252463.1 | 81.8% | 88% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1599
- ORF length:
- 1533
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaagaag 61 ttggcatgtc cgagcccaag gcaattgatc ccaagttgtc gacgaccgac agggtggtga 121 aagctgttcc atttcctcca agtcaccggc ttacagcaaa agaagtgttt gataatgatg 181 gaaaacctcg tgtggatatc ttaaaggcgc atcttatgaa ggagggaagg ctggaagaga 241 gtgttgcatt gagaataata acagagggtg catcaattct tcgacaggaa aaaaatttgc 301 tggatattga tgcgccagtc actgtttgtg gggacattca tggacaattc tttgatttga 361 tgaagctctt tgaagtcggg ggatctcctg ccaacactcg ctacctcttc ttaggggact 421 atgttgacag agggtacttc agtattgaat gtgtgctgta tttgtgggcc ttgaaaattc 481 tctaccccaa aacactgttt ttacttcgtg gaaatcatga atgtagacat ctaacagagt 541 atttcacatt taaacaagaa tgtaaaataa agtattcaga acgcgtatat gatgcctgta 601 tggatgcctt tgactgcctt cccctggctg ccctgatgaa ccaacagttc ctgtgtgtgc 661 atggtggttt gtctccagag attaacactt tagatgatat cagaaaatta gaccgattca 721 aagaaccacc tgcatatgga cctatgtgtg atatcctgtg gtcagacccc ctggaagatt 781 ttggaaatga gaagactcag gaacatttca ctcacaacac agtcaggggg tgttcatact 841 tctacagtta cccggctgta tgtgaattct tacagcacaa taacttgtta tctatactcc 901 gagcccacga agcccaagat gcagggtacc gcatgtacag gaaaagccaa acaacaggct 961 tcccttctct aattacaatt ttttcagcac caaattactt agatgtatac aataacaaag 1021 ctgcagtatt gaagtatgag aacaatgtta tgaatatcag gcaattcaac tgttctcctc 1081 atccatactg gcttccaaat ttcatggatg tttttacttg gTCCCTTCCA TTTGTTGGGG 1141 AAAAAGTGAC TGAGATGCTG GTAAATGTCC TCAACATCTG CTCAGATGAT GAACTAGGGT 1201 CAGAAGAAGA TGGATTTGAT GGTGCAACAG CTGCAGCCCG GAAAGAGGTG ATAAGGAACA 1261 AGATCCGAGC AATAGGCAAA ATGGCCAGAG TGTTCTCAGT GCTCAGAGAA GAGAGTGAGA 1321 GTGTGCTGAC GCTGAAAGGC TTGACCCCAA CTGGCATGCT CCCCAGCGGA GTACTTTCTG 1381 GAGGGAAGCA AACCCTGCAA AGCGCTATCA AAGGATTTTC ACCACAACAT AAGATCACTA 1441 GCTTCGAGGA AGCCAAGGGC TTAGACCGAA TTAATGAGAG GATGCCGCCT CGCAGAGATG 1501 CCATGCCCTC TGACGCCAAC CTTAACTCCA TCAACAAGGC TCTCACCTCA GAGACTAACG 1561 GCACGGACAG CAATGGCAGT AATAGCAGCA ATATTCAGTG CCCAACTTTC TTGTACAAAG 1621 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1681 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1741 ACGATCACCC GCGCCAACGT TTCGGTAAAC GCGTTAAGTC gacaatcaac ctctggatta 1801 caaaatttgt gaaagatt