Construct: ORF TRCN0000472904
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014126.1_s317c1
- Derived from:
- ccsbBroadEn_07056
- DNA Barcode:
- TTTACCGTTTCTGAGATTACTGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TGFB1 (7040)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472904
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7040 | TGFB1 | transforming growth factor ... | NM_000660.7 | 99.9% | 99.7% | 29C>T |
| 2 | human | 7040 | TGFB1 | transforming growth factor ... | XM_011527242.2 | 99.6% | 99.4% | 29C>T;712_714delGCA |
| 3 | mouse | 21803 | Tgfb1 | transforming growth factor,... | NM_011577.2 | 85.1% | 89.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1236
- ORF length:
- 1170
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc gccctccggg ctgcggctgc tgctgctgct gctaccgctg ctgtggctac 121 tggtgctgac gcctggccgg ccggccgcgg gactatccac ctgcaagact atcgacatgg 181 agctggtgaa gcggaagcgc atcgaggcca tccgcggcca gatcctgtcc aagctgcggc 241 tcgccagccc cccgagccag ggggaggtgc cgcccggccc gctgcccgag gccgtgctcg 301 ccctgtacaa cagcacccgc gaccgggtgg ccggggagag tgcagaaccg gagcccgagc 361 ctgaggccga ctactacgcc aaggaggtca cccgcgtgct aatggtggaa acccacaacg 421 aaatctatga caagttcaag cagagtacac acagcatata tatgttcttc aacacatcag 481 agctccgaga agcggtacct gaacccgtgt tgctctcccg ggcagagctg cgtctgctga 541 ggctcaagtt aaaagtggag cagcacgtgg agctgtacca gaaatacagc aacaattcct 601 ggcgatacct cagcaaccgg ctgctggcac ccagcgactc gccagagtgg ttatcttttg 661 atgtcaccgg agttgtgcgg cagtggttga gccgtggagg ggaaattgag ggctttcgcc 721 ttagcgccca ctgctcctgt gacagcaggg ataacacact gcaagtggac atcaacgggt 781 tcactaccgg ccgccgaggt gacctggcca ccattcatgg catgaaccgg cctttccTGC 841 TTCTCATGGC CACCCCGCTG GAGAGGGCCC AGCATCTGCA AAGCTCCCGG CACCGCCGAG 901 CCCTGGACAC CAACTATTGC TTCAGCTCCA CGGAGAAGAA CTGCTGCGTG CGGCAGCTGT 961 ACATTGACTT CCGCAAGGAC CTCGGCTGGA AGTGGATCCA CGAGCCCAAG GGCTACCATG 1021 CCAACTTCTG CCTCGGGCCC TGCCCCTACA TTTGGAGCCT GGACACGCAG TACAGCAAGG 1081 TCCTGGCCCT GTACAACCAG CATAACCCGG GCGCCTCGGC GGCGCCGTGC TGCGTGCCGC 1141 AGGCGCTGGA GCCGCTGCCC ATCGTGTACT ACGTGGGCCG CAAGCCCAAG GTGGAGCAGC 1201 TGTCCAACAT GATCGTGCGC TCCTGCAAGT GCAGCTGCCC AACTTTCTTG TACAAAGTGG 1261 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1321 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1381 ATTTACCGTT TCTGAGATTA CTGCCACGCG TTAAGTCgac aatcaacctc tggattacaa 1441 aatttgtgaa agatt