Construct: ORF TRCN0000472908

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017821.1_s317c1
Derived from:
ccsbBroadEn_07104
DNA Barcode:
CCGATTATGCCCAACTGTCGGTTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TSHR (7253)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000472908
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7253 TSHR thyroid stimulating hormone... NM_001018036.3 99.7% 99.6% 696A>G;742C>A
2 human 7253 TSHR thyroid stimulating hormone... XM_005268039.1 99.7% 99.6% 696A>G;742C>A
3 human 7253 TSHR thyroid stimulating hormone... NM_001142626.3 92% 91.9% 693_755del;759A>G;805C>A
4 human 7253 TSHR thyroid stimulating hormone... XM_006720245.1 92% 91.9% 693_755del;759A>G;805C>A
5 human 7253 TSHR thyroid stimulating hormone... NM_000369.3 32.4% 30.1% (many diffs)
6 human 7253 TSHR thyroid stimulating hormone... XM_005268037.4 32.4% 30.1% (many diffs)
7 human 7253 TSHR thyroid stimulating hormone... XM_011537119.2 19.9% 16.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
825
ORF length:
759
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgag gccggcggac ttgctgcagc tggtgctgct gctcgacctg cccagggacc
121tgggcggaat ggggtgttcg tctccaccct gcgagtgcca tcaggaggag gacttcagag
181tcacctgcaa ggatattcaa cgcatcccca gcttaccgcc cagtacgcag actctgaagc
241ttattgagac tcacctgaga actattccaa gtcatgcatt ttctaatctg cccaatattt
301ccagaatcta cgtatctata gatgtgactc tgcagcagct ggaatcacac tccTTCTACA
361ATTTGAGTAA AGTGACTCAC ATAGAAATTC GGAATACCAG GAACTTAACT TACATAGACC
421CTGATGCCCT CAAAGAGCTC CCCCTCCTAA AGTTCCTTGG CATTTTCAAC ACTGGACTTA
481AAATGTTCCC TGACCTGACC AAAGTTTATT CCACTGATAT ATTCTTTATA CTTGAAATTA
541CAGACAACCC TTACATGACG TCAATCCCTG TGAATGCTTT TCAGGGACTA TGCAATGAAA
601CCTTGACACT GAAGCTGTAC AACAATGGCT TTACTTCAGT CCAAGGATAT GCTTTCAATG
661GGACAAAGCT GGATGCTGTT TACCTAAACA AGAATAAATA CCTGACAGTT ATTGACAAAG
721ATGCATTTGG AGGAGTATAC AGTGGACCAA GCTTGCTGCT GCCTCTTGGA AGAAAGTCCT
781TGTCCTTTGA GACTCAGAAG GCCCCAAGCT CCAGTATGCC ATCATGCCCA ACTTTCTTGT
841ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
901AGTAATGAAC TAGTCCGTAA CTTGAAGGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
961GAAAGGACGA CCGATTATGC CCAACTGTCG GTTTACGCGT TAAGTCgaca atcaacctct
1021ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)