Construct: ORF TRCN0000472926

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019026.3_s317c1
Derived from:
ccsbBroadEn_14643
DNA Barcode:
TTACTCCGCGAACAAACAATAACA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
FLT1 (2321)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000472926
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2321 FLT1 fms related tyrosine kinase 1 NM_002019.4 100% 100%
2 human 2321 FLT1 fms related tyrosine kinase 1 XM_017020485.1 97.1% 97.1% 2591_2592ins114
3 human 2321 FLT1 fms related tyrosine kinase 1 NM_001160030.2 54.2% 53.2% (many diffs)
4 human 2321 FLT1 fms related tyrosine kinase 1 XM_011535014.1 53.2% 52.7% (many diffs)
5 human 2321 FLT1 fms related tyrosine kinase 1 NM_001159920.2 50.7% 49% (many diffs)
6 human 2321 FLT1 fms related tyrosine kinase 1 NM_001160031.1 40% 38.7% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
4083
ORF length:
4014
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggtcagctac tgggacaccg gggtcctgct gtgcgcgctg ctcagctgtc
121tgcttctcac aggatctagt tcaggttcaa aattaaaaga tcctgaactg agtttaaaag
181gcacccagca catcatgcaa gcaggccaga cactgcatct ccaatgcagg ggggaagcag
241cccataaatg gtctttgcct gaaatggtga gtaaggaaag cgaaaggctg agcataacta
301aatctgcctg tggaagaaat ggcaaacaat tctgcagtac tttaaccttg aacacagctc
361aagcaaacca cactggcttc tacagctgca aatatctagc tgtacctact tcaaagaaga
421aggaaacaga atctgcaatc tatatattta ttagtgatac aggtagacct ttcgtagaga
481tgtacagtga aatccccgaa attatacaca tgactgaagg aagggagctc gtcattccct
541gccgggttac gtcacctaac atcactgtta ctttaaaaaa gtttccactt gacactttga
601tccctgatgg aaaacgcata atctgggaca gtagaaaggg cttcatcata tcaaatgcaa
661cgtacaaaga aatagggctt ctgacctgtg aagcaacagt caatgggcat ttgtataaga
721caaactatct cacacatcga caaaccaata caatcataga tgtccaaata agcacaccac
781gcccagtcaa attacttaga ggccatactc ttgtcctcaa ttgtactgct accactccct
841tgaacacgag agttcaaatg acctggagtt accctgatga aaaaaataag agagcttccg
901taaggcgacg aattgaccaa agcaattccc atgccaacat attctacagt gttcttacta
961ttgacaaaat gcagaacaaa gacaaaggac tttatacttg tcgtgtaagg agtggaccat
1021cattcaaatc tgttaacacc tcagtgcata tatatgataa agcattcatc actgtgaaac
1081atcgaaaaca gcaggtgctt gaaaccgtag ctggcaagcg gtcttaccgg ctctctatga
1141aagtgaaggc atttccctcg ccggaagttg tatggttaaa agatgggtta cctgcgactg
1201agaaatctgc tcgctatttg actcgtggct actcgttaat tatcaaggac gtaactgaag
1261aggatgcagg gaattataca atcttgctga gcataaaaca gtcaaatgtg tttaaaaacc
1321tcactgccac tctaattgtc aatgtgaaac cccagattta cgaaaaggcc gtgtcatcgt
1381ttccagaccc ggctctctac ccactgggca gcagacaaat cctgacttgt accgcatatg
1441gtatccctca acctacaatc aagtggttct ggcacccctg taaccataat cattccgaag
1501caaggtgtga cttttgttcc aataatgaag agtcctttat cctggatgct gacagcaaca
1561tgggaaacag aattgagagc atcactcagc gcatggcaat aatagaagga aagaataaga
1621tggctagcac cttggttgtg gctgactcta gaatttctgg aatctacatt tgcatagctt
1681ccaataaagt tgggactgtg ggaagaaaca taagctttta tatcacagat gtgccaaatg
1741ggtttcatgt taacttggaa aaaatgccga cggaaggaga ggacctgaaa ctgtcttgca
1801cagttaacaa gttcttatac agagacgtta cttggatttt actgcggaca gttaataaca
1861gaacaatgca ctacagtatt agcaagcaaa aaatggccat cactaaggag cactccatca
1921ctcttaatct taccatcatg aatgtttccc tgcaagattc aggcacctat gcctgcagag
1981ccaggaatgt atacacaggg gaagaaatcc tccagaagaa agaaattaca atcagagatc
2041aggaagcacc atacctcctg cgaaacctca gtgatcacac agtggccatc agcagttcca
2101ccactttaga ctgtcatgct aatggtgtcc ccgagcctca gatcacttgg tttaaaaaca
2161accacaaaat acaacaagag cctggaatta ttttaggacc aggaagcagc acgctgttta
2221ttgaaagagt cacagaagag gatgaaggtg tctatcactg caaagccacc aaccagaagg
2281gctctgtgga aagttcagca tacctcactg ttcaaggaac ctcggacaag tctaatctgg
2341agctgatcac tctaacatgc acctgtgtgg ctgcgactct cttctggctc ctattaaccc
2401tctttatccg aaaaatgaaa aggtcttctt ctgaaataaa gactgactac ctatcaatta
2461taatggaccc agatgaagtt cctttggatg agcagtgtga gcggctccct tatgatgcca
2521gcaagtggga gtttgcccgg gagagactta aactgggcaa atcacttgga agaggggctt
2581ttggaaaagt ggttcaagca tcagcatttg gcattaagaa atcacctacg tgccggactg
2641tggctgtgaa aatgctgaaa gagggggcca cggccagcga gtacaaagct ctgatgactg
2701agctaaaaat cttgacccac attggccacc atctgaacgt ggttaacctg ctgggagcct
2761gcaccaagca aggagggcct ctgatggtga ttgttgaata ctgcaaatat ggaaatctct
2821ccaactacct caagagcaaa cgtgacttat tttttctcaa caaggatgca gcactacaca
2881tggagcctaa gaaagaaaaa atggagccag gcctggaaca aggcaagaaa ccaagactag
2941atagcgtcac cagcagcgaa agctttgcga gctccggctt tcaggaagat aaaagtctga
3001gtgatgttga ggaagaggag gattctgacg gtttctacaa ggagcccatc actatggaag
3061atctgatttc ttacagtttt caagtggcca gaggcatgga gttcctgtct tccagaaagt
3121gcattcatcg ggacctggca gcgagaaaca ttcttttatc tgagaacaac gtggtgaaga
3181tttgtgattt tggccttgcc cgggatattt ataagaaccc cgattatgtg agaaaaggag
3241atactcgact tcctctgaaa tggatggctc ctgaatctat ctttgacaaa atctacagca
3301ccaagagcga cgtgtggtct tacggagtat tgctgtggga aatcttctcc ttaggtgggt
3361ctccataccc aggagtacaa atggatgagg acttttgcag tcgcctgagg gaaggcatga
3421ggatgagagc tcctgagtac tctactcctg aaatctatca gatcatgctg gactgctggc
3481acagagaccc aaaagaaagg ccaagatttg cagaacttgt ggaaaaacta ggtgatttgc
3541ttcaagcaaa tgtacaacag gatggtaaag actacatccc aatcaatgcc atactgacag
3601gaaatagtgg gtttacatac tcaactcctg ccttctctga ggacttcttc aaggaaagta
3661tttcagctcc gaagtttaat tcaggaagct ctgatgatgT CAGATACGTA AATGCTTTCA
3721AGTTCATGAG CCTGGAAAGA ATCAAAACCT TTGAAGAACT TTTACCGAAT GCCACCTCCA
3781TGTTTGATGA CTACCAGGGC GACAGCAGCA CTCTGTTGGC CTCTCCCATG CTGAAGCGCT
3841TCACCTGGAC TGACAGCAAA CCCAAGGCCT CGCTCAAGAT TGACTTGAGA GTAACCAGTA
3901AAAGTAAGGA GTCGGGGCTG TCTGATGTCA GCAGGCCCAG TTTCTGCCAT TCCAGCTGTG
3961GGCACGTCAG CGAAGGCAAG CGCAGGTTCA CCTACGACCA CGCTGAGCTG GAAAGGAAAA
4021TCGCGTGCTG CTCCCCGCCC CCAGACTACA ACTCGGTGGT CCTGTACTCC ACCCCACCCA
4081TCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
4141GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
4201TTTATATATC TTGTGGAAAG GACGATTACT CCGCGAACAA ACAATAACAA CGCGTTAAGT
4261Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)