Construct: ORF TRCN0000472930
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003290.3_s317c1
- Derived from:
- ccsbBroadEn_14897
- DNA Barcode:
- GTTACTAACTGCTTGTCGTGATCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAP4K3 (8491)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472930
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8491 | MAP4K3 | mitogen-activated protein k... | NM_001270425.2 | 99.9% | 100% | 189T>C;1647A>T |
| 2 | human | 8491 | MAP4K3 | mitogen-activated protein k... | NM_003618.4 | 97.5% | 97.6% | 189T>C;1057_1119del;1710A>T |
| 3 | human | 8491 | MAP4K3 | mitogen-activated protein k... | XR_939730.2 | 58.8% | (many diffs) | |
| 4 | human | 8491 | MAP4K3 | mitogen-activated protein k... | XM_024453183.1 | 51.1% | 51.2% | 0_1ins1278;369A>T |
| 5 | mouse | 225028 | Map4k3 | mitogen-activated protein k... | XM_006524197.2 | 88.7% | 96.1% | (many diffs) |
| 6 | mouse | 225028 | Map4k3 | mitogen-activated protein k... | XM_006524195.2 | 87.6% | 94.9% | (many diffs) |
| 7 | mouse | 225028 | Map4k3 | mitogen-activated protein k... | XM_006524198.2 | 87% | 94.1% | (many diffs) |
| 8 | mouse | 225028 | Map4k3 | mitogen-activated protein k... | NM_001290345.1 | 86.6% | 93.8% | (many diffs) |
| 9 | mouse | 225028 | Map4k3 | mitogen-activated protein k... | XM_006524192.2 | 85.5% | 92.7% | (many diffs) |
| 10 | mouse | 225028 | Map4k3 | mitogen-activated protein k... | XM_006524196.2 | 84.9% | 91.9% | (many diffs) |
| 11 | mouse | 225028 | Map4k3 | mitogen-activated protein k... | XM_006524194.2 | 83.9% | 90.8% | (many diffs) |
| 12 | mouse | 225028 | Map4k3 | mitogen-activated protein k... | XM_006524199.3 | 76.2% | 82.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2685
- ORF length:
- 2619
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa ccccggcttc gatttgtccc gccggaaccc gcaggaggac ttcgagctga 121 ttcagcgcat cggcagcggc acctacggcg acgtctacaa ggcacggaat gttaacactg 181 gtgaattagc agcaattaaa gtaataaaat tggaaccagg agaagacttt gcagttgtgc 241 agcaagaaat tatcatgatg aaagactgta aacacccaaa tattgttgct tattttggaa 301 gctatctcag gcgagataag ctttggattt gcatggagtt ttgtggaggt ggttctttac 361 aggatattta tcacgtaact ggacctctgt cagaactgca aattgcatat gttagcagag 421 aaacactgca gggattatat tatcttcaca gtaaaggaaa aatgcacaga gatataaagg 481 gagctaacat tctattaacg gataatggtc atgtgaaatt ggctgatttt ggagtatctg 541 cacagataac agctacaatt gccaaacgga agtctttcat tggcacacca tattggatgg 601 ctccagaagt tgcagctgtt gagaggaagg ggggttacaa tcaactctgt gatctctggg 661 cagtgggaat cactgccata gaacttgcag agcttcagcc tcctatgttt gacttacacc 721 caatgagagc attatttcta atgacaaaaa gcaattttca gcctcctaaa ctaaaggata 781 aaatgaaatg gtcaaatagt tttcatcact ttgtgaaaat ggcacttacc aaaaatccga 841 aaaaaagacc tactgctgaa aaattattac agcatccttt tgtaacacaa catttgacac 901 ggtctttggc aatcgagctg ttggataaag taaataatcc agatcattcc acttaccatg 961 atttcgatga tgatgatcct gagcctcttg ttgctgtacc acatagaatt cactcaacaa 1021 gtagaaacgt gagagaagaa aaaacacgct cagagataac ctttggccaa gtgaaatttg 1081 atccaccctt aagaaaggag acagaaccac atcatgaact tgatctgcaa ctggaatatg 1141 gacaaggaca ccaaggtggt tactttttag gtgcaaacaa gagtcttctc aagtctgttg 1201 aagaagaatt gcatcagcga ggacacgtcg cacatttaga agatgatgaa ggagatgatg 1261 atgaatctaa acactcaact ctgaaagcaa aaattccacc tcctttgcca ccaaagccta 1321 agtctatctt cataccacag gaaatgcatt ctactgagga tgaaaatcaa ggaacaatca 1381 agagatgtcc catgtcaggg agcccagcaa agccatccca agttccacct agaccaccac 1441 ctcccagatt acccccacac aaacctgttg ccttaggaaa tggaatgagc tccttccagt 1501 taaatggtga acgagatggc tcattatgtc aacaacagaa tgaacataga ggcacaaacc 1561 tttcaagaaa agaaaagaaa gatgtaccaa agcctattag taatggtctt cctccaacac 1621 ctaaagtgca tatgggtgca tgtttttcaa aagtttttaa tgggtgtccc ttgaaaattc 1681 actgtgcatc atcatggata aacccagata ctagagatca gtacttgata tttggtgccg 1741 aagaagggat ttataccctc aatcttaatg aacttcatga aacatcaatg gaacagctat 1801 tccctcgaag gtgtacatgg ttgtatgtaa tgaacaattg cttgctatca atatctggta 1861 aagcttctca gctttattcc cataatttac cagggctttt tgattatgca agacaaatgc 1921 aaaagttacc tgttgctatt ccagcacaca aactccctga cagaatactg ccaaggaaat 1981 tttctgtatc agcaaaaatc cctgaaacca aatggtgcca gaagtgttgt gttgtaagaa 2041 atccttacac gggccataaa tacctatgtg gagcacttca gactagcatt gttctattag 2101 aatgggttga accaatgcag aaatttatgt taattaagca catagatttt cctataccat 2161 gtccacttag aatgtttgaa atgctggtag ttcctgaaca ggagtaccct ttagtttgtg 2221 ttggtgtcag tagaggtaga gacttcaacc aagtggttcg atttgagacg gtcaatccaa 2281 attctacctc ttcatggttt acagaaTCAG ATACCCCACA GACAAATGTT ACTCATGTAA 2341 CCCAACTGGA GAGAGATACC ATCCTTGTAT GCTTGGACTG TTGTATAAAA ATAGTAAATC 2401 TCCAAGGAAG ATTAAAATCT AGCAGGAAAT TGTCATCAGA ACTCACCTTT GATTTCCAGA 2461 TTGAATCAAT AGTGTGCCTA CAAGACAGTG TGCTAGCTTT CTGGAAACAT GGAATGCAAG 2521 GTAGAAGTTT TAGATCTAAT GAGGTAACAC AAGAAATTTC AGATAGCACA AGAATTTTCA 2581 GGCTGCTTGG ATCTGACAGG GTCGTGGTTT TGGAAAGTAG GCCAACTGAT AACCCCACAG 2641 CAAATAGCAA TTTGTACATC CTGGCGGGTC ATGAAAACAG TTACTGCCCA ACTTTCTTGT 2701 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 2761 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 2821 GAAAGGACGA GTTACTAACT GCTTGTCGTG ATCAACGCGT TAAGTCgaca atcaacctct 2881 ggattacaaa atttgtgaaa gatt