Construct: ORF TRCN0000472940
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018344.1_s317c1
- Derived from:
- ccsbBroadEn_05547
- DNA Barcode:
- TCTGCCAGAAGTTCTTTTGAGCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STIMATE (375346)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472940
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 375346 | STIMATE | STIM activating enhancer | NM_198563.3 | 100% | 100% | |
| 2 | human | 100526772 | STIMATE-MUSTN1 | STIMATE-MUSTN1 readthrough | NM_001198974.3 | 78.8% | 78.7% | 881_1116delinsTA |
| 3 | mouse | 69179 | Tmem110 | transmembrane protein 110 | NM_028839.4 | 89.9% | 95.5% | (many diffs) |
| 4 | mouse | 69179 | Tmem110 | transmembrane protein 110 | XM_011245171.1 | 72.6% | 75.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 948
- ORF length:
- 882
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca gggccccgcc gggaacgcga gccggggact gccaggcggg ccgccctcca 121 cagtcgcgtc cggggcgggc cgctgcgaga gcggcgcgct catgcacagc ttcggcatct 181 tcctgcaggg gctgctcggc gtcgtggcct tcagcacgtt aatgctcaaa cgcttcagag 241 aaccaaagca tgaaagacgt ccgtggagga tatggttttt agacacttcc aaacaagcca 301 taggaatgct gttcatccac tttgcaaatg tatacctagc agatctcact gaagaggacc 361 cttgttcact gtacctcatc aacttcctcc tggacgccac tgtgggcatg ctgctcatct 421 acgtgggggt gcgcgccgtc agcgtcctgg tagagtggca gcagtgggag tccctgcgct 481 tcggcgaata tggagaccct ctgcagtgtg gagccTGGGT CGGGCAGTGC GCTCTTTACA 541 TCGTGATCAT GATTTTTGAA AAGTCTGTCG TCTTCATCGT CCTCCTAATA CTTCAGTGGA 601 AAAAGGTGGC CCTGTTGAAT CCCATTGAAA ACCCAGACTT GAAGCTGGCC ATCGTCATGC 661 TGATCGTCCC CTTCTTTGTC AACGCTTTGA TGTTTTGGGT AGTGGACAAT TTCCTCATGA 721 GAAAGGGGAA GACGAAAGCT AAGCTAGAAG AAAGGGGAGC CAACCAGGAC TCGAGGAATG 781 GGAGCAAGGT CCGCTACCGG AGGGCCGCAT CCCACGAGGA GTCTGAGTCT GAGATCCTGA 841 TCTCAGCGGA TGATGAGATG GAGGAGTCCG ACGTGGAGGA GGACCTCCGC AGACTGACCC 901 CCCTCAAGCC TGTGAAGAAA AAGAAGCACC GCTTTGGGCT ACCCGTATGC CCAACTTTCT 961 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1021 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1081 GTGGAAAGGA CGATCTGCCA GAAGTTCTTT TGAGCCAACG CGTTAAGTCg acaatcaacc 1141 tctggattac aaaatttgtg aaagatt