Construct: ORF TRCN0000472945
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008134.1_s317c1
- Derived from:
- ccsbBroadEn_09156
- DNA Barcode:
- CCCTTGTTACGGGATAATCAATTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- METTL25 (84190)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472945
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84190 | METTL25 | methyltransferase like 25 | NM_032230.3 | 99.8% | 99.8% | 498C>T;745C>A |
| 2 | human | 84190 | METTL25 | methyltransferase like 25 | XM_011538827.3 | 90.7% | 90.7% | 258_259ins165;333C>T;580C>A |
| 3 | human | 84190 | METTL25 | methyltransferase like 25 | NM_001347934.2 | 89.6% | 84.9% | (many diffs) |
| 4 | human | 84190 | METTL25 | methyltransferase like 25 | XM_011538829.3 | 87.9% | 86.8% | (many diffs) |
| 5 | human | 84190 | METTL25 | methyltransferase like 25 | XM_011538828.3 | 87.7% | 87.3% | (many diffs) |
| 6 | human | 84190 | METTL25 | methyltransferase like 25 | NM_001319675.2 | 85.4% | 85.4% | 0_1ins261;237C>T;484C>A |
| 7 | human | 84190 | METTL25 | methyltransferase like 25 | XR_001748891.2 | 85.3% | (many diffs) | |
| 8 | human | 84190 | METTL25 | methyltransferase like 25 | NR_144940.2 | 83.9% | (many diffs) | |
| 9 | human | 84190 | METTL25 | methyltransferase like 25 | XR_001748893.2 | 82.9% | (many diffs) | |
| 10 | human | 84190 | METTL25 | methyltransferase like 25 | XR_001748895.2 | 80.8% | (many diffs) | |
| 11 | human | 84190 | METTL25 | methyltransferase like 25 | NR_144942.2 | 80.3% | (many diffs) | |
| 12 | human | 84190 | METTL25 | methyltransferase like 25 | XR_944768.3 | 76.4% | (many diffs) | |
| 13 | human | 84190 | METTL25 | methyltransferase like 25 | XR_001748898.2 | 76% | (many diffs) | |
| 14 | human | 84190 | METTL25 | methyltransferase like 25 | XM_017020021.1 | 75% | 74.9% | 0_1ins450;48C>T;295C>A |
| 15 | human | 84190 | METTL25 | methyltransferase like 25 | NR_144941.2 | 73.3% | (many diffs) | |
| 16 | human | 84190 | METTL25 | methyltransferase like 25 | XM_005269186.5 | 72% | 72% | (many diffs) |
| 17 | human | 84190 | METTL25 | methyltransferase like 25 | NR_144943.2 | 71.8% | (many diffs) | |
| 18 | human | 84190 | METTL25 | methyltransferase like 25 | XR_001748897.2 | 70.3% | (many diffs) | |
| 19 | human | 84190 | METTL25 | methyltransferase like 25 | XR_001748894.2 | 50.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1875
- ORF length:
- 1809
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcttcttgc cctctcccgg tgaccccgga cctgcccacg ctgcgtgcca 121 agttgcaggg actgctgcag ttcctgaggg atgccctgtc catttccaat gcacataccg 181 tggatttcta cacagaatcc gtgtgggagg agctggtcga cttgccaccg gagacagtgc 241 tggctgcgct gaggaagtca gcgtcggaga cggaggccct gccctcagag acgcgccccc 301 tagtggaagc agagtgggaa gcaggtatga ctgattttcc caaaatattt tgtgaaactt 361 ctcagaagtt ggtgagtgtg gaagcctttg ctctggctgc gaaatactat tctgtacaaa 421 acttgggaat atgtactcct tttgaacagt tgcttgtagc ccttcgagga aatcaaaacc 481 agagaattgg tgaaaatcag aaggcagttg agtttatgaa tatgaagaaa tctcatgaag 541 ttcaggcaat gtcagagctg attagcagta ttgctgacta ctatggaata aagcaggtga 601 ttgacttggg ttccggtaaa ggctacctaa gctctttttt gtccttgaag tatggcttaa 661 aagtttatgg aattgattct tcaaatacca atactcatgg agctgaggag agaaacagaa 721 aattgaagaa acattggaaa ctctgtcatg ctcagtcaag attagatgtc aatggactag 781 cattaaaaat ggcaaaagaa aggaaagtga aaaataaagt taaaaataaa gctgatactg 841 aggaagtgtt taacaacagt cctacaaatc aagaaaagat gcctacctca gctattttgc 901 ctgatttttc tggctctgta atttctaata tcagaaacca aatggaaacc cttcattctc 961 agccacatca agaagaaaat ttgtgttttg aaaattcctt ttctcttata aaccttttgc 1021 ctattaatgc tgtagagcct acttcttcac agcaaatacc caacagagaa acatctgaag 1081 ccaataaaga gagaagaaaa atgacatcaa agtcaagtga atcaaatata tattcacctt 1141 taacctcttt tatcactgct gattcagaac tccatgacat tattaaagat ttggaggatt 1201 gtttgatggt gggtctccac acttgtggtg atctggctcc aaatactttg cgaatattta 1261 cctccaactc tgaaatcaag ggagtttgca gtgtgggttg ttgctaccac ctcttatctg 1321 aagaatttga aaaccagcat aaagaacgta ctcaggaaaa gtggggattt ccaatgtgcc 1381 actatttaaa ggaagagaga tggtgctgtg gtcgtaatgc cagaatgtca gcatgtttgg 1441 cattggagcg ggttgcagct ggccaagggc tgcctactga atcactcttc tatcgtgctg 1501 ttcttcagga tattattaaa gattgttatg gcaTCACCAA ATGTGATCGG CATGTTGGTA 1561 AAATTTATTC CAAATGTTCT TCTTTTCTGG ATTATGTCAG ACGGTCTCTA AAGAAGCTTG 1621 GATTAGATGA GTCCAAGCTG CCAGAAAAAA TTATAATGAA CTACTACGAG AAGTATAAGC 1681 CTCGAATGAA TGAGCTGGAA GCTTTTAATA TGTTGAAAGT TGTACTGGCT CCCTGTATAG 1741 AGACTTTGAT TCTTCTGGAT CGACTTTGTT ACCTGAAAGA GCAGGAAGAT ATTGCATGGT 1801 CTGCTCTTGT GAAGTTGTTT GATCCCGTGA AATCTCCCAG ATGTTATGCT GTTATTGCCC 1861 TGAAGAAGCA GCAGTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1921 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1981 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CCCTTGTTAC GGGATAATCA 2041 ATTCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt