Construct: ORF TRCN0000472945

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008134.1_s317c1
Derived from:
ccsbBroadEn_09156
DNA Barcode:
CCCTTGTTACGGGATAATCAATTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
METTL25 (84190)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000472945
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 84190 METTL25 methyltransferase like 25 NM_032230.3 99.8% 99.8% 498C>T;745C>A
2 human 84190 METTL25 methyltransferase like 25 XM_011538827.3 90.7% 90.7% 258_259ins165;333C>T;580C>A
3 human 84190 METTL25 methyltransferase like 25 NM_001347934.2 89.6% 84.9% (many diffs)
4 human 84190 METTL25 methyltransferase like 25 XM_011538829.3 87.9% 86.8% (many diffs)
5 human 84190 METTL25 methyltransferase like 25 XM_011538828.3 87.7% 87.3% (many diffs)
6 human 84190 METTL25 methyltransferase like 25 NM_001319675.2 85.4% 85.4% 0_1ins261;237C>T;484C>A
7 human 84190 METTL25 methyltransferase like 25 XR_001748891.2 85.3% (many diffs)
8 human 84190 METTL25 methyltransferase like 25 NR_144940.2 83.9% (many diffs)
9 human 84190 METTL25 methyltransferase like 25 XR_001748893.2 82.9% (many diffs)
10 human 84190 METTL25 methyltransferase like 25 XR_001748895.2 80.8% (many diffs)
11 human 84190 METTL25 methyltransferase like 25 NR_144942.2 80.3% (many diffs)
12 human 84190 METTL25 methyltransferase like 25 XR_944768.3 76.4% (many diffs)
13 human 84190 METTL25 methyltransferase like 25 XR_001748898.2 76% (many diffs)
14 human 84190 METTL25 methyltransferase like 25 XM_017020021.1 75% 74.9% 0_1ins450;48C>T;295C>A
15 human 84190 METTL25 methyltransferase like 25 NR_144941.2 73.3% (many diffs)
16 human 84190 METTL25 methyltransferase like 25 XM_005269186.5 72% 72% (many diffs)
17 human 84190 METTL25 methyltransferase like 25 NR_144943.2 71.8% (many diffs)
18 human 84190 METTL25 methyltransferase like 25 XR_001748897.2 70.3% (many diffs)
19 human 84190 METTL25 methyltransferase like 25 XR_001748894.2 50.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1875
ORF length:
1809
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc ggcttcttgc cctctcccgg tgaccccgga cctgcccacg ctgcgtgcca
121agttgcaggg actgctgcag ttcctgaggg atgccctgtc catttccaat gcacataccg
181tggatttcta cacagaatcc gtgtgggagg agctggtcga cttgccaccg gagacagtgc
241tggctgcgct gaggaagtca gcgtcggaga cggaggccct gccctcagag acgcgccccc
301tagtggaagc agagtgggaa gcaggtatga ctgattttcc caaaatattt tgtgaaactt
361ctcagaagtt ggtgagtgtg gaagcctttg ctctggctgc gaaatactat tctgtacaaa
421acttgggaat atgtactcct tttgaacagt tgcttgtagc ccttcgagga aatcaaaacc
481agagaattgg tgaaaatcag aaggcagttg agtttatgaa tatgaagaaa tctcatgaag
541ttcaggcaat gtcagagctg attagcagta ttgctgacta ctatggaata aagcaggtga
601ttgacttggg ttccggtaaa ggctacctaa gctctttttt gtccttgaag tatggcttaa
661aagtttatgg aattgattct tcaaatacca atactcatgg agctgaggag agaaacagaa
721aattgaagaa acattggaaa ctctgtcatg ctcagtcaag attagatgtc aatggactag
781cattaaaaat ggcaaaagaa aggaaagtga aaaataaagt taaaaataaa gctgatactg
841aggaagtgtt taacaacagt cctacaaatc aagaaaagat gcctacctca gctattttgc
901ctgatttttc tggctctgta atttctaata tcagaaacca aatggaaacc cttcattctc
961agccacatca agaagaaaat ttgtgttttg aaaattcctt ttctcttata aaccttttgc
1021ctattaatgc tgtagagcct acttcttcac agcaaatacc caacagagaa acatctgaag
1081ccaataaaga gagaagaaaa atgacatcaa agtcaagtga atcaaatata tattcacctt
1141taacctcttt tatcactgct gattcagaac tccatgacat tattaaagat ttggaggatt
1201gtttgatggt gggtctccac acttgtggtg atctggctcc aaatactttg cgaatattta
1261cctccaactc tgaaatcaag ggagtttgca gtgtgggttg ttgctaccac ctcttatctg
1321aagaatttga aaaccagcat aaagaacgta ctcaggaaaa gtggggattt ccaatgtgcc
1381actatttaaa ggaagagaga tggtgctgtg gtcgtaatgc cagaatgtca gcatgtttgg
1441cattggagcg ggttgcagct ggccaagggc tgcctactga atcactcttc tatcgtgctg
1501ttcttcagga tattattaaa gattgttatg gcaTCACCAA ATGTGATCGG CATGTTGGTA
1561AAATTTATTC CAAATGTTCT TCTTTTCTGG ATTATGTCAG ACGGTCTCTA AAGAAGCTTG
1621GATTAGATGA GTCCAAGCTG CCAGAAAAAA TTATAATGAA CTACTACGAG AAGTATAAGC
1681CTCGAATGAA TGAGCTGGAA GCTTTTAATA TGTTGAAAGT TGTACTGGCT CCCTGTATAG
1741AGACTTTGAT TCTTCTGGAT CGACTTTGTT ACCTGAAAGA GCAGGAAGAT ATTGCATGGT
1801CTGCTCTTGT GAAGTTGTTT GATCCCGTGA AATCTCCCAG ATGTTATGCT GTTATTGCCC
1861TGAAGAAGCA GCAGTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC
1921CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA
1981TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CCCTTGTTAC GGGATAATCA
2041ATTCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)