Construct: ORF TRCN0000472955
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001894.1_s317c1
- Derived from:
- ccsbBroadEn_12198
- DNA Barcode:
- AACAAGGGTAGCCGCACGAGGCGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UBE2W (55284)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472955
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55284 | UBE2W | ubiquitin conjugating enzym... | NM_001271015.3 | 99.3% | 98.1% | 58C>T;89T>C;127G>A |
| 2 | human | 55284 | UBE2W | ubiquitin conjugating enzym... | NM_018299.6 | 93.9% | 90.5% | (many diffs) |
| 3 | human | 55284 | UBE2W | ubiquitin conjugating enzym... | NM_001001481.4 | 87.8% | 84.7% | (many diffs) |
| 4 | human | 55284 | UBE2W | ubiquitin conjugating enzym... | NR_073121.3 | 4.3% | (many diffs) | |
| 5 | human | 55284 | UBE2W | ubiquitin conjugating enzym... | NR_073120.3 | 4.2% | (many diffs) | |
| 6 | human | 55284 | UBE2W | ubiquitin conjugating enzym... | NR_073119.3 | 4.1% | (many diffs) | |
| 7 | mouse | 66799 | Ube2w | ubiquitin-conjugating enzym... | NM_025773.3 | 74.8% | 76% | (many diffs) |
| 8 | mouse | 66799 | Ube2w | ubiquitin-conjugating enzym... | XM_006495557.3 | 68.8% | 63.2% | (many diffs) |
| 9 | mouse | 66799 | Ube2w | ubiquitin-conjugating enzym... | XM_017322047.1 | 64.7% | 63.2% | (many diffs) |
| 10 | mouse | 66799 | Ube2w | ubiquitin-conjugating enzym... | NM_001271017.1 | 61.4% | 40.8% | (many diffs) |
| 11 | mouse | 66799 | Ube2w | ubiquitin-conjugating enzym... | NM_001271016.1 | 57.5% | 35.9% | (many diffs) |
| 12 | mouse | 66799 | Ube2w | ubiquitin-conjugating enzym... | XM_011238402.2 | 48.2% | 41% | (many diffs) |
| 13 | mouse | 66799 | Ube2w | ubiquitin-conjugating enzym... | XM_017322048.1 | 47.1% | 40.5% | (many diffs) |
| 14 | mouse | 66799 | Ube2w | ubiquitin-conjugating enzym... | NR_073122.1 | 10.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 543
- ORF length:
- 477
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gtcaatgcag aaacgactac agaaagaact gttGGCTTTG CAAAATGACC 121 CATCTCCTGG AATGACCTTA AATGAGAAGA GTGCTCAAAA TTCAATTACA CAGTGGATTG 181 TAGACATGGA AAGTGCACCA GGTACCTTAT ATGAAGGGGA AAAATTTCAA CTTCTATTTA 241 AATTTAGTAG TCGATATCCT TTTGACTCTC CTCAGGTCAT GTTTACTGGT GAAAATATTC 301 CTGTTCATCC TCATGTTTAT AGCAATGGTC ATATCTGTTT ATCCATTCTA ACAGAAGACT 361 GGTCCCCAGC GCTCTCAGTC CAATCAGTTT GTCTTAGCAT TATTAGCATG CTTTCCAGCT 421 GCAAGGAAAA GAGACGACCA CCGGATAATT CTTTTTATGT GCGAACATGT AACAAGAATC 481 CAAAGAAAAC AAAATGGTGG TATCATGAAC TGAAATCTGC CTTCATACTA TCTATTACGG 541 ATTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 601 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 661 GGCTTTATAT ATCTTGTGGA AAGGACGAAA CAAGGGTAGC CGCACGAGGC GTACGCGTTA 721 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt