Construct: ORF TRCN0000472973
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005934.1_s317c1
- Derived from:
- ccsbBroadEn_11298
- DNA Barcode:
- CGGAGCCCGGCTTCAGTTCATCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MBTPS1 (8720)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472973
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8720 | MBTPS1 | membrane bound transcriptio... | NM_003791.4 | 52.4% | 52.3% | 856A>G;1317C>T;1657_3156del |
| 2 | mouse | 56453 | Mbtps1 | membrane-bound transcriptio... | NM_001167910.1 | 46.8% | 50.2% | (many diffs) |
| 3 | mouse | 56453 | Mbtps1 | membrane-bound transcriptio... | NM_019709.4 | 46.8% | 50.2% | (many diffs) |
| 4 | mouse | 56453 | Mbtps1 | membrane-bound transcriptio... | XM_011248449.2 | 46.8% | 50.2% | (many diffs) |
| 5 | mouse | 56453 | Mbtps1 | membrane-bound transcriptio... | XM_011248450.2 | 46.8% | 50.2% | (many diffs) |
| 6 | mouse | 56453 | Mbtps1 | membrane-bound transcriptio... | XM_011248451.2 | 46.1% | 49.4% | (many diffs) |
| 7 | mouse | 56453 | Mbtps1 | membrane-bound transcriptio... | XR_878851.1 | 44% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1722
- ORF length:
- 1656
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gcttgtcaac atctggctgc ttctgctcgt ggttttgctc tgtgggaaga 121 aacatctggg cgacagactg gaaaagaaat cttttgaaaa ggccccatgc cctggctgtt 181 cccacctgac tttgaaggtg gaattctcat caacagttgt ggaatatgaa tatattgtgg 241 ctttcaatgg atactttaca gccaaagcta gaaattcatt tatttcaagt gccctgaaga 301 gcagtgaagt agacaattgg agaattatac ctcgaaacaa tccatccagt gactacccta 361 gtgattttga ggtgattcag ataaaagaaa aacagaaagc ggggctgcta acacttgaag 421 atcatccaaa catcaaacgg gtcacgcccc aacgaaaagt ctttcgttcc ctcaagtatg 481 ctgaatctga ccccacagta ccctgcaatg aaacccggtg gagccagaag tggcaatcat 541 cacgtcccct gcgaagagcc agcctctccc tgggctctgg cttctggcat gctacgggaa 601 ggcattcgag cagacggctg ctgagagcca tcccgcgcca ggttgcccag acactgcagg 661 cagatgtgct ctggcagatg ggatatacag gtgctaatgt aagagttgct gtttttgaca 721 ctgggctgag cgagaagcat ccccacttca aaaatgtgaa ggagagaacc aactggacca 781 acgagcgaac gctggacgat gggttgggcc atggcacatt cgtggcaggt gtgatagcca 841 gcatgaggga gtgccaagga tttgctccag atgcagaact tcacattttc agggtcttta 901 ccaataatca ggtatcttac gcatcttggt ttttggacgc cttcaactat gccattttaa 961 agaagatcga cgtgttaaac ctcagcatcg gcggcccgga cttcatggat catccgtttg 1021 ttgacaaggt gtgggaatta acagctaaca atgtaatcat ggtttctgct attggcaatg 1081 acggacctct ttatggcact ctgaataacc ctgctgatca aatggatgtg attggagtag 1141 gcggcattga ctttgaagat aacatcgccc gcttttcttc aaggggaatg actacctggg 1201 agctaccagg aggctacggt cgcatgaaac ctgacattgt cacctatggt gctggcgtgc 1261 ggggtTCTGG CGTGAAAGGG GGGTGCCGGG CCCTCTCAGG GACCAGTGTT GCTTCTCCAG 1321 TGGTTGCAGG TGCTGTCACC TTGTTAGTGA GCACAGTCCA GAAGCGTGAG CTGGTGAATC 1381 CTGCCAGTAT GAAGCAGGCC CTGATCGCGT CAGCCCGGAG GCTCCCCGGG GTCAACATGT 1441 TTGAGCAAGG CCACGGCAAG CTCGATCTGC TCAGAGCCTA TCAGATCCTC AACAGCTACA 1501 AGCCACAGGC AAGTTTGAGC CCCAGCTACA TAGATCTGAC TGAGTGTCCC TACATGTGGC 1561 CCTACTGCTC CCAGCCCATC TACTATGGAG GAATGCCGAC AGTTGTTAAT GTCACCATCC 1621 TCAACGGCAT GGGAGTCACA GGAAGAATTG TAGATAAGCC TGACTGGCAG CCCTATTTGC 1681 CACAGAACGG AGACAACATT GAAGTTGCCT TCTCCTACTC CTACCCAACT TTCTTGTACA 1741 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1801 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1861 AGGACGACGG AGCCCGGCTT CAGTTCATCG GACGCGTTAA GTCgacaatc aacctctgga 1921 ttacaaaatt tgtgaaagat t