Construct: ORF TRCN0000472981
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012410.1_s317c1
- Derived from:
- ccsbBroadEn_06638
- DNA Barcode:
- GTCCATCAAGGGCCAGGGATCTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NFYA (4800)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472981
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4800 | NFYA | nuclear transcription facto... | NM_021705.4 | 99.7% | 99.6% | 168A>G;730C>A |
| 2 | human | 4800 | NFYA | nuclear transcription facto... | NM_002505.5 | 91.4% | 91.3% | 76_162del;255A>G;817C>A |
| 3 | mouse | 18044 | Nfya | nuclear transcription facto... | NM_010913.2 | 90.6% | 99.3% | (many diffs) |
| 4 | mouse | 18044 | Nfya | nuclear transcription facto... | NM_001347402.1 | 88.7% | 97.4% | (many diffs) |
| 5 | mouse | 18044 | Nfya | nuclear transcription facto... | XM_006523793.1 | 83.5% | 91.5% | (many diffs) |
| 6 | mouse | 18044 | Nfya | nuclear transcription facto... | NM_001347401.1 | 83.3% | 91.3% | (many diffs) |
| 7 | mouse | 18044 | Nfya | nuclear transcription facto... | XM_017317292.1 | 83.3% | 91.3% | (many diffs) |
| 8 | mouse | 18044 | Nfya | nuclear transcription facto... | NM_001110832.1 | 83% | 91% | (many diffs) |
| 9 | mouse | 18044 | Nfya | nuclear transcription facto... | XM_006523795.1 | 81.8% | 89.8% | (many diffs) |
| 10 | mouse | 18044 | Nfya | nuclear transcription facto... | XM_006523794.1 | 81.3% | 89.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1020
- ORF length:
- 954
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gcagtataca gcaaacagca atagttcgac agagcagatt gttgtccagg 121 caggacagat tcagcagcag gtccaagggc agccattaat ggtgcaggtc agtggaggcc 181 agctaatcac atcaactggc caacccatca tggtccaggc tgtccctggt gggcaaggtc 241 aaaccatcat gcaagtacct gtttctggaa cacagggttt gcagcaaata cagttggtcc 301 cacctggaca gatccagatc cagggtggac aggctgtgca ggtgcagggc cagcagggcc 361 agacccagca gatcatcatc cagcagcccc agacggctgt cactgctggc cagactcaga 421 cacagcagca gattgctgtc cagggacagc aagtggcaca gactgctgaa gggcagacca 481 tcgtctatca accagttaat gcagatggca ccattctcca gcaagttaca gtccctgttt 541 caggcatgat cactatccca gcagccagtt tggcaggagc acagattgtt caaacaggag 601 ccaataccaa cacaaccagc agtgggcaag ggactgtcac tgtgaCACTA CCAGTGGCAG 661 GCAATGTGGT CAATTCAGGA GGGATGGTCA TGATGGTTCC TGGGGCTGGC TCTGTGCCTG 721 CTATCCAAAG AATCCCTCTA CCTGGAGCAG AGATGCTTGA AGAAGAGCCT CTCTACGTGA 781 ATGCCAAACA ATACAACCGT ATTCTTAAGA GGAGGCAAGC CCGAGCTAAA CTAGAGGCAG 841 AAGGGAAAAT TCCAAAGGAG AGAAGGAAAT ACCTGCATGA GTCTCGGCAC CGTCATGCCA 901 TGGCACGGAA GCGTGGTGAA GGTGGACGAT TTTTCTCTCC AAAGGAAAAG GATAGTCCCC 961 ATATGCAGGA TCCAAACCAA GCCGATGAAG AAGCAATGAC ACAGATCATC CGAGTGTCCT 1021 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1081 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1141 TTTATATATC TTGTGGAAAG GACGAGTCCA TCAAGGGCCA GGGATCTTCA CGCGTTAAGT 1201 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt