Construct: ORF TRCN0000472982
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011019.1_s317c1
- Derived from:
- ccsbBroadEn_06860
- DNA Barcode:
- TCACCCTCTAACTAAACGATACGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RGR (5995)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472982
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5995 | RGR | retinal G protein coupled r... | NM_002921.3 | 99.6% | 100% | 19C>T;27T>C;471C>T |
| 2 | human | 5995 | RGR | retinal G protein coupled r... | NM_001012720.2 | 98.3% | 98.6% | (many diffs) |
| 3 | human | 5995 | RGR | retinal G protein coupled r... | NM_001012722.1 | 85.4% | 85.7% | (many diffs) |
| 4 | human | 5995 | RGR | retinal G protein coupled r... | XM_024448118.1 | 71.5% | 71.1% | (many diffs) |
| 5 | human | 5995 | RGR | retinal G protein coupled r... | XR_002957005.1 | 30.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 951
- ORF length:
- 885
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agagaccagt gccttgccca ccggcttcgg ggagctcgag gtgctggctg 121 tggggatggt gctactggtg gaagctctct ccggtctcag cctcaatacc ctgaccatct 181 tctctttctg caagaccccg gagctgcgga ctccctgcca cctactggtg ctgagcttgg 241 ctcttgcgga cagtgggatc agcctgaatg ccctcgttgc agccacatcc agccttctcc 301 gtgtctccca caggcgctgg ccctacggct cggacggctg ccaggctcac ggcttccagg 361 gctttgtgac agcgttggcc agcatctgca gcagtgcagc catcgcatgg gggcgttatc 421 accactactg cacccgtagc cagctggcct ggaactcagc cgtctctctg gtgctcttcg 481 tgtggctgtc ttctgccttc tgggcagctc tgccccttct gggttggggt cactatgact 541 atgagccact GGGGACATGC TGCACCCTGG ACTACTCCAA GGGGGACAGA AACTTCACCA 601 GCTTCCTCTT CACCATGTCC TTCTTCAACT TCGCCATGCC CCTCTTCATC ACGATCACTT 661 CCTACAGTCT CATGGAGCAG AAACTGGGGA AGAGTGGCCA TCTCCAGGTA AACACCACTC 721 TGCCAGCAAG GACGCTGCTG CTCGGCTGGG GCCCCTATGC CATCCTGTAT CTATACGCAG 781 TCATCGCAGA CGTGACTTCC ATCTCCCCCA AACTGCAGAT GGTGCCCGCC CTCATTGCCA 841 AAATGGTGCC CACGATCAAT GCCATCAACT ATGCCCTGGG CAATGAGATG GTCTGCAGGG 901 GAATCTGGCA GTGCCTCTCA CCGCAGAAGA GGGAGAAGGA CCGAACCAAG TGCCCAACTT 961 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1021 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1081 CTTGTGGAAA GGACGATCAC CCTCTAACTA AACGATACGA ACGCGTTAAG TCgacaatca 1141 acctctggat tacaaaattt gtgaaagatt