Construct: ORF TRCN0000473000
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009786.1_s317c1
- Derived from:
- ccsbBroadEn_04457
- DNA Barcode:
- ATTATAAGGAATGTCTACAAAAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TOX2 (84969)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473000
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84969 | TOX2 | TOX high mobility group box... | NM_001098796.2 | 100% | 100% | |
| 2 | human | 84969 | TOX2 | TOX high mobility group box... | NM_032883.3 | 100% | 100% | |
| 3 | human | 84969 | TOX2 | TOX high mobility group box... | NM_001098797.2 | 91.6% | 91.6% | 1_126del |
| 4 | human | 84969 | TOX2 | TOX high mobility group box... | XM_006723884.1 | 90% | 90% | 1_153del |
| 5 | human | 84969 | TOX2 | TOX high mobility group box... | NM_001098798.2 | 84.8% | 84.8% | 1_153del;904_905ins81 |
| 6 | human | 84969 | TOX2 | TOX high mobility group box... | XM_017028109.1 | 65.7% | 65.7% | 750_751ins477 |
| 7 | human | 84969 | TOX2 | TOX high mobility group box... | XR_001754425.1 | 50.8% | 1_27del;1060_1061ins197;1223_2155del | |
| 8 | mouse | 269389 | Tox2 | TOX high mobility group box... | XM_017318743.1 | 89% | 93.1% | (many diffs) |
| 9 | mouse | 269389 | Tox2 | TOX high mobility group box... | XM_006499614.3 | 86% | 89% | (many diffs) |
| 10 | mouse | 269389 | Tox2 | TOX high mobility group box... | XM_006499615.3 | 86% | 89% | (many diffs) |
| 11 | mouse | 269389 | Tox2 | TOX high mobility group box... | XM_006499612.3 | 81.6% | 85.3% | (many diffs) |
| 12 | mouse | 269389 | Tox2 | TOX high mobility group box... | XM_006499613.3 | 80.9% | 83.7% | (many diffs) |
| 13 | mouse | 269389 | Tox2 | TOX high mobility group box... | XM_006499608.3 | 80.5% | 84.2% | (many diffs) |
| 14 | mouse | 269389 | Tox2 | TOX high mobility group box... | XM_006499611.3 | 79% | 81.8% | (many diffs) |
| 15 | mouse | 269389 | Tox2 | TOX high mobility group box... | XM_006499610.3 | 78.4% | 81.1% | (many diffs) |
| 16 | mouse | 269389 | Tox2 | TOX high mobility group box... | NM_001098799.1 | 78% | 80.7% | (many diffs) |
| 17 | mouse | 269389 | Tox2 | TOX high mobility group box... | XM_006499609.3 | 75.2% | 77.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1458
- ORF length:
- 1392
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag tgacggaaac ccagagctcc tgtcaaccag ccagacctac aacggccaga 121 gcgagaacaa cgaagactat gagatccccc cgataacacc tcccaacctc ccggagccat 181 ccctcctgca cctgggggac cacgaagcca gctaccactc gctgtgccac ggcctcaccc 241 ccaacggtct gctccctgcc tactcctatc aggccatgga cctcccagcc atcatggtgt 301 ccaacatgct agcacaggac agccacctgc tgtcgggcca gctgcccacg atccaggaga 361 tggtccactc ggaagtggct gcctatgact cgggccggcc cgggcccctg ctgggtcgcc 421 cggcaatgct ggccagccac atgagtgccc tcagccagtc ccagctcatc tcgcagatgg 481 gcatccggag cagcatcgcc cacagctccc catcaccgcc ggggagcaag tcagcgaccc 541 cctctccctc cagctccact caggaagagg agtcggaagt gcatttcaag atctcgggag 601 aaaagagacc ttcagccgac ccaggaaaaa aggccaagaa cccgaagaag aagaaaaaga 661 aggaccccaa tgagccgcag aagcctgtgt cggcctacgc actcttcttc agagacactc 721 aggccgccat caagggtcag aaccccagtg ccactttcgg tgacgtgtcc aaaatcgtgg 781 cctccatgtg ggacagcctg ggagaggaac agaagcaggc ctacaagagg aagacagaag 841 cagcaaagaa ggaatatctg aaggccctgg cagcctaccg ggctagcctc gtctccaaga 901 gctccccaga tcaaggtgag accaagagca ctcaggcaaa cccaccagcc aaaatgctcc 961 cacccaagca gcccatgtat gccatgccag gcctggcctc cttcctgacg ccgtcggacc 1021 tgcaggcctt ccgcagtggg gcctcccctg ccagcctcgc ccggacgctg ggctccaagt 1081 ctctgctgcc aggcctcagt gcgtccccgc cgccgccacc cTCCTTCCCG CTCAGCCCCA 1141 CACTGCACCA GCAGCTGTCA CTGCCCCCTC ACGCCCAGGG CGCCCTCCTC AGTCCACCTG 1201 TTAGCATGTC CCCAGCCCCC CAGCCCCCTG TCCTGCCCAC CCCCATGGCA CTCCAGGTGC 1261 AGCTGGCGAT GAGCCCCTCA CCTCCAGGGC CACAGGACTT CCCGCACATC TCTGAGTTCC 1321 CCAGCAGCTC GGGATCCTGC TCACCTGGCC CATCCAACCC CACCAGCAGC GGGGACTGGG 1381 ACAGCAGCTA CCCCAGTGGG GAGTGTGGCA TCAGCACCTG CAGCCTGCTC CCCAGGGACA 1441 AATCGCTCTA CCTCACCTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1501 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1561 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAATTATAA GGAATGTCTA 1621 CAAAAGGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt