Construct: ORF TRCN0000473001

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011231.1_s317c1
Derived from:
ccsbBroadEn_03568
DNA Barcode:
AGCTACAACACGCCTGGGAGTTGC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TMEM143 (55260)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000473001
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55260 TMEM143 transmembrane protein 143 NM_018273.4 99.9% 100% 1183C>A
2 human 55260 TMEM143 transmembrane protein 143 NM_001303538.2 92.3% 92.3% 261_262ins105;1078C>A
3 human 55260 TMEM143 transmembrane protein 143 NM_001303539.2 85.7% 85.8% 366_367ins195;988C>A
4 human 55260 TMEM143 transmembrane protein 143 NM_001303540.2 78.1% 78.2% 261_262ins300;883C>A
5 human 55260 TMEM143 transmembrane protein 143 NR_130317.2 46.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1443
ORF length:
1377
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgac agtcgagctt tggctaaggc tccggggaaa gggtctagcc atgctgcatg
121tgacccgggg ggtctggggg tccagggtcc gagtatggcc actgttgccc gcgctcctcg
181ggcccccccg ggccctctca tcgctggcag ccaaaatggg ggagtatcgc aagatgtgga
241accccaggga gccccgcgac tgggcccagc agtaccgcga gcgcttcatt cccttctcca
301aggagcagct gctccgcctc ctaatacagg aattccactc gagtccggca gagaaggcgg
361ctttggaggc gttctcggcc cacgtggact tctgcaccct gttccactac caccaaatcc
421tggcccggct gcaggcctta tatgacccca tcaaccctga cagggagacc ctcgatcagc
481catcactaac ggatccccag cgtctgtcta atgagcagga ggtgcttcgg gctctggagc
541ccctgctggc ccaggccaac ttctccccgc tgtctgagga caccctggcc tacgcgctgg
601tggtccacca ccctcaggat gaggtccagg tgacagtaaa tttggatcag tatgtctaca
661ttcacttctg ggccctgggc cagcgagtcg ggcagatgcc cctgaagtcc agcgtgggct
721ccaggcgtgg cttcttcacc aagctgcccc ctgcggagag gagatacttt aagcgggtgg
781tcctggcagc ccggaccaaa cggggacacc tggtactgaa gagtttcaag gacacgccgc
841tggaaggcct ggagcagctg ctgccggagc tgaaggtgcg cacgcccacc ctgcagcgcg
901ccctgctcaa cctcatgctg gtagtctccg gcgtggcgat cttcgtcaac gtgggcatgg
961tggtgctaac cgacctcaag gtggccacct ccctgctgct gctgctcttc gccatcttca
1021tgggcctgcg ggcctcCAAG ATGTTCGGGC AGCGGCGCAG CGCGCAGGCG TTGGAGCTGG
1081CGCACATGCT GTACTATCGC AGTACGTCCA ACAACTCGGA GCTGCTCAGC GCCCTGGCCC
1141TGCGCGCGCA GGACGAGCAC ACCAAGGAGG CGCTGCTGGC TCACAGCTTC CTGGCCCGGC
1201GGCCAGGGGG CACTCAAGGC TCGCCCGAAG AGACCTCCAG GTGGCTCAGG TCGGAGGTGG
1261AGAACTGGCT CCTAGCCAAG TCAGGCTGTG AGGTGACCTT CAACGGAACT CGGGCCCTGG
1321CGCATCTGCA GGCCCTGACC CCCAGCATGG GATTGTACCC ACCCCCGGGT TTCCCCAAAC
1381TAGACCCAGT GGCTCCGATC ACTTCCGAGC CCCCGCAAGC CACGCCCAGC AGTAACATCT
1441CCTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC
1501TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT
1561GGCTTTATAT ATCTTGTGGA AAGGACGAAG CTACAACACG CCTGGGAGTT GCACGCGTTA
1621AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt

Download FASTA (ORF) (Full)