Construct: ORF TRCN0000473001
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011231.1_s317c1
- Derived from:
- ccsbBroadEn_03568
- DNA Barcode:
- AGCTACAACACGCCTGGGAGTTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM143 (55260)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473001
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55260 | TMEM143 | transmembrane protein 143 | NM_018273.4 | 99.9% | 100% | 1183C>A |
| 2 | human | 55260 | TMEM143 | transmembrane protein 143 | NM_001303538.2 | 92.3% | 92.3% | 261_262ins105;1078C>A |
| 3 | human | 55260 | TMEM143 | transmembrane protein 143 | NM_001303539.2 | 85.7% | 85.8% | 366_367ins195;988C>A |
| 4 | human | 55260 | TMEM143 | transmembrane protein 143 | NM_001303540.2 | 78.1% | 78.2% | 261_262ins300;883C>A |
| 5 | human | 55260 | TMEM143 | transmembrane protein 143 | NR_130317.2 | 46.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1443
- ORF length:
- 1377
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac agtcgagctt tggctaaggc tccggggaaa gggtctagcc atgctgcatg 121 tgacccgggg ggtctggggg tccagggtcc gagtatggcc actgttgccc gcgctcctcg 181 ggcccccccg ggccctctca tcgctggcag ccaaaatggg ggagtatcgc aagatgtgga 241 accccaggga gccccgcgac tgggcccagc agtaccgcga gcgcttcatt cccttctcca 301 aggagcagct gctccgcctc ctaatacagg aattccactc gagtccggca gagaaggcgg 361 ctttggaggc gttctcggcc cacgtggact tctgcaccct gttccactac caccaaatcc 421 tggcccggct gcaggcctta tatgacccca tcaaccctga cagggagacc ctcgatcagc 481 catcactaac ggatccccag cgtctgtcta atgagcagga ggtgcttcgg gctctggagc 541 ccctgctggc ccaggccaac ttctccccgc tgtctgagga caccctggcc tacgcgctgg 601 tggtccacca ccctcaggat gaggtccagg tgacagtaaa tttggatcag tatgtctaca 661 ttcacttctg ggccctgggc cagcgagtcg ggcagatgcc cctgaagtcc agcgtgggct 721 ccaggcgtgg cttcttcacc aagctgcccc ctgcggagag gagatacttt aagcgggtgg 781 tcctggcagc ccggaccaaa cggggacacc tggtactgaa gagtttcaag gacacgccgc 841 tggaaggcct ggagcagctg ctgccggagc tgaaggtgcg cacgcccacc ctgcagcgcg 901 ccctgctcaa cctcatgctg gtagtctccg gcgtggcgat cttcgtcaac gtgggcatgg 961 tggtgctaac cgacctcaag gtggccacct ccctgctgct gctgctcttc gccatcttca 1021 tgggcctgcg ggcctcCAAG ATGTTCGGGC AGCGGCGCAG CGCGCAGGCG TTGGAGCTGG 1081 CGCACATGCT GTACTATCGC AGTACGTCCA ACAACTCGGA GCTGCTCAGC GCCCTGGCCC 1141 TGCGCGCGCA GGACGAGCAC ACCAAGGAGG CGCTGCTGGC TCACAGCTTC CTGGCCCGGC 1201 GGCCAGGGGG CACTCAAGGC TCGCCCGAAG AGACCTCCAG GTGGCTCAGG TCGGAGGTGG 1261 AGAACTGGCT CCTAGCCAAG TCAGGCTGTG AGGTGACCTT CAACGGAACT CGGGCCCTGG 1321 CGCATCTGCA GGCCCTGACC CCCAGCATGG GATTGTACCC ACCCCCGGGT TTCCCCAAAC 1381 TAGACCCAGT GGCTCCGATC ACTTCCGAGC CCCCGCAAGC CACGCCCAGC AGTAACATCT 1441 CCTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1501 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1561 GGCTTTATAT ATCTTGTGGA AAGGACGAAG CTACAACACG CCTGGGAGTT GCACGCGTTA 1621 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt