Transcript: Human NM_018273.4

Homo sapiens transmembrane protein 143 (TMEM143), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-05-02
Taxon:
Homo sapiens (human)
Gene:
TMEM143 (55260)
Length:
2263
CDS:
21..1400

Additional Resources:

NCBI RefSeq record:
NM_018273.4
NBCI Gene record:
TMEM143 (55260)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_018273.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000233173 TGGCGCACATGCTGTACTATC pLKO_005 1033 CDS 100% 10.800 15.120 N TMEM143 n/a
2 TRCN0000163064 CCTGGTACTGAAGAGTTTCAA pLKO.1 764 CDS 100% 5.625 7.875 N TMEM143 n/a
3 TRCN0000166539 CTATCGCAGTACGTCCAACAA pLKO.1 1049 CDS 100% 4.950 6.930 N TMEM143 n/a
4 TRCN0000165629 GCTAGGTAGGTGAACCAGAAA pLKO.1 2044 3UTR 100% 4.950 6.930 N TMEM143 n/a
5 TRCN0000166092 GAGGAGATACTTTAAGCGGGT pLKO.1 713 CDS 100% 0.540 0.756 N TMEM143 n/a
6 TRCN0000166540 CCGCCAGGTAGAAGAGTTAAA pLKO.1 1741 3UTR 100% 13.200 9.240 N TMEM143 n/a
7 TRCN0000233172 CTGCGGAGAGGAGATACTTTA pLKO_005 706 CDS 100% 13.200 9.240 N TMEM143 n/a
8 TRCN0000166091 GAGGTCCAGGTGACAGTAAAT pLKO.1 576 CDS 100% 13.200 9.240 N TMEM143 n/a
9 TRCN0000233171 CAGTAAATTTGGATCAGTATG pLKO_005 589 CDS 100% 10.800 7.560 N TMEM143 n/a
10 TRCN0000233170 CCTCGATCAGCCATCACTAAC pLKO_005 425 CDS 100% 10.800 7.560 N TMEM143 n/a
11 TRCN0000164843 CTGTTCCACTACCACCAAATC pLKO.1 354 CDS 100% 10.800 7.560 N TMEM143 n/a
12 TRCN0000163844 CTGGTACTGAAGAGTTTCAAG pLKO.1 765 CDS 100% 4.950 3.465 N TMEM143 n/a
13 TRCN0000165088 GACACCTGGTACTGAAGAGTT pLKO.1 760 CDS 100% 4.950 3.465 N TMEM143 n/a
14 TRCN0000165396 CTTCATTCCCTTCTCCAAGGA pLKO.1 239 CDS 100% 2.640 1.848 N TMEM143 n/a
15 TRCN0000233174 AGTCCCACCCTCACCATAAAT pLKO_005 1648 3UTR 100% 15.000 10.500 N TMEM143 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_018273.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03568 pDONR223 100% 99.9% 100% None 1183C>A n/a
2 ccsbBroad304_03568 pLX_304 0% 99.9% 100% V5 1183C>A n/a
3 TRCN0000473001 AGCTACAACACGCCTGGGAGTTGC pLX_317 29.8% 99.9% 100% V5 1183C>A n/a
Download CSV