Construct: ORF TRCN0000473011
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008410.1_s317c1
- Derived from:
- ccsbBroadEn_11104
- DNA Barcode:
- ATGTTATTTAATGTTTCATATAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPL5 (6125)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473011
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6125 | RPL5 | ribosomal protein L5 | NM_000969.5 | 34% | 32.9% | 292_361del;374_891del |
| 2 | human | 6125 | RPL5 | ribosomal protein L5 | NR_146333.1 | 29.5% | 1_129del;433_1024del | |
| 3 | mouse | 100503670 | Rpl5 | ribosomal protein L5 | XM_006534684.2 | 30.5% | 32.7% | (many diffs) |
| 4 | mouse | 100503670 | Rpl5 | ribosomal protein L5 | NM_016980.2 | 30.4% | 32.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 204
- ORF end:
- 507
- ORF length:
- 303
- Sequence:
-
1 GGAGTTTCCC CACACTGAGT GGGTGGAGAC TGAAGTTAGG CCAGCTTGGC ACTTGATGTA 61 ATTCTCCTTG GAATTTGCCC TTTTTGAGTT TGGATCTTGG TTCATTCTCA AGCCTCAGAC 121 AGTGGTTCAA AGTTTTTTTC TTCCATTTCA GGTGTCGTGA GGCTAGCATC GATTGATCAA 181 CAAGTTTGTA CAAAAAAGTT GGCATGGGGT TTGTTAAAGT TGTTAAGAAT AAGGCCTACT 241 TTAAGAGATA CCAAGTGAAA TTTAGAAGAC GACGAGAGGG TAAAACTGAT TATTATGCTC 301 GGAAACGCTT GGTGATACAA GATAAAAATA AATACAACAC ACCCAAATAC AGGATGATAG 361 TTCGTGTGAC AAACAGAGAT ATCATTTGTC AGATTGCTTA TGCCCGTATA GAGGGGGATA 421 TGATAGTCTG CGCAGCGTAT GCACACGAAC TGCCAAAATA TGGTGTGAAG GTTGGCCTGA 481 CAAATTATGC TGCAGCCAAG TGGAGGTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 541 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 601 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAATGTTATT 661 TAATGTTTCA TATAGGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 721 aagatt