Construct: ORF TRCN0000473017
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008226.1_s317c1
- Derived from:
- ccsbBroadEn_11163
- DNA Barcode:
- AAGAACATCCCGGTTGACTCGATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STK4 (6789)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473017
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6789 | STK4 | serine/threonine kinase 4 | NR_147975.2 | 12.5% | 1_57del;175_935del | |
| 2 | human | 6789 | STK4 | serine/threonine kinase 4 | XM_017028033.2 | 10% | 10% | 118_1164del |
| 3 | human | 6789 | STK4 | serine/threonine kinase 4 | XM_011529020.2 | 8.5% | 7.1% | (many diffs) |
| 4 | human | 6789 | STK4 | serine/threonine kinase 4 | NM_001352385.2 | 8.4% | 8.4% | 118_1386del |
| 5 | human | 6789 | STK4 | serine/threonine kinase 4 | NM_006282.5 | 8% | 8% | 118_1461del |
| 6 | human | 6789 | STK4 | serine/threonine kinase 4 | XM_005260531.3 | 7.2% | 6% | (many diffs) |
| 7 | human | 6789 | STK4 | serine/threonine kinase 4 | XM_005260530.2 | 6.8% | 5.7% | (many diffs) |
| 8 | human | 6789 | STK4 | serine/threonine kinase 4 | NR_147974.2 | 6.1% | 1_57del;174_1891delinsG | |
| 9 | mouse | 58231 | Stk4 | serine/threonine kinase 4 | XM_006499964.3 | 10.3% | 11.4% | (many diffs) |
| 10 | mouse | 58231 | Stk4 | serine/threonine kinase 4 | XM_006499963.3 | 8.1% | 9% | (many diffs) |
| 11 | mouse | 58231 | Stk4 | serine/threonine kinase 4 | NM_021420.3 | 7.2% | 8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 331
- ORF end:
- 448
- ORF length:
- 117
- Sequence:
-
1 CCTTTCCGTC CTCAGCCGTC GCTTCATGTG ACTCCACGGA GTACCGGGCG CCGTCCAGGC 61 ACCTCGATTA GTTCTCGAGC TTTTGGAGTA CGTCGTCTTT AGGTTGGGGG GAGGGGTTTT 121 ATGCGATGGA GTTTCCCCAC ACTGAGTGGG TGGAGACTGA AGTTAGGCCA GCTTGGCACT 181 TGATGTAATT CTCCTTGGAA TTTGCCCTTT TTGAGTTTGG ATCTTGGTTC ATTCTCAAGC 241 CTCAGACAGT GGTTCAAAGT TTTTTTCTTC CATTTCAGGT GTCGTGAGGC TAGCATCGAT 301 TGATCAACAA GTTTGTACAA AAAAGTTGGC ATGGAGACGG TACAGCTGAG GAACCCGCCG 361 CGCCGGCAGC TGAAAAAGTT GGATGAAGAT AGTTTAACCA AACAACCAGA AGAAGTATTT 421 GATGTCTTAG AGAAACTTGG AGAAGGGTGC CCAACTTTCT TGTACAAAGT GGTTGATATC 481 GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG 541 TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAAAGAACA 601 TCCCGGTTGA CTCGATTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg 661 aaagatt