Construct: ORF TRCN0000473021
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007761.1_s317c1
- Derived from:
- ccsbBroadEn_03173
- DNA Barcode:
- CGACTCCACAACAGGGAATAATTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMED7 (51014)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473021
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51014 | TMED7 | transmembrane p24 trafficki... | NM_181836.6 | 100% | 100% | |
| 2 | human | 100302736 | TMED7-TICAM2 | TMED7-TICAM2 readthrough | NM_001164469.3 | 83.9% | 83.9% | 564_565ins108 |
| 3 | human | 100302736 | TMED7-TICAM2 | TMED7-TICAM2 readthrough | NM_001164468.4 | 53.5% | 47.7% | (many diffs) |
| 4 | mouse | 66676 | Tmed7 | transmembrane p24 trafficki... | NM_025698.1 | 90.3% | 95.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 738
- ORF length:
- 672
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc gcggccgggg tccgcgcagc gctgggcggc cgtcgcgggc cgttgggggt 121 gcaggctgct cgcactgctg ctactggtgc ctggacccgg cggcgcctct gagatcacct 181 tcgagcttcc tgacaacgcc aagcagtgct tcTACGAGGA CATCGCTCAG GGCACCAAGT 241 GCACCCTGGA GTTCCAGGTG ATTACTGGTG GTCACTATGA TGTAGATTGT CGATTAGAAG 301 ATCCTGATGG TAAAGTGTTA TACAAAGAGA TGAAGAAACA GTATGATAGT TTTACCTTCA 361 CAGCCTCCAA AAATGGGACA TACAAATTTT GCTTCAGCAA TGAATTTTCT ACTTTCACAC 421 ATAAAACTGT ATATTTTGAT TTTCAAGTTG GAGAAGACCC ACCTTTGTTT CCTAGTGAGA 481 ACCGAGTCAG TGCTCTTACC CAGATGGAAT CTGCCTGTGT TTCAATTCAC GAAGCTCTGA 541 AGTCTGTCAT CGATTATCAG ACTCATTTCC GTTTAAGAGA AGCTCAAGGC CGAAGCCGAG 601 CAGAGGATCT AAATACAAGA GTGGCCTATT GGTCAGTAGG AGAAGCCCTC ATTCTTCTGG 661 TGGTTAGCAT AGGGCAGGTA TTTCTTTTGA AAAGCTTTTT CTCAGATAAA AGAACCACCA 721 CAACTCGTGT TGGATCATAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 781 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 841 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACGACTCC ACAACAGGGA 901 ATAATTTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt