Construct: ORF TRCN0000473025
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013100.1_s317c1
- Derived from:
- ccsbBroadEn_11204
- DNA Barcode:
- TACGCAACTGGGAAACCCCCGGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRPS1 (7227)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473025
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7227 | TRPS1 | transcriptional repressor G... | NM_001330599.2 | 66.5% | 66.5% | 918T>C;1035C>T;2560_3843del |
| 2 | human | 7227 | TRPS1 | transcriptional repressor G... | XM_011517268.2 | 66.5% | 66.5% | 918T>C;1035C>T;2560_3843del |
| 3 | human | 7227 | TRPS1 | transcriptional repressor G... | NM_001282902.3 | 66.3% | 66.3% | (many diffs) |
| 4 | human | 7227 | TRPS1 | transcriptional repressor G... | NM_001282903.3 | 66.2% | 66.2% | (many diffs) |
| 5 | human | 7227 | TRPS1 | transcriptional repressor G... | XM_011517267.1 | 66.2% | 66.2% | (many diffs) |
| 6 | human | 7227 | TRPS1 | transcriptional repressor G... | NM_014112.5 | 65.8% | 65.9% | (many diffs) |
| 7 | human | 7227 | TRPS1 | transcriptional repressor G... | XM_006716625.1 | 65.8% | 65.9% | (many diffs) |
| 8 | human | 7227 | TRPS1 | transcriptional repressor G... | XM_011517264.2 | 65.8% | 65.9% | (many diffs) |
| 9 | human | 7227 | TRPS1 | transcriptional repressor G... | XM_011517266.3 | 65.8% | 65.9% | (many diffs) |
| 10 | mouse | 83925 | Trps1 | transcriptional repressor G... | NM_032000.2 | 59.9% | 60.8% | (many diffs) |
| 11 | mouse | 83925 | Trps1 | transcriptional repressor G... | XM_030248827.1 | 59.9% | 60.8% | (many diffs) |
| 12 | mouse | 83925 | Trps1 | transcriptional repressor G... | NM_001310481.1 | 59.7% | 60.7% | (many diffs) |
| 13 | mouse | 83925 | Trps1 | transcriptional repressor G... | XM_011245776.3 | 59.4% | 60.3% | (many diffs) |
| 14 | mouse | 83925 | Trps1 | transcriptional repressor G... | XM_011245777.2 | 59.4% | 60.3% | (many diffs) |
| 15 | mouse | 83925 | Trps1 | transcriptional repressor G... | XM_011245778.3 | 59.4% | 60.3% | (many diffs) |
| 16 | mouse | 83925 | Trps1 | transcriptional repressor G... | XM_011245780.3 | 59.4% | 60.3% | (many diffs) |
| 17 | mouse | 83925 | Trps1 | transcriptional repressor G... | XM_030248826.1 | 59.4% | 60.3% | (many diffs) |
| 18 | mouse | 83925 | Trps1 | transcriptional repressor G... | XM_006521560.3 | 59.2% | 60.1% | (many diffs) |
| 19 | mouse | 83925 | Trps1 | transcriptional repressor G... | XM_006521563.3 | 45.3% | 45.9% | (many diffs) |
| 20 | mouse | 83925 | Trps1 | transcriptional repressor G... | XM_030248828.1 | 45% | 45.7% | (many diffs) |
| 21 | mouse | 83925 | Trps1 | transcriptional repressor G... | NM_001310485.1 | 44.9% | 45.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2628
- ORF length:
- 2559
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtccggaaa aagaaccccc ctctgagaaa cgttgcaagt gaaggcgagg 121 gccagatcct ggagcctata ggtacagaaa gcaaggtatc tggaaagaac aaagaatttt 181 ctgcagatca gatgtcagaa aatacggatc agagtgatgc tgcagaacta aatcataagg 241 aggaacatag cttgcatgtt caagatccat cttctagcag taagaaggac ttgaaaagcg 301 cagttctgag tgagaaggct ggcttcaatt atgaaagccc cagtaaggga ggaaactttc 361 cctcctttcc gcatgatgag gtgacagaca gaaatatgtt ggctttctca tctccagctg 421 ctgggggagt ctgtgagccc ttgaagtctc cgcaaagagc agaggcagat gaccctcaag 481 atatggcctg caccccctca ggggactcac tggagacaaa ggaagatcag aagatgtcac 541 caaaggctac agaggaaaca gggcaagcac agagtggtca agccaattgt caaggtttga 601 gcccagtttc agtggcctca aaaaacccac aagtgccttc agatgggggt gtaagactga 661 ataaatccaa aactgactta ctggtgaatg acaacccaga cccggcacct ctgtctccag 721 agcttcagga ctttaaatgc aatatctgtg gatatggtta ctacggcaac gaccccacag 781 atctgattaa gcacttccga aagtatcact taggactgca taaccgcacc aggcaagatg 841 ctgagctgga cagcaaaatc ttggcccttc ataacatggt gcagttcagc cattccaaag 901 acttccagaa ggtcaaccgt tctgtgtttt ctggtgtgct gcaggacatc aattcttcaa 961 ggcctgtttt actaaatggg acctacgatg tgcaggtgac ttcaggtgga acattcattg 1021 gcattggacg gaaaacacca gattgccaag ggaacaccaa gtatttccgc tgtaaattct 1081 gcaatttcac ttatatgggc aattcatcca ccgaattaga acaacatttt cttcagactc 1141 acccaaacaa aataaaagct tctctcccct cctctgaggt tgcaaaacct tcagagaaaa 1201 actctaacaa gtccatccct gcacttcaat ccagtgattc tggagacttg ggaaaatggc 1261 aggacaagat aacagtcaaa gcaggagatg acactcctgt tgggtactca gtgcccataa 1321 agcccctcga ttcctctaga caaaatggta cagaggccac cagttactac tggtgtaaat 1381 tttgtagttt cagctgtgag tcatctagct cacttaaact gctagaacat tatggcaagc 1441 agcacggagc agtgcagtca ggcggcctta atccagagtt aaatgataag ctttccaggg 1501 gctctgtcat taatcagaat gatctagcca aaagttcaga aggagagaca atgaccaaga 1561 cagacaagag ctcgagtggg gctaaaaaga aggacttctc cagcaaggga gccgaggata 1621 atatggtaac gagctataat tgtcagttct gtgacttccg atattccaaa agccatggcc 1681 ctgatgtaat tgtagtgggg ccacttctcc gtcattatca acagctccat aacattcaca 1741 agtgtaccat taaacactgt ccattctgtc ccagaggact ttgcagccca gaaaagcacc 1801 ttggagaaat tacttatccg tttgcttgta gaaaaagtaa ttgttcccac tgtgcactct 1861 tgcttctgca cttgtctcct ggggcggctg gaagctcgcg agtcaaacat cagtgccatc 1921 agtgttcatt caccacccct gacgtagatg tactcctctt tcactatgaa agtgtgcatg 1981 agtcccaagc atcggatgtc aaacaagaag caaatcacct gcaaggatcg gatgggcagc 2041 agtctgtcaa ggaaagcaaa gaacactcat gtaccaaatg tgatTTTATT ACCCAAGTGG 2101 AAGAAGAGAT TTCCCGACAC TACAGGAGAG CACACAGCTG CTACAAATGC CGTCAGTGCA 2161 GTTTTACAGC TGCCGATACT CAGTCACTAC TGGAGCACTT CAACACTGTT CACTGCCAGG 2221 AACAGGACAT CACTACAGCC AACGGCGAAG AGGACGGTCA TGCCATATCC ACCATCAAAG 2281 AGGAGCCCAA AATTGACTTC AGGGTCTACA ATCTGCTAAC TCCAGACTCT AAAATGGGAG 2341 AGCCAGTTTC TGAGAGTGTG GTGAAGAGAG AGAAGCTGGA AGAGAAGGAC GGGCTCAAAG 2401 AGAAAGTTTG GACCGAGAGT TCCAGTGATG ACCTTCGCAA TGTGACTTGG AGAGGGGCAG 2461 ACATCCTGCG GGGGAGTCCG TCATACACCC AAGCAAGCCT GGGGCTGCTG ACGCCTGTGT 2521 CTGGCACCCA AGAGCAGACA AAGACTCTAA GGGATAGTCC CAATGTGGAG GCCGCCCATC 2581 TGGCGCGACC TATTTATGGC TTGGCTGTGG AAACCAAGGG ATTCCTGTTG CCAACTTTCT 2641 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 2701 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 2761 GTGGAAAGGA CGATACGCAA CTGGGAAACC CCCGGTCACG CGTTAAGTCg acaatcaacc 2821 tctggattac aaaatttgtg aaagatt