Construct: ORF TRCN0000473025

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013100.1_s317c1
Derived from:
ccsbBroadEn_11204
DNA Barcode:
TACGCAACTGGGAAACCCCCGGTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TRPS1 (7227)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000473025
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7227 TRPS1 transcriptional repressor G... NM_001330599.2 66.5% 66.5% 918T>C;1035C>T;2560_3843del
2 human 7227 TRPS1 transcriptional repressor G... XM_011517268.2 66.5% 66.5% 918T>C;1035C>T;2560_3843del
3 human 7227 TRPS1 transcriptional repressor G... NM_001282902.3 66.3% 66.3% (many diffs)
4 human 7227 TRPS1 transcriptional repressor G... NM_001282903.3 66.2% 66.2% (many diffs)
5 human 7227 TRPS1 transcriptional repressor G... XM_011517267.1 66.2% 66.2% (many diffs)
6 human 7227 TRPS1 transcriptional repressor G... NM_014112.5 65.8% 65.9% (many diffs)
7 human 7227 TRPS1 transcriptional repressor G... XM_006716625.1 65.8% 65.9% (many diffs)
8 human 7227 TRPS1 transcriptional repressor G... XM_011517264.2 65.8% 65.9% (many diffs)
9 human 7227 TRPS1 transcriptional repressor G... XM_011517266.3 65.8% 65.9% (many diffs)
10 mouse 83925 Trps1 transcriptional repressor G... NM_032000.2 59.9% 60.8% (many diffs)
11 mouse 83925 Trps1 transcriptional repressor G... XM_030248827.1 59.9% 60.8% (many diffs)
12 mouse 83925 Trps1 transcriptional repressor G... NM_001310481.1 59.7% 60.7% (many diffs)
13 mouse 83925 Trps1 transcriptional repressor G... XM_011245776.3 59.4% 60.3% (many diffs)
14 mouse 83925 Trps1 transcriptional repressor G... XM_011245777.2 59.4% 60.3% (many diffs)
15 mouse 83925 Trps1 transcriptional repressor G... XM_011245778.3 59.4% 60.3% (many diffs)
16 mouse 83925 Trps1 transcriptional repressor G... XM_011245780.3 59.4% 60.3% (many diffs)
17 mouse 83925 Trps1 transcriptional repressor G... XM_030248826.1 59.4% 60.3% (many diffs)
18 mouse 83925 Trps1 transcriptional repressor G... XM_006521560.3 59.2% 60.1% (many diffs)
19 mouse 83925 Trps1 transcriptional repressor G... XM_006521563.3 45.3% 45.9% (many diffs)
20 mouse 83925 Trps1 transcriptional repressor G... XM_030248828.1 45% 45.7% (many diffs)
21 mouse 83925 Trps1 transcriptional repressor G... NM_001310485.1 44.9% 45.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2628
ORF length:
2559
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggtccggaaa aagaaccccc ctctgagaaa cgttgcaagt gaaggcgagg
121gccagatcct ggagcctata ggtacagaaa gcaaggtatc tggaaagaac aaagaatttt
181ctgcagatca gatgtcagaa aatacggatc agagtgatgc tgcagaacta aatcataagg
241aggaacatag cttgcatgtt caagatccat cttctagcag taagaaggac ttgaaaagcg
301cagttctgag tgagaaggct ggcttcaatt atgaaagccc cagtaaggga ggaaactttc
361cctcctttcc gcatgatgag gtgacagaca gaaatatgtt ggctttctca tctccagctg
421ctgggggagt ctgtgagccc ttgaagtctc cgcaaagagc agaggcagat gaccctcaag
481atatggcctg caccccctca ggggactcac tggagacaaa ggaagatcag aagatgtcac
541caaaggctac agaggaaaca gggcaagcac agagtggtca agccaattgt caaggtttga
601gcccagtttc agtggcctca aaaaacccac aagtgccttc agatgggggt gtaagactga
661ataaatccaa aactgactta ctggtgaatg acaacccaga cccggcacct ctgtctccag
721agcttcagga ctttaaatgc aatatctgtg gatatggtta ctacggcaac gaccccacag
781atctgattaa gcacttccga aagtatcact taggactgca taaccgcacc aggcaagatg
841ctgagctgga cagcaaaatc ttggcccttc ataacatggt gcagttcagc cattccaaag
901acttccagaa ggtcaaccgt tctgtgtttt ctggtgtgct gcaggacatc aattcttcaa
961ggcctgtttt actaaatggg acctacgatg tgcaggtgac ttcaggtgga acattcattg
1021gcattggacg gaaaacacca gattgccaag ggaacaccaa gtatttccgc tgtaaattct
1081gcaatttcac ttatatgggc aattcatcca ccgaattaga acaacatttt cttcagactc
1141acccaaacaa aataaaagct tctctcccct cctctgaggt tgcaaaacct tcagagaaaa
1201actctaacaa gtccatccct gcacttcaat ccagtgattc tggagacttg ggaaaatggc
1261aggacaagat aacagtcaaa gcaggagatg acactcctgt tgggtactca gtgcccataa
1321agcccctcga ttcctctaga caaaatggta cagaggccac cagttactac tggtgtaaat
1381tttgtagttt cagctgtgag tcatctagct cacttaaact gctagaacat tatggcaagc
1441agcacggagc agtgcagtca ggcggcctta atccagagtt aaatgataag ctttccaggg
1501gctctgtcat taatcagaat gatctagcca aaagttcaga aggagagaca atgaccaaga
1561cagacaagag ctcgagtggg gctaaaaaga aggacttctc cagcaaggga gccgaggata
1621atatggtaac gagctataat tgtcagttct gtgacttccg atattccaaa agccatggcc
1681ctgatgtaat tgtagtgggg ccacttctcc gtcattatca acagctccat aacattcaca
1741agtgtaccat taaacactgt ccattctgtc ccagaggact ttgcagccca gaaaagcacc
1801ttggagaaat tacttatccg tttgcttgta gaaaaagtaa ttgttcccac tgtgcactct
1861tgcttctgca cttgtctcct ggggcggctg gaagctcgcg agtcaaacat cagtgccatc
1921agtgttcatt caccacccct gacgtagatg tactcctctt tcactatgaa agtgtgcatg
1981agtcccaagc atcggatgtc aaacaagaag caaatcacct gcaaggatcg gatgggcagc
2041agtctgtcaa ggaaagcaaa gaacactcat gtaccaaatg tgatTTTATT ACCCAAGTGG
2101AAGAAGAGAT TTCCCGACAC TACAGGAGAG CACACAGCTG CTACAAATGC CGTCAGTGCA
2161GTTTTACAGC TGCCGATACT CAGTCACTAC TGGAGCACTT CAACACTGTT CACTGCCAGG
2221AACAGGACAT CACTACAGCC AACGGCGAAG AGGACGGTCA TGCCATATCC ACCATCAAAG
2281AGGAGCCCAA AATTGACTTC AGGGTCTACA ATCTGCTAAC TCCAGACTCT AAAATGGGAG
2341AGCCAGTTTC TGAGAGTGTG GTGAAGAGAG AGAAGCTGGA AGAGAAGGAC GGGCTCAAAG
2401AGAAAGTTTG GACCGAGAGT TCCAGTGATG ACCTTCGCAA TGTGACTTGG AGAGGGGCAG
2461ACATCCTGCG GGGGAGTCCG TCATACACCC AAGCAAGCCT GGGGCTGCTG ACGCCTGTGT
2521CTGGCACCCA AGAGCAGACA AAGACTCTAA GGGATAGTCC CAATGTGGAG GCCGCCCATC
2581TGGCGCGACC TATTTATGGC TTGGCTGTGG AAACCAAGGG ATTCCTGTTG CCAACTTTCT
2641TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA
2701CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT
2761GTGGAAAGGA CGATACGCAA CTGGGAAACC CCCGGTCACG CGTTAAGTCg acaatcaacc
2821tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)