Construct: ORF TRCN0000473029
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012733.1_s317c1
- Derived from:
- ccsbBroadEn_14965
- DNA Barcode:
- AGCAGGTATACTTCCTAATCGGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CRTAP (10491)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473029
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10491 | CRTAP | cartilage associated protein | NM_006371.5 | 99.7% | 99.7% | 411G>T;1032T>G;1044G>A |
| 2 | mouse | 56693 | Crtap | cartilage associated protein | NM_019922.2 | 86.1% | 89.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1269
- ORF length:
- 1203
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gccggggcgc cggggggccg cggcgctgct agcgctgctg tgcgtggcct 121 gcgcgctgcg cgccgggcgc gcccaatacg aacgctacag cttccgcagc ttcccacggg 181 acgagctgat gccgctcgag tcggcctacc ggcacgcgct ggacaagtac agcggcgagc 241 actgggccga gagcgtgggc tacctggaga tcagcctgcg gctgcaccgc ttgctgcgcg 301 acagcgaggc cttctgccac cgcaactgca gcgccgcgcc gcagcccgag cccgccgccg 361 gcctcgccag ctatcccgag ctgcgcctct tcgggggcct gctgcgccgc gcgcactgcc 421 tcaagcgctg caagcagggc ctgccagcct tccgccagtc ccagcccagc cgcgatgtgc 481 tggcggactt ccagcgccgc gagccctaca agttcctgca gttcgcttac ttcaaggcaa 541 ataatctccc caaagccatc gccgctgctc acacctttct actgaagcat cctgatgacg 601 aaatgatgaa gaggaacatg gcatattata agagcctgcc tggtgccgag gactacatta 661 aagacctgga aaccaagtca tatgaaagcc tgttcatccg agcagtgcgg gcatacaacg 721 gtgagaactg gagaacatcc atcacagaca tGGAGCTGGC CCTTCCCGAC TTCTTCAAAG 781 CCTTTTACGA GTGTCTCGCA GCCTGCGAGG GTTCCAGGGA GATCAAGGAC TTCAAGGATT 841 TCTACCTTTC CATAGCAGAT CATTATGTAG AAGTTCTGGA ATGCAAAATA CAGTGTGAAG 901 AGAACCTCAC CCCAGTTATA GGAGGCTATC CGGTTGAGAA ATTTGTGGCT ACCATGTATC 961 ATTACTTGCA GTTTGCCTAT TATAAGTTGA ACGACCTGAA GAATGCAGCC CCCTGTGCAG 1021 TCAGCTATCT GCTCTTTGAT CAGAATGACA AGGTCATGCA GCAGAACCTG GTGTATTACC 1081 AGTACCACAG GGACACGTGG GGCCTCTCAG ATGAGCACTT CCAGCCCAGA CCTGAAGCAG 1141 TTCAGTTCTT TAATGTGACC ACACTCCAGA AGGAGCTGTA TGACTTTGCT AAGGAAAATA 1201 TAATGGATGA TGATGAGGGA GAAGTTGTGG AATATGTGGA TGACCTCTTG GAACTGGAGG 1261 AGACCAGCTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1321 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1381 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAGCAGG TATACTTCCT AATCGGGTAC 1441 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt