Transcript: Human NM_006371.5

Homo sapiens cartilage associated protein (CRTAP), mRNA.

Source:
NCBI, updated 2019-09-15
Taxon:
Homo sapiens (human)
Gene:
CRTAP (10491)
Length:
6595
CDS:
65..1270

Additional Resources:

NCBI RefSeq record:
NM_006371.5
NBCI Gene record:
CRTAP (10491)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_006371.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000423588 GAGGCTATCCGGTTGAGAAAT pLKO_005 921 CDS 100% 13.200 18.480 N CRTAP n/a
2 TRCN0000421362 TCATGTTCACACCTATCTTTC pLKO_005 1461 3UTR 100% 10.800 15.120 N CRTAP n/a
3 TRCN0000179304 GCATCCTGATGACGAAATGAT pLKO.1 586 CDS 100% 5.625 7.875 N CRTAP n/a
4 TRCN0000181025 CCAATACGAACGCTACAGCTT pLKO.1 142 CDS 100% 2.640 3.696 N CRTAP n/a
5 TRCN0000147424 GACTACATTAAAGACCTGGAA pLKO.1 650 CDS 100% 2.640 3.696 N CRTAP n/a
6 TRCN0000146998 CGCTTACTTCAAGGCAAATAA pLKO.1 523 CDS 100% 15.000 10.500 N CRTAP n/a
7 TRCN0000419606 AGGGAGAAGTTGTGGAATATG pLKO_005 1215 CDS 100% 13.200 9.240 N CRTAP n/a
8 TRCN0000429901 CCTTTCCATAGCAGATCATTA pLKO_005 844 CDS 100% 13.200 9.240 N CRTAP n/a
9 TRCN0000412372 CTTTACTCTCCAAAGTGAAAG pLKO_005 1369 3UTR 100% 10.800 7.560 N CRTAP n/a
10 TRCN0000412545 GCGTTCAGGAAACACAGATTC pLKO_005 1300 3UTR 100% 10.800 7.560 N CRTAP n/a
11 TRCN0000178729 CGGTTGAGAAATTTGTGGCTA pLKO.1 930 CDS 100% 2.640 1.848 N CRTAP n/a
12 TRCN0000179830 CCTGAAGCAGTTCAGTTCTTT pLKO.1 1130 CDS 100% 0.563 0.394 N CRTAP n/a
13 TRCN0000147844 GTCATATGAAAGCCTGTTCAT pLKO.1 676 CDS 100% 0.000 0.000 N CRTAP n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_006371.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14965 pDONR223 96.5% 99.7% 99.7% None 411G>T;1032T>G;1044G>A n/a
2 ccsbBroad304_14965 pLX_304 0% 99.7% 99.7% V5 411G>T;1032T>G;1044G>A n/a
3 TRCN0000473029 AGCAGGTATACTTCCTAATCGGGT pLX_317 43.1% 99.7% 99.7% V5 411G>T;1032T>G;1044G>A n/a
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