Transcript: Mouse NM_019922.2

Mus musculus cartilage associated protein (Crtap), mRNA.

Source:
NCBI, updated 2017-05-21
Taxon:
Mus musculus (mouse)
Gene:
Crtap (56693)
Length:
1714
CDS:
114..1316

Additional Resources:

NCBI RefSeq record:
NM_019922.2
NBCI Gene record:
Crtap (56693)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_019922.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000182609 GCCCTACAAGTTTCTGCAGTT pLKO.1 548 CDS 100% 4.050 5.670 N Crtap n/a
2 TRCN0000345846 GCCCTACAAGTTTCTGCAGTT pLKO_005 548 CDS 100% 4.050 5.670 N Crtap n/a
3 TRCN0000177576 CACTATTTACAGTTTGCGTAT pLKO.1 1005 CDS 100% 4.050 3.240 N Crtap n/a
4 TRCN0000345848 CACTATTTACAGTTTGCGTAT pLKO_005 1005 CDS 100% 4.050 3.240 N Crtap n/a
5 TRCN0000182573 GCTCAGGAACACCTAATGGAT pLKO.1 1233 CDS 100% 3.000 2.400 N Crtap n/a
6 TRCN0000345850 GCTCAGGAACACCTAATGGAT pLKO_005 1233 CDS 100% 3.000 2.400 N Crtap n/a
7 TRCN0000197500 CGAAGCAGTTCAGTTCTTTAA pLKO.1 1178 CDS 100% 13.200 9.240 N Crtap n/a
8 TRCN0000216639 CAACAGAACCTGGTGTACTAT pLKO.1 1104 CDS 100% 5.625 3.938 N Crtap n/a
9 TRCN0000177082 CGAGATGATGAAGAGAAACAT pLKO.1 644 CDS 100% 5.625 3.938 N Crtap n/a
10 TRCN0000345847 CGAGATGATGAAGAGAAACAT pLKO_005 644 CDS 100% 5.625 3.938 N Crtap n/a
11 TRCN0000176863 GCGTATTACAAGTTGAATGAT pLKO.1 1020 CDS 100% 5.625 3.938 N Crtap n/a
12 TRCN0000345849 GCGTATTACAAGTTGAATGAT pLKO_005 1020 CDS 100% 5.625 3.938 N Crtap n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_019922.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14965 pDONR223 96.5% 86.1% 89.3% None (many diffs) n/a
2 ccsbBroad304_14965 pLX_304 0% 86.1% 89.3% V5 (many diffs) n/a
3 TRCN0000473029 AGCAGGTATACTTCCTAATCGGGT pLX_317 43.1% 86.1% 89.3% V5 (many diffs) n/a
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