Construct: ORF TRCN0000473031
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013840.1_s317c1
- Derived from:
- ccsbBroadEn_01563
- DNA Barcode:
- ACAGATCCTCGTATCTCAGAACGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SNCA (6622)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473031
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6622 | SNCA | synuclein alpha | NM_000345.4 | 100% | 100% | |
| 2 | human | 6622 | SNCA | synuclein alpha | NM_001146054.2 | 100% | 100% | |
| 3 | human | 6622 | SNCA | synuclein alpha | NM_001146055.2 | 100% | 100% | |
| 4 | human | 6622 | SNCA | synuclein alpha | XM_011532208.2 | 100% | 100% | |
| 5 | human | 6622 | SNCA | synuclein alpha | XM_017008562.1 | 100% | 100% | |
| 6 | human | 6622 | SNCA | synuclein alpha | XM_017008563.1 | 100% | 100% | |
| 7 | human | 6622 | SNCA | synuclein alpha | NM_007308.2 | 80% | 80% | 304_305ins84 |
| 8 | human | 6622 | SNCA | synuclein alpha | XM_011532203.1 | 71.2% | 52.2% | (many diffs) |
| 9 | human | 6622 | SNCA | synuclein alpha | XM_011532204.3 | 71.2% | 52.2% | (many diffs) |
| 10 | human | 6622 | SNCA | synuclein alpha | XM_011532205.2 | 71.2% | 52.2% | (many diffs) |
| 11 | human | 6622 | SNCA | synuclein alpha | XM_011532206.1 | 71.2% | 52.2% | (many diffs) |
| 12 | human | 6622 | SNCA | synuclein alpha | XM_011532207.1 | 71.2% | 52.2% | (many diffs) |
| 13 | mouse | 20617 | Snca | synuclein, alpha | NM_001042451.2 | 90.7% | 95% | (many diffs) |
| 14 | mouse | 20617 | Snca | synuclein, alpha | NM_009221.2 | 90.7% | 95% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 510
- ORF length:
- 420
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGATGTATT CATGAAAGGA CTTTCAAAGG 121 CCAAGGAGGG AGTTGTGGCT GCTGCTGAGA AAACCAAACA GGGTGTGGCA GAAGCAGCAG 181 GAAAGACAAA AGAGGGTGTT CTCTATGTAG GCTCCAAAAC CAAGGAGGGA GTGGTGCATG 241 GTGTGGCAAC AGTGGCTGAG AAGACCAAAG AGCAAGTGAC AAATGTTGGA GGAGCAGTGG 301 TGACGGGTGT GACAGCAGTA GCCCAGAAGA CAGTGGAGGG AGCAGGGAGC ATTGCAGCAG 361 CCACTGGCTT TGTCAAAAAG GACCAGTTGG GCAAGAATGA AGAAGGAGCC CCACAGGAAG 421 GAATTCTGGA AGATATGCCT GTGGATCCTG ACAATGAGGC TTATGAAATG CCTTCTGAGG 481 AAGGGTATCA AGACTACGAA CCTGAAGCCT ACCCAACTTT CTTGTACAAA GTGGTTGATA 541 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 601 CGTAACTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAACAGAT 661 CCTCGTATCT CAGAACGTAC GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt 721 gaaagatt