Construct: ORF TRCN0000473033
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010115.1_s317c1
- Derived from:
- ccsbBroadEn_02338
- DNA Barcode:
- TTTTATATCTCACATAGTTCCGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DHRS9 (10170)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473033
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10170 | DHRS9 | dehydrogenase/reductase 9 | NM_001142270.2 | 100% | 100% | |
| 2 | human | 10170 | DHRS9 | dehydrogenase/reductase 9 | NM_001142271.2 | 100% | 100% | |
| 3 | human | 10170 | DHRS9 | dehydrogenase/reductase 9 | NM_199204.2 | 100% | 100% | |
| 4 | human | 10170 | DHRS9 | dehydrogenase/reductase 9 | XM_011510475.1 | 100% | 100% | |
| 5 | human | 10170 | DHRS9 | dehydrogenase/reductase 9 | NM_001289763.2 | 84.1% | 84.1% | 1_180del |
| 6 | mouse | 241452 | Dhrs9 | dehydrogenase/reductase (SD... | NM_175512.2 | 85.7% | 85.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1023
- ORF length:
- 957
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct cttttgggtg ctaggcctcc taatcctctg tggttttctg tggactcgta 121 aaggaaaact aaagattgaa gacatcactg ataagtacat ttttatcact ggatgtgact 181 cgggctttgg aaacttggca gccagaactt ttgataaaaa gggatttcat gtaatcgctg 241 cctgtctgac tgaatcagga tcaacagctt taaaggcaga aacctcagag agacttcgta 301 ctgtgcttct ggatgtgacc gacccagaga atgtcaagag gactgcccag tgggtgaaga 361 accaagttgg ggagaaaggt ctctggggtc tgatcaataa tgctggtgtt cccggcgtgc 421 tggctcccac tgactggctg acactagagg actacagaga acctattgaa gtgaacctgt 481 ttggactcat cagtgtgaca ctaaatatgc ttcctttggt caagaaagct caagggagag 541 ttattaatgt ctccagtgtt ggaggtcgcc ttgcaatcgt tggaggggGC TATACTCCAT 601 CCAAATATGC AGTGGAAGGT TTCAATGACA GCTTAAGACG GGACATGAAA GCTTTTGGTG 661 TGCACGTCTC ATGCATTGAA CCAGGATTGT TCAAAACAAA CTTGGCAGAT CCAGTAAAGG 721 TAATTGAAAA AAAACTCGCC ATTTGGGAGC AGCTGTCTCC AGACATCAAA CAACAATATG 781 GAGAAGGTTA CATTGAAAAA AGTCTAGACA AACTGAAAGG CAATAAATCC TATGTGAACA 841 TGGACCTCTC TCCGGTGGTA GAGTGCATGG ACCACGCTCT AACAAGTCTC TTCCCTAAGA 901 CTCATTATGC CGCTGGAAAA GATGCCAAAA TTTTCTGGAT ACCTCTGTCT CACATGCCAG 961 CAGCTTTGCA AGACTTTTTA TTGTTGAAAC AGAAAGCAGA GCTGGCTAAT CCCAAGGCAG 1021 TGTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1081 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1141 GGCTTTATAT ATCTTGTGGA AAGGACGATT TTATATCTCA CATAGTTCCG ATACGCGTTA 1201 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt