Construct: ORF TRCN0000473037
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014111.1_s317c1
- Derived from:
- ccsbBroadEn_09380
- DNA Barcode:
- CGAATACGAGCAAAGGAGGGCTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HOGA1 (112817)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473037
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 112817 | HOGA1 | 4-hydroxy-2-oxoglutarate al... | NM_138413.4 | 99.8% | 100% | 912C>A |
| 2 | human | 112817 | HOGA1 | 4-hydroxy-2-oxoglutarate al... | NM_001134670.1 | 50% | 50.1% | 210_211ins489;423C>A |
| 3 | mouse | 67432 | Hoga1 | 4-hydroxy-2-oxoglutarate al... | NM_026152.1 | 85.4% | 87.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1047
- ORF length:
- 981
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gggtccccaa gtctggtctt ctgtgaggca ggggctaagc aggagcttgt 121 ccaggaatgt gggggtctgg gcctcagggg aggggaagaa ggtggacatt gcgggtatct 181 acccccctgt gaccaccccc ttcactgcca ctgcagaggt ggactatggg aaactggagg 241 agaatctgca caaactgggc accttcccct tccgaggctt cgtggtccag ggctccaatg 301 gcgagtttcc tttcctgacc agcagtgagc gcctcgaggt ggtgagccgt gtgcgccagg 361 ccatgcccaa gaacaggctc ctgctagctg gctccggatg cgagtccact caagccacag 421 tggagatgac cgtcagcatg gcccaggtcg gggctgacgc ggccatggtg gtgacccctt 481 gctactatcg tggccgcatg agcagtgcgg ccctcattca ccactacacc aaggttgctg 541 atctctctcc aatccctgtg gtgctgtaca gtgtcccagc caaCACAGGG CTGGACCTGC 601 CTGTGGATGC AGTGGTCACG CTTTCCCAGC ACCCGAATAT TGTGGGCATG AAGGACAGCG 661 GTGGTGATGT GACCAGGATT GGGCTGATTG TTCACAAGAC CAGGAAGCAG GATTTTCAGG 721 TGTTGGCTGG ATCGGCTGGC TTTCTGATGG CCAGCTATGC CTTGGGAGCT GTGGGGGGCG 781 TCTGCGCCCT GGCCAATGTC CTGGGGGCTC AGGTGTGCCA GCTGGAGCGA CTGTGCTGCA 841 CGGGGCAATG GGAAGATGCC CAGAAACTGC AGCACCGCCT CATTGAGCCA AACGCTGCGG 901 TGACCCGGCG CTTTGGGATC CCAGGGCTGA AGAAAATCAT GGACTGGTTT GGCTACTATG 961 GAGGCCCCTG CCGCGCACCC TTGCAGGAGC TGAGCCCCGC TGAGGAGGAG GCACTGCGCA 1021 TGGATTTCAC CAGCAACGGC TGGCTCTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 1081 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1141 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACGAATACG 1201 AGCAAAGGAG GGCTTCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1261 aagatt