Construct: ORF TRCN0000473039
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013594.1_s317c1
- Derived from:
- ccsbBroadEn_16122
- DNA Barcode:
- CAAGTTCAAGTTGATTCGAACCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADAM32 (203102)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473039
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 203102 | ADAM32 | ADAM metallopeptidase domai... | NM_145004.7 | 99.8% | 99.6% | 293A>G;478A>G;1400C>G |
| 2 | human | 203102 | ADAM32 | ADAM metallopeptidase domai... | XM_006716298.3 | 98.2% | 97.9% | (many diffs) |
| 3 | human | 203102 | ADAM32 | ADAM metallopeptidase domai... | XM_011544433.2 | 98.2% | 97.9% | (many diffs) |
| 4 | human | 203102 | ADAM32 | ADAM metallopeptidase domai... | XM_017013183.2 | 96.9% | 96.6% | (many diffs) |
| 5 | human | 203102 | ADAM32 | ADAM metallopeptidase domai... | XM_017013184.2 | 94.9% | 94.6% | (many diffs) |
| 6 | human | 203102 | ADAM32 | ADAM metallopeptidase domai... | XM_017013185.2 | 94% | 93.7% | (many diffs) |
| 7 | human | 203102 | ADAM32 | ADAM metallopeptidase domai... | XM_024447092.1 | 93.2% | 92.6% | (many diffs) |
| 8 | human | 203102 | ADAM32 | ADAM metallopeptidase domai... | XM_011544434.2 | 90.5% | 90.2% | (many diffs) |
| 9 | human | 203102 | ADAM32 | ADAM metallopeptidase domai... | XM_006716299.2 | 86.4% | 86.1% | (many diffs) |
| 10 | human | 203102 | ADAM32 | ADAM metallopeptidase domai... | XM_017013186.2 | 83.3% | 83.1% | (many diffs) |
| 11 | human | 203102 | ADAM32 | ADAM metallopeptidase domai... | XM_024447093.1 | 79.7% | 79.1% | (many diffs) |
| 12 | human | 203102 | ADAM32 | ADAM metallopeptidase domai... | NM_001313994.1 | 79.6% | 78.9% | (many diffs) |
| 13 | human | 203102 | ADAM32 | ADAM metallopeptidase domai... | XM_017013187.1 | 64.6% | 64.5% | 0_1ins831;3_5delGAG;572C>G |
| 14 | human | 203102 | ADAM32 | ADAM metallopeptidase domai... | XM_017013188.1 | 59.1% | 59% | 0_1ins963;437C>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2427
- ORF length:
- 2361
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt ccgcctctgg ttgctgctgg ccgggctctg cggcctcctg gcgtcaagac 121 ccggttttca aaattcactt ctacagatcg taattccaga gaaaatccaa acaaatacaa 181 atgacagttc agaaatagaa tatgaacaaa tatcctatat tattccaata gatgagaaac 241 tgtacactgt gcaccttaaa caaagatatt ttttagcaga taattttatg atctatttgt 301 acaatcaagg atctatgaat acttattctt cagatattca gactcaatgc tactatcgag 361 gaaatattga aggatatcca gattccatgg tcacactcag cacgtgctct ggactaagag 421 gaatactgca atttgaaaat gtttcttatg gaattgagcc tctggaatct gcagttgaat 481 ttcagcatgt tctttacaaa ttaaagaatg aagacaatga tattgcaatt tttattgaca 541 gaggcctgaa agaacaacca atggatgaca acatttttat aagtgaaaaa tcagaaccag 601 ctgttccaga tttatttcct ctttatctag aaatgcatat tgtggtggac aaaactttgt 661 atgattactg gggctctgat agcatgatag taacaaataa agtcatcgaa attgttggcc 721 ttgcaaattc aatgttcacc caatttaaag ttactattgt gctgtcatca ttggagttat 781 ggtcagatga aaataagatt tctacagttg gtgaggcaga tgaattattg caaaaatttt 841 tagaatggaa acaatcttat cttaacctaa ggcctcatga tattgcatat ctactaattt 901 atatggatta tcctcgttat ttgggagcag tgtttcctgg aacaatgtgt attactcgtt 961 attctgcagg agttgcattg taccccaagg agataactct ggaggcattt gcagttattg 1021 tcacccagat gctggcactc agtctgggaa tatcatatga cgacccaaag aaatgtcaat 1081 gttcagaatc cacctgtata atgaatccag aagttgtgca atccaatggt gtgaagactt 1141 ttagcagttg cagtttgagg agctttcaaa atttcatttc aaatgtgggt gtcaaatgtc 1201 ttcagaataa gccacaaatg caaaaaaaat ctccgaaacc agtctgtggc aatggcagat 1261 tggagggaaa tgaaatctgt gattgtggta ctgaggctca atgtggacct gcaagctgtt 1321 gtgattttcg aacttgtgta ctgaaagacg gagcaaaatg ttataaagga ctgtgctgca 1381 aagactgtca aattttacaa tcaggcgttg aatgtaggcc gaaagcacat cctgaatgtg 1441 acatcgctga aaattgtaat ggaagctcac cagaatgtgg tcctgacata actttaatca 1501 atggactttc atgcaaaaat aataagttta tttgttatga cggagactgc catgatctcg 1561 atgcacgttg tgagagtgta tttggaaaag gttcaagaaa tgctccattt gcctgctatg 1621 aagaaataca atctcaatca gacagatttg ggaactgtgg tagggataga aataacaaat 1681 atgtgttctg tggatggagg aatcttatat gtggaagatt agtttgtacc taccctactc 1741 gaaagccttt ccatcaagaa aatggtgatg tgatttatgc tttcgtacga gattctgtat 1801 gcataactgt agactacaaa ttgcctcgaa cagttccaga tccactggct gtcaaaaatg 1861 gctctcagtg tgatattggg agggtttgtg taaatcgtga atgtgtagaa tcaaggataa 1921 ttaaggcttc agcacatgtt tgttcacaac agtgttctgg acatggagtg tgtgattcca 1981 gaaacaagtg ccattgttcg ccaggctata agcctccaaa ctgccaaata cgttcCAAAG 2041 GATTTTCCAT ATTTCCTGAG GAAGATATGG GTTCAATCAT GGAAAGAGCA TCTGGGAAGA 2101 CTGAAAACAC CTGGCTTCTA GGTTTCCTCA TTGCTCTTCC TATTCTCATT GTAACAACCG 2161 CAATAGTTTT GGCAAGGAAA CAGTTGAAAA AGTGGTTCGC CAAGGAAGAG GAATTCCCAA 2221 GTAGCGAATC TAAATCGGAA GGTAGCACAC AGACATATGC CAGCCAATCC AGCTCAGAAG 2281 GCAGCACTCA GACATATGCC AGCCAAACCA GATCAGAAAG CAGCAGTCAA GCTGATACTA 2341 GCAAATCCAA ATCAGAAGAT AGTGCTGAAG CATATACTAG CAGATCCAAA TCACAGGACA 2401 GTACCCAAAC ACAAAGCAGT AGTAACTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 2461 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 2521 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACAAGTTCA 2581 AGTTGATTCG AACCAAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 2641 aagatt