Construct: ORF TRCN0000473043
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002248.1_s317c1
- Derived from:
- ccsbBroadEn_06349
- DNA Barcode:
- CGGACTTTACTCCAGCCACCACTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HAGH (3029)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473043
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3029 | HAGH | hydroxyacylglutathione hydr... | NM_001040427.2 | 99.8% | 100% | 279C>T |
| 2 | human | 3029 | HAGH | hydroxyacylglutathione hydr... | NM_001363914.1 | 90.7% | 86.5% | (many diffs) |
| 3 | human | 3029 | HAGH | hydroxyacylglutathione hydr... | NM_005326.6 | 84.3% | 84.4% | 1_144del;423C>T |
| 4 | human | 3029 | HAGH | hydroxyacylglutathione hydr... | NM_001363912.1 | 76.9% | 73.4% | (many diffs) |
| 5 | human | 3029 | HAGH | hydroxyacylglutathione hydr... | XM_024450249.1 | 72.1% | 42.9% | 279C>T;285_286ins109;564_565ins107 |
| 6 | human | 3029 | HAGH | hydroxyacylglutathione hydr... | NM_001286249.2 | 60.9% | 36.6% | (many diffs) |
| 7 | human | 3029 | HAGH | hydroxyacylglutathione hydr... | XM_011522470.3 | 60.9% | 36.6% | (many diffs) |
| 8 | mouse | 14651 | Hagh | hydroxyacyl glutathione hyd... | NM_001159626.1 | 85.8% | 91.1% | (many diffs) |
| 9 | mouse | 14651 | Hagh | hydroxyacyl glutathione hyd... | XM_006523660.2 | 85.8% | 91.1% | (many diffs) |
| 10 | mouse | 14651 | Hagh | hydroxyacyl glutathione hyd... | XM_006523661.1 | 85.8% | 91.1% | (many diffs) |
| 11 | mouse | 14651 | Hagh | hydroxyacyl glutathione hyd... | NM_024284.2 | 72.2% | 76.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 846
- ORF length:
- 780
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 tgggcatgaa ggtagaggtg ctgcctgccc tgaccgacaa ctacatgtac ctggtcattg 121 atgatgagac caaggaggct gccattgtgg atccggtgca gccccagaag gtcgtggacg 181 cggcgagaaa gcacggggtg aaactgacca cagtgctcac cacccaccac cactgggacc 241 atgctggcgg gaatgagaaa ctggtcaagc tggagtcggg actgaaggtg tacgggggtg 301 acgaccgtat cggggccctG ACTCACAAGA TCACTCACCT GTCTACACTG CAGGTGGGGT 361 CTCTGAACGT CAAGTGCCTG GCGACCCCGT GCCACACTTC AGGACACATT TGTTACTTCG 421 TGAGCAAGCC CGGAGGCTCG GAGCCCCCTG CCGTGTTCAC AGGTGACACC TTGTTTGTGG 481 CTGGCTGCGG GAAGTTCTAT GAAGGGACTG CGGATGAGAT GTGTAAAGCT CTGCTGGAGG 541 TCTTGGGCCG GCTCCCCCCG GACACAAGAG TCTACTGTGG CCACGAGTAC ACCATCAACA 601 ACCTCAAGTT TGCACGCCAC GTGGAGCCCG GCAATGCCGC CATCCGGGAG AAGCTGGCCT 661 GGGCCAAGGA GAAGTACAGC ATCGGGGAGC CCACAGTGCC ATCCACCCTG GCAGAGGAGT 721 TTACCTACAA CCCCTTCATG AGAGTGAGGG AGAAGACGGT GCAGCAGCAC GCAGGTGAGA 781 CGGACCCGGT GACCACCATG CGGGCCGTGC GCAGGGAGAA GGACCAGTTC AAGATGCCCC 841 GGGACTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 901 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 961 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACGGACTTTA CTCCAGCCAC CACTGACGCG 1021 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt