Transcript: Mouse XM_006523661.1

PREDICTED: Mus musculus hydroxyacyl glutathione hydrolase (Hagh), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Hagh (14651)
Length:
1215
CDS:
275..1057

Additional Resources:

NCBI RefSeq record:
XM_006523661.1
NBCI Gene record:
Hagh (14651)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006523661.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000101752 TGGTCATGAATACACCGTAAA pLKO.1 787 CDS 100% 10.800 15.120 N Hagh n/a
2 TRCN0000101754 GTGGTCATGAATACACCGTAA pLKO.1 786 CDS 100% 4.050 5.670 N Hagh n/a
3 TRCN0000312116 GTGGTCATGAATACACCGTAA pLKO_005 786 CDS 100% 4.050 5.670 N Hagh n/a
4 TRCN0000313056 GACTGTGCAGCAAGACATAAT pLKO_005 256 5UTR 100% 13.200 10.560 N Hagh n/a
5 TRCN0000313057 TGCAACCACAGAAGGTTATAG pLKO_005 366 CDS 100% 13.200 9.240 N Hagh n/a
6 TRCN0000101751 GCTGCCATTCAAGAGAAACTA pLKO.1 845 CDS 100% 5.625 3.938 N Hagh n/a
7 TRCN0000312062 GCTGCCATTCAAGAGAAACTA pLKO_005 845 CDS 100% 5.625 3.938 N Hagh n/a
8 TRCN0000101753 CTGTGTTCACAGGTGACACAT pLKO.1 660 CDS 100% 0.495 0.347 N Hagh n/a
9 TRCN0000101750 CGCTGGGACAGGGACTTCTAA pLKO.1 1068 3UTR 100% 1.875 1.125 N Hagh n/a
10 TRCN0000312063 CGCTGGGACAGGGACTTCTAA pLKO_005 1068 3UTR 100% 1.875 1.125 N Hagh n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006523661.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00724 pDONR223 100% 86% 91.1% None (many diffs) n/a
2 TRCN0000479250 GGTTCCCATTCTACTTTAGTTATT pLX_317 45.7% 86% 91.1% V5 (many diffs) n/a
3 ccsbBroadEn_06349 pDONR223 100% 85.8% 91.1% None (many diffs) n/a
4 ccsbBroad304_06349 pLX_304 0% 85.8% 91.1% V5 (many diffs) n/a
5 TRCN0000473043 CGGACTTTACTCCAGCCACCACTG pLX_317 67.1% 85.8% 91.1% V5 (many diffs) n/a
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