Construct: ORF TRCN0000473054
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008997.1_s317c1
- Derived from:
- ccsbBroadEn_07320
- DNA Barcode:
- TTTGGGCAGAGCAACTTGACTTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SQSTM1 (8878)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473054
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8878 | SQSTM1 | sequestosome 1 | NM_003900.5 | 99.7% | 99.7% | 876C>T;905G>A;936G>A |
| 2 | human | 8878 | SQSTM1 | sequestosome 1 | NM_001142298.2 | 80.6% | 80.6% | (many diffs) |
| 3 | human | 8878 | SQSTM1 | sequestosome 1 | NM_001142299.2 | 80.6% | 80.6% | (many diffs) |
| 4 | mouse | 18412 | Sqstm1 | sequestosome 1 | NM_011018.3 | 86.4% | 90.4% | (many diffs) |
| 5 | mouse | 18412 | Sqstm1 | sequestosome 1 | NM_001290769.1 | 78.4% | 81.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1386
- ORF length:
- 1320
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gtcgctcacc gtgaaggcct accttctggg caaggaggac gcggcgcgcg 121 agattcgccg cttcagcttc tgctgcagcc ccgagcctga ggcggaagcc gaggctgcgg 181 cgggtccggg accctgcgag cggctgctga gccgggtggc cgccctgttc cccgcgctgc 241 ggcctggcgg cttccaggcg cactaccgcg atgaggacgg ggacttggtt gccttttcca 301 gtgacgagga attgacaatg gccatgtcct acgtgaagga tgacatcttc cgaatctaca 361 ttaaagagaa aaaagagtgc cggcgggacc accgcccacc gtgtgctcag gaggcgcccc 421 gcaacatggt gcaccccaat gtgatctgcg atggctgcaa tgggcctgtg gtaggaaccc 481 gctacaagtg cagcgtctgc ccagactacg acttgtgtag cgtctgcgag ggaaagggct 541 tgcaccgggg gcacaccaag ctcgcattcc ccagcccctt cgggcacctg tctgagggct 601 tctcgcacag ccgctggctc cggaaggtga aacacggaca cttcgggtgg ccaggatggg 661 aaatgggtcc accaggaaac tggagcccac gtcctcctcg tgcaggggag gcccgccctg 721 gccccacggc agaatcagct tctggtccat cggaggatcc gagtgtgaat ttcctgaaga 781 acgttgggga gagtgtggca gctgccctta gccctctggg cattgaagtt gatatcgatg 841 tggagcaCGG AGGGAAAAGA AGCCGCCTGA CCCCCGTCTC TCCAGAGAGT TCCAGCACAG 901 AGGAGAAGAG CAGCTCACAG CCAAGCAGCT GCTGCTCTGA TCCCAGCAAG CCGGGTGGGA 961 ATGTTGAGGA CGCCACGCAG TCTCTGGCGG AGCAGATGAG AAAGATCGCC TTGGAGTCCG 1021 AGGGGCGCCC TGAGGAACAG ATGGAGTCGG ATAACTGTTC AGGAGGAGAT GATGACTGGA 1081 CCCATCTGTC TTCAAAAGAA GTGGACCCGT CTACAGGTGA ACTCCAGTCC CTACAGATGC 1141 CAGAATCCGA AGGGCCAAGC TCTCTGGACC CCTCCCAGGA GGGACCCACA GGGCTGAAGG 1201 AAGCTGCCTT GTACCCACAT CTCCCGCCAG AGGCTGACCC GCGGCTGATT GAGTCCCTCT 1261 CCCAGATGCT GTCCATGGGC TTCTCTGATG AAGGCGGCTG GCTCACCAGG CTCCTGCAGA 1321 CCAAGAACTA TGACATCGGA GCGGCTCTGG ACACCATCCA GTATTCAAAG CATCCCCCGC 1381 CGTTGTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1441 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1501 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATTTGGGCAG AGCAACTTGA CTTTTACGCG 1561 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt