Construct: ORF TRCN0000473066
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018936.3_s317c1
- Derived from:
- ccsbBroadEn_14794
- DNA Barcode:
- AGCCCACTGGCGACGGCTTTAACC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PRKD1 (5587)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473066
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5587 | PRKD1 | protein kinase D1 | NM_002742.3 | 99.8% | 23.5% | (many diffs) |
| 2 | human | 5587 | PRKD1 | protein kinase D1 | NM_001330069.2 | 98.9% | 23.3% | (many diffs) |
| 3 | human | 5587 | PRKD1 | protein kinase D1 | NM_001348390.1 | 88.5% | 12.9% | (many diffs) |
| 4 | human | 5587 | PRKD1 | protein kinase D1 | XM_011536965.2 | 88.5% | 12.9% | (many diffs) |
| 5 | human | 5587 | PRKD1 | protein kinase D1 | XM_017021462.1 | 81.7% | 5.4% | (many diffs) |
| 6 | human | 5587 | PRKD1 | protein kinase D1 | XR_943493.2 | 79% | (many diffs) | |
| 7 | mouse | 18760 | Prkd1 | protein kinase D1 | XM_006515587.1 | 87.6% | 21.8% | (many diffs) |
| 8 | mouse | 18760 | Prkd1 | protein kinase D1 | NM_008858.3 | 86.8% | 21.6% | (many diffs) |
| 9 | mouse | 18760 | Prkd1 | protein kinase D1 | XM_006515586.1 | 86.7% | 21.6% | (many diffs) |
| 10 | mouse | 18760 | Prkd1 | protein kinase D1 | XM_006515590.3 | 71.4% | 5.1% | (many diffs) |
| 11 | mouse | 18760 | Prkd1 | protein kinase D1 | XM_011244017.1 | 71.4% | 5.1% | (many diffs) |
| 12 | mouse | 18760 | Prkd1 | protein kinase D1 | XM_006515591.1 | 70.5% | 26.5% | (many diffs) |
| 13 | mouse | 18760 | Prkd1 | protein kinase D1 | XR_001780432.1 | 54.5% | (many diffs) | |
| 14 | mouse | 18760 | Prkd1 | protein kinase D1 | XR_001780431.1 | 52.2% | (many diffs) | |
| 15 | mouse | 18760 | Prkd1 | protein kinase D1 | XR_001780430.1 | 52.2% | (many diffs) | |
| 16 | mouse | 18760 | Prkd1 | protein kinase D1 | XR_001780427.1 | 38% | (many diffs) | |
| 17 | mouse | 18760 | Prkd1 | protein kinase D1 | XR_001780429.1 | 37.3% | (many diffs) | |
| 18 | mouse | 18760 | Prkd1 | protein kinase D1 | XR_001780428.1 | 37.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 729
- ORF length:
- 660
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagcgcccct ccggtcctgc ggccgcccag tccgctgctg cccgtggcgg 121 cggcagctgc cgcagcggcc gccgcactgg tcccagggtc cgggcccggg cccgcgccgt 181 tcttggctcc tgtcgcggcc ccggtcgggg gcatctcgtt ccatctgcag atcggcctga 241 gccgtgagcc ggtgctgctg ctgcaggact cgtccgggga ctacagcctg gcgcacgtcc 301 gcgagatggc ttgctccatt gtcgaccaga agttccctga atgtggtttc tacggaatgt 361 atgataagat cctgcttttt cgccatgacc ctacctctga aaacatcctt cagctggtga 421 aagcggccag tgatatccag gaaggcgatc ttattgaagt ggtcttgtca gcttccgcca 481 cctttgaaga ctttcagatt cgtccccacg ctctctttgt tcattcatac agagctccag 541 ctttctgtga tcactgtgga gaaatgctgt gggggctggt acgtcaaggt cttaaatgtg 601 aagggtgtgg tctgaattac cataagagat gtgcatttaa aatacccaac aattgcagcg 661 gtgtgaggcg gagaacgctc tcaaacgttt ccctcactgg ggtcagcacc atccggcaca 721 tcatctgctg aactctctac aagtgcccct gatgagcccc ttctgcaaaa atcaccatca 781 gagtcgttta ttggtcgaga gaagaggtca aattctcaat catacattgg acgaccaatt 841 caccttgaca agattttgat gtctaaagtt aaagtgccgc acacatttgt catccactcc 901 tacacccggc ccacagtgtg ccagtactgc aagaagcttc tgaaggggct tttcaggcag 961 ggcttgcagt gcaaagattg cagattcaac tgccataaac gttgtgcacc gaaagtacca 1021 aacaactgcc ttggcgaagt gaccattaat ggagatttgc ttagccctgg ggcagagtct 1081 gatgtggtca tggaagaagg gagtgatgac aatgatagtg aaaggaacag tgggctcatg 1141 gatgatatgg aagaagcaat ggtccaagat gcagagatgg caatggcaga gtgccagaac 1201 gacagtggcg agatgcaaga tccagaccca gaccacgagg acgccaacag aaccatcagt 1261 ccatcaacaa gcaacaatat cccactcatg agggtagtgc agtctgtcaa acacacgaag 1321 aggaaaagca gcacagtcat gaaagaagga tggatggtcc actacaccag caaggacacg 1381 ctgcggaaac ggcactattg gagattggat agcaaatgta ttaccctctt tcagaatgac 1441 acaggaagca ggtactacaa ggaaattcct ttatctgaaa ttttgtctct ggaaccagta 1501 aaaacttcag ctttaattcc taatggggcc aatcctcatt gtttcgaaat cactacggca 1561 aatgtagtgt attatgtggg agaaaatgtg gtcaatcctt ccagcccatc accaaataac 1621 agtgttctca ccagtggcgt tggtgcagat gtggccagga tgtgggagat agccatccag 1681 catgccctta tgcccgtcat tcccaagggc tcctccgtgg gtacaggaac caacttgcac 1741 agagatatct ctgtgagtat ttcagtatca aattgccaga ttcaagaaaa tgtggacatc 1801 agcacagtat atcagatttt tcctgatgaa gtactgggtt ctggacagtt tggaattgtt 1861 tatggaggaa aacatcgtaa aacaggaaga gatgtagcta ttaaaatcat tgacaaatta 1921 cgatttccaa caaaacaaga aagccagctt cgtaatgagg ttgcaattct acagaacctt 1981 catcaccctg gtgttgtaaa tttggagtgt atgtttgaga cgcctgaaag agtgtttgtt 2041 gttatggaaa aactccatgg agacatgctg gaaatgatcc ttgtcaagtg aaaagggcag 2101 gttgccagag cacataacga agtttttaat tactcagata ctcgtggctt tgcggcactt 2161 tcattttaaa aatatcgttc actgtgacct caaaccagaa aatgtgttgc tagcctcagc 2221 tgatcctttt cctcaggtga aactttgtga ttttggtttt gcccggatca tcggagagaa 2281 gtctttccgg aggtcagtgg tgggtacccc cgcttacctg gctcctgagg tcctaaggaa 2341 caagggctac aatcgctctc tagacatgtg gtctgttggg gtcatcatct atgtaagccT 2401 AAGCGGCACA TTCCCATTTA ATGAAGATGA AGACATACAC GACCAAATTC AGAATGCAGC 2461 TTTCATGTAT CCACCAAATC CCTGGAAGGA AATATCTCAT GAAGCCATTG ATCTTATCAA 2521 CAATTTGCTG CAAGTAAAAA TGAGAAAGCG CTACAGTGTG GATAAGACCT TGAGCCACCC 2581 TTGGCTACAG GACTATCAGA CCTGGTTAGA TTTGCGAGAG CTGGAATGCA AAATCGGGGA 2641 GCGCTACATC ACCCATGAAA GTGATGACCT GAGGTGGGAG AAGTATGCAG GCGAGCAGGG 2701 GCTGCAGTAC CCCACACACC TGATCAATCC AAGTGCTAGC CACAGTGACA CTCCTGAGAC 2761 TGAAGAAACA GAAATGAAAG CCCTCGGTGA GCGTGTCAGC ATCCTCTTGC CAACTTTCTT 2821 GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC 2881 GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG 2941 TGGAAAGGAC GAAGCCCACT GGCGACGGCT TTAACCACGC GTTAAGTCga caatcaacct 3001 ctggattaca aaatttgtga aagatt