Construct: ORF TRCN0000473075
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005096.1_s317c1
- Derived from:
- ccsbBroadEn_04706
- DNA Barcode:
- ATGAGACTATCCGAGAGTATTGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RBP7 (116362)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473075
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 116362 | RBP7 | retinol binding protein 7 | NM_052960.3 | 100% | 100% | |
| 2 | human | 116362 | RBP7 | retinol binding protein 7 | NR_138250.2 | 47.8% | 1_31del;103_317del;649_840del | |
| 3 | mouse | 63954 | Rbp7 | retinol binding protein 7, ... | NM_022020.2 | 87% | 89.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 468
- ORF length:
- 402
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc cgccgacctc agcggtactt ggaccctgct cagcagcGAC AACTTCGAGG 121 GCTACATGCT GGCCCTAGGT ATTGACTTTG CCACTCGTAA AATAGCCAAG TTGCTGAAGC 181 CACAGAAAGT GATTGAGCAG AATGGGGATT CTTTTACCAT CCACACGAAC AGCAGCCTAA 241 GGAACTACTT TGTGAAATTT AAAGTTGGAG AAGAATTTGA TGAAGATAAC AGAGGCCTGG 301 ACAACAGAAA ATGCAAGAGT TTGGTTATCT GGGACAATGA CAGGCTCACC TGTATCCAGA 361 AGGGAGAAAA GAAGAACAGA GGCTGGACCC ATTGGATCGA AGGAGACAAA CTCCACCTGG 421 AAATGTTCTG TGAAGGTCAA GTGTGCAAAC AGACATTCCA GAGAGCCTGC CCAACTTTCT 481 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 541 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 601 GTGGAAAGGA CGAATGAGAC TATCCGAGAG TATTGTAACG CGTTAAGTCg acaatcaacc 661 tctggattac aaaatttgtg aaagatt