Construct: ORF TRCN0000473080
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013961.1_s317c1
- Derived from:
- ccsbBroadEn_02097
- DNA Barcode:
- ACCTCATCTAAACCTAAACACCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LPAR2 (9170)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473080
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9170 | LPAR2 | lysophosphatidic acid recep... | NM_004720.6 | 100% | 100% | |
| 2 | human | 9170 | LPAR2 | lysophosphatidic acid recep... | XM_017027468.2 | 99.9% | 100% | 864C>A |
| 3 | human | 9170 | LPAR2 | lysophosphatidic acid recep... | XM_017027469.1 | 99.9% | 100% | 864C>A |
| 4 | human | 9170 | LPAR2 | lysophosphatidic acid recep... | XM_024451774.1 | 99.9% | 100% | 864C>A |
| 5 | mouse | 53978 | Lpar2 | lysophosphatidic acid recep... | NM_020028.3 | 85.8% | 91.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1119
- ORF length:
- 1053
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt catcatgggc cagtgctact acaacgagac catcggcttc ttctataaca 121 acagtggcaa agagctcagc tcccactggc ggcccaagga tgtggtcgtg gtggcactgg 181 ggctgaccgt cagcgtgctg gtgctgctga ccaatctgct ggtcatagca gccatcgcct 241 ccaaccgccg cttccaccag cccatctact acctgctcgg caatctggcc gcggctgacc 301 tcttcgcggg cgtggcctac ctcttcctca tgttccacac tggtccccgc acagcccgac 361 tttcacttga gggctggttc ctgcggcagg gcttgctgga cacaagcctc actgcgtcgg 421 tggccacact gctggccatc gccgtggagc ggcaccgcag tgtgatggcc gtgcagctgc 481 acagccgcct gccccgtggc cgcgtggtca tgctcattgt gggcgtgtgg gtggctgccc 541 tgggcctggg gctgctgcct gcccactcct ggcactgcct ctgtgccctg gaccgctgct 601 cacgcatggc acccctgctc agccgctcct atttggccgt ctgggctctg tcgagcctgc 661 ttgtcttcct gctcatggtg gctgtgtaca cccgcatttt cttctacgtg cggcggcgag 721 tgcagcgcat ggcagagcat gtcagctgcc acccccgcta ccgagagacc acgctcagcc 781 tggtcaagac tgttgtcatc atcctggggg cgttcgtggt ctgctggaca ccaggccagg 841 tggtactgct cctggatggt ttaggctgtg agtccTGCAA TGTCCTGGCT GTAGAAAAGT 901 ACTTCCTACT GTTGGCCGAG GCCAACTCAC TGGTCAATGC TGCTGTGTAC TCTTGCCGAG 961 ATGCTGAGAT GCGCCGCACC TTCCGCCGCC TTCTCTGCTG CGCGTGCCTC CGCCAGTCCA 1021 CCCGCGAGTC TGTCCACTAT ACATCCTCTG CCCAGGGAGG TGCCAGCACT CGCATCATGC 1081 TTCCCGAGAA CGGCCACCCA CTGATGGACT CCACCCTTTA CCCAACTTTC TTGTACAAAG 1141 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1201 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1261 ACGAACCTCA TCTAAACCTA AACACCCGAC GCGTTAAGTC gacaatcaac ctctggatta 1321 caaaatttgt gaaagatt