Construct: ORF TRCN0000473095
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007796.1_s317c1
- Derived from:
- ccsbBroadEn_02061
- DNA Barcode:
- CATCTCTTCATCAAACAACCATTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATP6V0E1 (8992)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473095
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8992 | ATP6V0E1 | ATPase H+ transporting V0 s... | NM_003945.4 | 100% | 100% | |
| 2 | mouse | 11974 | Atp6v0e | ATPase, H+ transporting, ly... | NM_025272.2 | 90.5% | 98.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 223
- ORF end:
- 466
- ORF length:
- 243
- Sequence:
-
1 AGGGGTTTTA TGCGATGGAG TTTCCCCACA CTGAGTGGGT GGAGACTGAA GTTAGGCCAG 61 CTTGGCACTT GATGTAATTC TCCTTGGAAT TTGCCCTTTT TGAGTTTGGA TCTTGGTTCA 121 TTCTCAAGCC TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT 181 AGCATCGATT GATCAACAAG TTTGTACAAA AAAGTTGGCA CCATGGCGTA TCACGGCCTC 241 ACTGTGCCTC TCATTGTGAT GAGCGTGTTC TGGGGCTTCG TCGGCTTCTT GGTGCCTTGG 301 TTCATCCCTA AGGGTCCTAA CCGGGGAGTT ATCATTACCA TGTTGGTGAC CTGTTCAGTT 361 TGCTGCTATC TCTTTTGGCT GATTGCAATT CTGGCCCAAC TCAACCCTCT CTTTGGACCG 421 CAATTGAAAA ATGAAACCAT CTGGTATCTG AAGTATCATT GGCCTTTGCC AACTTACTTG 481 TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG 541 TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT 601 GGAAAGGACG ACATCTCTTC ATCAAACAAC CATTCACGCG TTAAGTCgac aatcaacctc 661 tggattacaa aatttgtgaa agatt