Construct: ORF TRCN0000473098
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016288.1_s317c1
- Derived from:
- ccsbBroadEn_12343
- DNA Barcode:
- CATCTACGTTTCGCAGTCTTGCAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CLK4 (57396)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473098
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57396 | CLK4 | CDC like kinase 4 | NM_020666.3 | 11.1% | 11% | 162_1443delinsG |
| 2 | mouse | 12750 | Clk4 | CDC like kinase 4 | NM_007714.6 | 9.9% | 10.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 231
- ORF end:
- 393
- ORF length:
- 162
- Sequence:
-
1 AGGTTGGGGG GAGGGGTTTT ATGCGATGGA GTTTCCCCAC ACTGAGTGGG TGGAGACTGA 61 AGTTAGGCCA GCTTGGCACT TGATGTAATT CTCCTTGGAA TTTGCCCTTT TTGAGTTTGG 121 ATCTTGGTTC ATTCTCAAGC CTCAGACAGT GGTTCAAAGT TTTTTTCTTC CATTTCAGGT 181 GTCGTGAGGC TAGCATCGAT TGATCAACAA GTTTGTACAA AAAAGTTGGC ATGCGGCATT 241 CCAAAAGAAC TCACTGTCCT GATTGGGATA GCAGAGAAAG CTGGGGACAT GAAAGCTATC 301 GTGGAAGTCA CAAGCGGAAG AGGAGATCTC ATAGTAGCAC ACAAGAGAAC AGGCATTGTA 361 AACCACATCA CCAGTTTAAA GAATCTGATT GGTACCCAAC TTTCTTGTAC AAAGTGGTTG 421 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 481 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACA 541 TCTACGTTTC GCAGTCTTGC AGACGCGTTA AGTCgacaat caacctctgg attacaaaat 601 ttgtgaaaga tt