Construct: ORF TRCN0000473101
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012247.1_s317c1
- Derived from:
- ccsbBroadEn_10071
- DNA Barcode:
- CGGACAAGGGTAATGTCTGTTTCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C5orf34 (375444)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473101
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 375444 | C5orf34 | chromosome 5 open reading f... | NM_198566.4 | 99.9% | 100% | 333A>G |
| 2 | human | 375444 | C5orf34 | chromosome 5 open reading f... | XM_011514038.3 | 88.3% | 87.1% | (many diffs) |
| 3 | human | 375444 | C5orf34 | chromosome 5 open reading f... | XM_017009445.1 | 82.1% | 82.1% | 0_1ins342 |
| 4 | human | 375444 | C5orf34 | chromosome 5 open reading f... | XM_017009446.1 | 82.1% | 82.1% | 0_1ins342 |
| 5 | human | 375444 | C5orf34 | chromosome 5 open reading f... | XR_001742063.2 | 71.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1980
- ORF length:
- 1914
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agctgaactg cgaatgatac tttatgaaga tgattcagta caagtacaat 121 atgttgatgg ttccacattg caactttctc cctgtggctc tgaattttta tttgaaaagt 181 caccacctgt ttcagcacat cctttagaac aaccagaaag aattcgtcaa aggacacatt 241 ttgtcattag cacttacaga gagcaactac agcgagccct agattttcga aactcttcag 301 ctacttgccc ttttttatct gaaaccatca taccttctga aagaaaaaag catatcttca 361 ttgacataac agaagtgaga tggcccagtc ttgatacgga tggtaccatg atatatatgg 421 agagtggcat tgtgaagata acatctttag atggtcatgc atacctctgc ctgcccagat 481 ctcagcatga atttacagta cattttttgt gtaaagttag ccagaagtca gactcatctg 541 cagtgttgtc agaaacaaat aataaagccc caaaagataa actagttgaa aaaactggca 601 aaatctgtat acgtggaaat ttaccaggac agagactgaa gaataaagaa aatgagtttc 661 attgccagat catgaaatcc aaagaaactt taaagaagat gagttgtgta aatggaactg 721 aagggaggga agagctgcct tcgcctggta caaagcacac atgtgtatac acatgggtca 781 agcagtgctg gtctgtggct gcctgtccag aggaatggaa atatcctttg tctttagcac 841 ttcattttca taataaaatc agcaatatgt ctaaaattga tgcacatata actcagagta 901 gatttttaac ttctgatatt tcagaggaaa gaggaaaagt ggtttctgtt cttcccaggg 961 ccctgtcact cagctgtcca gtcccacacc tacacaggtg gaatttttgt gattcacttt 1021 tacagagaca atctgatgaa tattcctatc ctgaactagt gaaaatggtt tggtacaaag 1081 gtgttacata tagacttacc catcaaaata tgaactcaat agagatttat tctggggatg 1141 gatctgtttt caaatcagaa ggggcttatt ttgggaacta ttttacttat tattctattc 1201 aagaaggatc aggaaagaga gaagagaaaa cttactcagt aaataacctt cctccagata 1261 gaccgggaag tccattcact gtcggttctc taattaaaca ggcaacaaga attcttcaac 1321 attgtgtgaa gatgagactt tctttaagcc ataactatcg tatatgctgc tggaaaatgg 1381 tacctgggat aaatgatagc aatatactgc ctttggtttt gaaagagtca ctcataccca 1441 gtgtgggaag atttcttgcc tactctgatg acaaagtaca tgctatcttt ttagatggca 1501 ttactctaac cctaaattGG AATTTCAGCT CTCCTATTGA GAAGAGACAG GTAAATCAAG 1561 GTCTTAACTT AGGTTGGTGT AAGTTAACTT TTCCTGATGG ACAAGAGCAG TTAATTCAGA 1621 TTGAACACCC TGAACCATAT GAAAGATATG TGACAACAGT AACATCATGG TGCAGGAGAC 1681 TGACCCAGAC TAGTCCCAGA GAGATGCCCA CTCATTCGTC ATCTTCTGTT CTCCAAGAAA 1741 ATTGGTCTGT TGCTTCTGAA CTTGAAAAGA TACAGAAGTT TAACTTGCTA CTAGAAAATA 1801 GTGGTATCCT AAACCAGATT TCTAACAAGA AAAATGAACA ACAGTCTTTT GATCATTATA 1861 AACCAGGATC TTCAGAAACC TTGCTAGGAG AAGTTAATGA AAATAGAGTA TCAATAGCAT 1921 TGAAAAAAAC CTCTGAAATC CTTCACGATA TTGACTGTCT TCTATCAAAC TCTAAAAAGT 1981 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 2041 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 2101 TTTATATATC TTGTGGAAAG GACGACGGAC AAGGGTAATG TCTGTTTCGA CGCGTTAAGT 2161 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt