Construct: ORF TRCN0000473108
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005393.1_s317c1
- Derived from:
- ccsbBroadEn_12163
- DNA Barcode:
- CCGTTACGCATCTTTGCGTAGGCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HEATR1 (55127)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473108
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55127 | HEATR1 | HEAT repeat containing 1 | NM_018072.6 | 16.2% | 16.1% | (many diffs) |
| 2 | mouse | 217995 | Heatr1 | HEAT repeat containing 1 | NM_144835.4 | 13.8% | 15.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1113
- ORF length:
- 1047
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ttctgcgtca caggctaata tccgtctcac atctcttaaa aagacactgg 121 ctaccacact tgcaccccga gtcctgttgc ccgccatcaa aaaaacttac aagcagattg 181 agaagaactg gaagaatcac atgggtccgt ttatgagcat cttgcaagag catattgggg 241 cgatgaagaa ggaagagctc acctcccatc agtctcagct aaccgccttt ttcctggagg 301 ccctggactt ccgagcccag cactctgaga acgatctgga ggaagttgga aaaacggaaa 361 attgtatcat tgactgtcta gtagccatgg ttgtcaaact ttccgaggtc acattcaggc 421 ccctgttctt caagctgttt gattgggcta aaacagaaga tgccccaaag gacaggttgt 481 tgacatttta caacttggca gattgcattg ctgaaaagct gaaagggctt tttactctgt 541 ttgccggcca cttagtgaag ccttttgctg acaccttgga ccaggtgaac atcTCCAAAA 601 CAGATGAAGC ATTTTTTGAC TCTGAAAATG ACCCTGAAAA GTGCTGCTTG CTGTTGCAGT 661 TTATTTTGAA CTGTTTATAC AAAATCTTCC TTTTTGATAC CCAGCATTTT ATAAGTAAAG 721 AGAGAGCAGG AGCCTTGATG ATGCCTCTGG TGGATCAGCT GGAAAACAGG CTTGGGGGAG 781 AAGAGAAATT CCAGGAACGG GTGACAAAGC ACCTGATACC ATGCATCGCA CAGTTTTCAG 841 TGGCCATGGC GGATGACTCT CTTTGGAAAC CACTGAACTA CCAGATTCTG CTAAAGACGA 901 GAGACTCCTC GCCTAAGGTT CGATTTGCTG CTTTGATTAC TGTGTTAGCA CTGGCTGAAA 961 AACTAAAGGA GAATTATATT GTCTTGCTAC CAGAATCCAT TCCTTTCTTA GCAGAGTTGA 1021 TGGAAGATGA ATGTGAAGAA GTAGAACATC AGTGCCAAAA GACTATTCAG CAACTGGAAA 1081 CTGTCCTGGG AGAGCCACTC CAGAGCTATT TCTACCCAAC TTTCTTGTAC AAAGTGGTTG 1141 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1201 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACC 1261 GTTACGCATC TTTGCGTAGG CTACGCGTTA AGTCgacaat caacctctgg attacaaaat 1321 ttgtgaaaga tt