Construct: ORF TRCN0000473112
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015985.1_s317c1
- Derived from:
- ccsbBroadEn_11017
- DNA Barcode:
- AAAGCGGGCGCATTTGCAGTCACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PBX3 (5090)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473112
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5090 | PBX3 | PBX homeobox 3 | XM_006717133.2 | 100% | 100% | |
| 2 | human | 5090 | PBX3 | PBX homeobox 3 | XM_011518755.2 | 72.1% | 72.1% | 1_318del |
| 3 | human | 5090 | PBX3 | PBX homeobox 3 | NM_001134778.2 | 66.5% | 66.5% | 1_318del;617_618ins63 |
| 4 | human | 5090 | PBX3 | PBX homeobox 3 | XM_006717130.1 | 60.2% | 60.2% | 1_543del |
| 5 | human | 5090 | PBX3 | PBX homeobox 3 | NM_006195.6 | 55.6% | 55.6% | 1_543del;842_843ins63 |
| 6 | human | 5090 | PBX3 | PBX homeobox 3 | XM_017014786.1 | 44.7% | 40.7% | (many diffs) |
| 7 | human | 5090 | PBX3 | PBX homeobox 3 | XM_006717131.1 | 41.9% | 38.6% | 1_543del;1071_1072ins113;1116_1117ins136 |
| 8 | human | 5090 | PBX3 | PBX homeobox 3 | NM_001330782.2 | 37.3% | 34.1% | (many diffs) |
| 9 | human | 5090 | PBX3 | PBX homeobox 3 | NR_024122.2 | 28.5% | 1_382del;681_682ins63;1142_2598del | |
| 10 | human | 5090 | PBX3 | PBX homeobox 3 | NR_024123.2 | 24.2% | (many diffs) | |
| 11 | mouse | 18516 | Pbx3 | pre B cell leukemia homeobox 3 | XM_006497784.1 | 87.4% | 91.9% | (many diffs) |
| 12 | mouse | 18516 | Pbx3 | pre B cell leukemia homeobox 3 | XM_006497782.1 | 63% | 66.3% | (many diffs) |
| 13 | mouse | 18516 | Pbx3 | pre B cell leukemia homeobox 3 | NM_016768.2 | 52.6% | 55.3% | (many diffs) |
| 14 | mouse | 18516 | Pbx3 | pre B cell leukemia homeobox 3 | NM_001290576.1 | 35% | 34.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 888
- ORF length:
- 822
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa ccttctccga gaacagagta gaacacgtcc catttctcca aaagagattg 121 aaagaatggt gggcatcatc catcgaaaat ttagttccat tcagatgcag ctcaaacaaa 181 gcacttgtga agcagttatg attttaagat caaggttcct tgatgccaga cggaaaaggc 241 gtaacttcag taaacaggcc acagaaatct tgaatgaata tttttactca cacctcagca 301 acccctaccc cagtgaagaa gccaaagagg agctggccaa gaaatgcagc atcacagtgt 361 cacagagtct tgtaaaggat ccaaaagaaa gagggagcaa aggttctgac atccagccaa 421 ctagcgtggt atccaattgg tttggcaaca aacgaatcag gtacaagaag aacattggca 481 agtttcagga agaagccaac ctctatgctg caaagacggc cgtgacagct gcacacgcag 541 tagcagcagc tgtgcagaaC AACCAGACCA ATTCGCCCAC CACACCAAAT TCCGGTTCTT 601 CTGGTTCTTT TAACCTCCCA AATTCTGGGG ACATGTTCAT GAACATGCAG AGTCTGAATG 661 GGGATTCTTA CCAAGGGTCC CAAGTCGGAG CCAATGTGCA ATCACAGGTG GATACCCTCC 721 GTCATGTTAT CAATCAGACG GGAGGCTACA GTGATGGCCT TGGAGGAAAT TCACTGTACA 781 GTCCACATAA TTTAAATGCT AATGGAGGCT GGCAGGACGC AACAACTCCA TCTTCTGTGA 841 CTTCTCCTAC AGAAGGCCCA GGAAGTGTGC ACTCGGATAC CTCTAACTAC CCAACTTTCT 901 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 961 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1021 GTGGAAAGGA CGAAAAGCGG GCGCATTTGC AGTCACTACG CGTTAAGTCg acaatcaacc 1081 tctggattac aaaatttgtg aaagatt