Construct: ORF TRCN0000473122
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000531.1_s317c1
- Derived from:
- ccsbBroadEn_13715
- DNA Barcode:
- GGGTCAGTAGTGGATGTACCAGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LOC554223 (554223)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473122
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3135 | HLA-G | major histocompatibility co... | XM_017010817.1 | 37.4% | 30.4% | (many diffs) |
| 2 | human | 3135 | HLA-G | major histocompatibility co... | XM_017010818.1 | 37.4% | 30.4% | (many diffs) |
| 3 | human | 3135 | HLA-G | major histocompatibility co... | NM_002127.5 | 26.9% | 22.1% | (many diffs) |
| 4 | human | 3135 | HLA-G | major histocompatibility co... | XM_024446420.1 | 26.9% | 22.1% | (many diffs) |
| 5 | human | 3135 | HLA-G | major histocompatibility co... | NM_001363567.1 | 26.5% | 21.8% | (many diffs) |
| 6 | human | 3105 | HLA-A | major histocompatibility co... | NM_002116.8 | 25.1% | 21% | (many diffs) |
| 7 | human | 3105 | HLA-A | major histocompatibility co... | NM_001242758.1 | 24.9% | 20.8% | (many diffs) |
| 8 | human | 352962 | HLA-V | major histocompatibility co... | NR_132323.1 | 24.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 408
- ORF length:
- 318
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGCGCCCCG AACCCTGCTT CTGCTGCTCT 121 CGGGGGCCCT GGTCCTGACC CAGACCTGGG CAGGCTTCCA CTCCTTGAGG TATTTCCACA 181 CCACCATGTC CCGGCCCGGC CGCGCGGATC CCCGCTTCCT CTCCGTGGGC GACGTGGACG 241 ACACGCAGTG CGTGCGGCTC GACAGCGACG CCACGAGTCC CAGGATGGAG CCGGAGGGGC 301 CGGAATATTG GGAAGAGGAG ACAGGGACCG CCAAGGCCAA AGCACAGTTT TACCGAGTGA 361 ACCTGCGGAC CCTGAGCGGC TACTACAACC AGAGTGAGGC CTGTGAGTGC CCAACTTTCT 421 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 481 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 541 GTGGAAAGGA CGAGGGTCAG TAGTGGATGT ACCAGTAACG CGTTAAGTCg acaatcaacc 601 tctggattac aaaatttgtg aaagatt