Construct: ORF TRCN0000473142
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014929.1_s317c1
- Derived from:
- ccsbBroadEn_12536
- DNA Barcode:
- GGTAGCTTATCCAACCTAGGTTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SECISBP2 (79048)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473142
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79048 | SECISBP2 | SECIS binding protein 2 | NM_001354698.2 | 10.7% | 8.1% | 183_303del;374_2355del |
| 2 | human | 79048 | SECISBP2 | SECIS binding protein 2 | XM_024447666.1 | 10.6% | 8.1% | 183_303del;374_2358del |
| 3 | human | 79048 | SECISBP2 | SECIS binding protein 2 | NM_001354696.2 | 10.3% | 7.8% | 183_303del;374_2445del |
| 4 | human | 79048 | SECISBP2 | SECIS binding protein 2 | NM_001354697.2 | 10.2% | 7.8% | 183_303del;374_2448del |
| 5 | human | 79048 | SECISBP2 | SECIS binding protein 2 | XM_017015122.2 | 10.1% | 7.7% | 183_303del;374_2472del |
| 6 | human | 79048 | SECISBP2 | SECIS binding protein 2 | NM_001282688.2 | 9.8% | 7.5% | 183_303del;374_2559del |
| 7 | human | 79048 | SECISBP2 | SECIS binding protein 2 | NM_024077.5 | 9.8% | 7.4% | 183_303del;374_2562del |
| 8 | human | 79048 | SECISBP2 | SECIS binding protein 2 | XR_929843.1 | 6.9% | (many diffs) | |
| 9 | human | 79048 | SECISBP2 | SECIS binding protein 2 | XM_024447667.1 | 6.6% | 1.9% | 0_1ins98;155_2226del |
| 10 | human | 79048 | SECISBP2 | SECIS binding protein 2 | NM_001282689.2 | 6.3% | 1.8% | 0_1ins98;155_2343del |
| 11 | human | 79048 | SECISBP2 | SECIS binding protein 2 | XM_006717282.2 | 6.3% | 1.8% | 0_1ins98;155_2343del |
| 12 | human | 79048 | SECISBP2 | SECIS binding protein 2 | XR_002956807.1 | 6.3% | (many diffs) | |
| 13 | human | 79048 | SECISBP2 | SECIS binding protein 2 | XR_001746377.1 | 5.9% | (many diffs) | |
| 14 | human | 79048 | SECISBP2 | SECIS binding protein 2 | XR_929840.1 | 5.3% | (many diffs) | |
| 15 | human | 79048 | SECISBP2 | SECIS binding protein 2 | XR_001746378.1 | 3.9% | (many diffs) | |
| 16 | human | 79048 | SECISBP2 | SECIS binding protein 2 | XR_001746376.1 | 3.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 272
- ORF end:
- 524
- ORF length:
- 252
- Sequence:
-
1 CACCTCGATT AGTTCTCGAG CTTTTGGAGT ACGTCGTCTT TAGGTTGGGG GGAGGGGTTT 61 TATGCGATGG AGTTTCCCCA CACTGAGTGG GTGGAGACTG AAGTTAGGCC AGCTTGGCAC 121 TTGATGTAAT TCTCCTTGGA ATTTGCCCTT TTTGAGTTTG GATCTTGGTT CATTCTCAAG 181 CCTCAGACAG TGGTTCAAAG TTTTTTTCTT CCATTTCAGG TGTCGTGAGG CTAGCATCGA 241 TTGATCAACA AGTTTGTACA AAAAAGTTGG CATGGCGTCG GAGGGGCCGC GGGAGCCCGA 301 AAGCGAGGGC ATCAAGTTAT CAGCAGATGT CAAACCATTT GTCCCCAGAT TTGCCGGGCT 361 CAATGTGGCA TGGTTAGAGT CCTCAGAAGC ATGTGTCTTC CCCAGCTCTG CAGCCACATA 421 CTATCCGTTT GTTCAGGAAC CACCAGTGAC AGAAATGTTT ACTCAGTGCC TGGCTCCCAG 481 TATCTTTATA ACCAACCCAG TTGTTACCGA GGTTTTCAAA CAGTGCCCAA CTTTCTTGTA 541 CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA 601 GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG 661 AAAGGACGAG GTAGCTTATC CAACCTAGGT TTTACGCGTT AAGTCgacaa tcaacctctg 721 gattacaaaa tttgtgaaag att