Construct: ORF TRCN0000473152
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011915.1_s317c1
- Derived from:
- ccsbBroadEn_07627
- DNA Barcode:
- CAACACTATTGGGCCTTTGGGCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CIB2 (10518)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473152
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10518 | CIB2 | calcium and integrin bindin... | NM_006383.4 | 99.8% | 100% | 477C>T |
| 2 | human | 10518 | CIB2 | calcium and integrin bindin... | XM_005254126.3 | 86.4% | 86.6% | (many diffs) |
| 3 | human | 10518 | CIB2 | calcium and integrin bindin... | NM_001301224.2 | 84.2% | 76.2% | (many diffs) |
| 4 | human | 10518 | CIB2 | calcium and integrin bindin... | NM_001271888.2 | 76.8% | 77% | 0_1ins129;348C>T |
| 5 | human | 10518 | CIB2 | calcium and integrin bindin... | XM_006720374.2 | 76.8% | 77% | 0_1ins129;348C>T |
| 6 | human | 10518 | CIB2 | calcium and integrin bindin... | NM_001271889.2 | 73.6% | 73.7% | 50_51ins147;330C>T |
| 7 | human | 10518 | CIB2 | calcium and integrin bindin... | XM_011521161.2 | 66.2% | 66.1% | (many diffs) |
| 8 | human | 10518 | CIB2 | calcium and integrin bindin... | NR_125435.2 | 28.1% | (many diffs) | |
| 9 | human | 10518 | CIB2 | calcium and integrin bindin... | XR_001751051.1 | 26.5% | (many diffs) | |
| 10 | mouse | 56506 | Cib2 | calcium and integrin bindin... | NM_019686.5 | 90.3% | 97.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 627
- ORF length:
- 561
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gaacaagcag accatcttca ccgaagagca gctagacaac taccaggact 121 gcaccttctt caataagaag gacatcctca agctgcattc gcgattctat gagctggccc 181 ccaacctcGT CCCAATGGAC TACAGGAAGA GCCCCATCGT CCACGTGCCC ATGAGCCTCA 241 TCATCCAGAT GCCAGAGCTC CGGGAGAATC CCTTCAAAGA AAGGATCGTG GCGGCGTTTT 301 CCGAGGATGG TGAGGGGAAC CTCACTTTCA ACGACTTTGT GGACATGTTT TCCGTGCTCT 361 GCGAGTCGGC TCCCCGAGAG CTCAAGGCAA ACTATGCCTT CAAGATCTAT GACTTCAACA 421 CTGACAACTT CATCTGCAAG GAGGACCTGG AGCTGACGCT GGCCCGGCTC ACTAAGTCAG 481 AGCTGGATGA GGAGGAGGTG GTGCTTGTGT GCGACAAGGT CATTGAGGAG GCTGACTTGG 541 ATGGTGACGG CAAGCTGGGC TTTGCTGACT TCGAGGACAT GATTGCCAAG GCCCCTGACT 601 TCCTCAGCAC TTTCCACATC CGGATCTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 661 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 721 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACAACACTA 781 TTGGGCCTTT GGGCACACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 841 aagatt