Transcript: Human NR_125435.2

Homo sapiens calcium and integrin binding family member 2 (CIB2), transcript variant 5, non-coding RNA.

Source:
NCBI, updated 2019-07-11
Taxon:
Homo sapiens (human)
Gene:
CIB2 (10518)
Length:
1477
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_125435.2
NBCI Gene record:
CIB2 (10518)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_125435.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000039596 CGAGAGCTCAAGGCAAACTAT pLKO.1 518 3UTR 100% 5.625 7.875 N CIB2 n/a
2 TRCN0000332967 CGAGAGCTCAAGGCAAACTAT pLKO_005 518 3UTR 100% 5.625 7.875 N CIB2 n/a
3 TRCN0000039597 GAACCTCACTTTCAACGACTT pLKO.1 460 3UTR 100% 4.050 3.240 N CIB2 n/a
4 TRCN0000039593 GTCCTTTCCTACTCCAGAAAT pLKO.1 1037 3UTR 100% 13.200 9.240 N CIB2 n/a
5 TRCN0000332968 GTCCTTTCCTACTCCAGAAAT pLKO_005 1037 3UTR 100% 13.200 9.240 N CIB2 n/a
6 TRCN0000018334 CTGACTTCGAGGACATGATTG pLKO.1 708 3UTR 100% 10.800 7.560 N CIB2 n/a
7 TRCN0000344569 CTGACTTCGAGGACATGATTG pLKO_005 708 3UTR 100% 10.800 7.560 N CIB2 n/a
8 TRCN0000039594 GCTGACTTCGAGGACATGATT pLKO.1 707 3UTR 100% 5.625 3.938 N CIB2 n/a
9 TRCN0000010367 CAACTACCAGGACTGCACCTT pLKO.1 362 3UTR 100% 2.640 1.848 N CIB2 n/a
10 TRCN0000104786 CAAGGCAAACTATGCCTTCAA pLKO.1 526 3UTR 100% 0.495 0.347 N Cib2 n/a
11 TRCN0000010368 CCTCCTTCACAATGTGAAGCT pLKO.1 1264 3UTR 100% 0.264 0.185 N CIB2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_125435.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07627 pDONR223 100% 28.1% None (many diffs) n/a
2 ccsbBroad304_07627 pLX_304 0% 28.1% V5 (many diffs) n/a
3 TRCN0000473152 CAACACTATTGGGCCTTTGGGCAC pLX_317 77.2% 28.1% V5 (many diffs) n/a
4 ccsbBroadEn_14049 pDONR223 100% 26.9% None (many diffs) n/a
5 ccsbBroad304_14049 pLX_304 0% 26.9% V5 (many diffs) n/a
6 TRCN0000474105 ACATTCCCCCCTCAATTCTAAAAC pLX_317 61% 26.9% V5 (many diffs) n/a
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