Construct: ORF TRCN0000473165
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010368.1_s317c1
- Derived from:
- ccsbBroadEn_12303
- DNA Barcode:
- GGCGTTGGAGAATCTAACCAGGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRMT8 (56341)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473165
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56341 | PRMT8 | protein arginine methyltran... | NM_001256536.1 | 86.5% | 86.4% | 1_153del;421G>C;724C>A |
| 2 | human | 56341 | PRMT8 | protein arginine methyltran... | NM_019854.5 | 84.6% | 84.5% | 1_180del;448G>C;751C>A |
| 3 | human | 56341 | PRMT8 | protein arginine methyltran... | XM_017019645.1 | 83.3% | 79.4% | (many diffs) |
| 4 | human | 56341 | PRMT8 | protein arginine methyltran... | XM_017019644.1 | 78.2% | 78.1% | (many diffs) |
| 5 | human | 56341 | PRMT8 | protein arginine methyltran... | XM_024449059.1 | 63% | 56.3% | (many diffs) |
| 6 | human | 56341 | PRMT8 | protein arginine methyltran... | XR_001748813.2 | 42.2% | (many diffs) | |
| 7 | mouse | 381813 | Prmt8 | protein arginine N-methyltr... | XM_006506352.3 | 86.2% | 91% | (many diffs) |
| 8 | mouse | 381813 | Prmt8 | protein arginine N-methyltr... | NM_201371.2 | 79.6% | 84.2% | (many diffs) |
| 9 | mouse | 381813 | Prmt8 | protein arginine N-methyltr... | XR_001785148.1 | 33.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1068
- ORF length:
- 1002
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc caagctgctg aacccagagg agatgacctc gagagattat tacttcgact 121 cctatgccca ctttgggatc cacgaggaaa tgctgaagga tgaggtgcgg actctcactt 181 accggaactc catgtaccac aacaagcacg tgttcaagga caaagtggta ctggatgtgg 241 ggagtggtac tgggatcctt tccatgttcg ctgccaaggc aggggccaag aaggtgtttg 301 ggatcgaatg ctccagtatt tctgactact cacagaagat cattaaggcc aaccacttgg 361 acaacatcat caccatattt aagggtaaag tggaagaggt ggagctgcct gtggagaagg 421 tggacatcat catcagcgag tggatgggct actgtctgtt ctatgagtcc atgctcaaca 481 cggtgatctt tgccagggac aagtggctga aacctggagg gcttatgttt ccagaccggg 541 cagctttgta cgtggtagcg attgaagaca gacagtacaa ggacttcaaa atccactggt 601 gggagaatgt ctatggcttt gacatgaccT GCATCAGGGA CGTGGCCATG AAGGAGCCTC 661 TAGTGGACAT CGTGGATCCA AAGCAAGTGG TGACCAATGC CTGTTTGATA AAGGAGGTGG 721 ACATTTACAC AGTGAAGACG GAAGAGCTAT CGTTCACATC TGCATTCTGC CTGCAGATAC 781 AGCGCAACGA CTACGTCCAC GCCCTGGTCA CCTATTTTAA TATTGAATTT ACCAAGTGCC 841 ACAAGAAAAT GGGGTTTTCC ACAGCCCCTG ATGCTCCCTA CACCCACTGG AAGCAGACCG 901 TCTTCTACTT GGAAGATTAC CTCACTGTCC GGAGGGGGGA GGAAATCTAC GGGACCATAT 961 CCATGAAGCC AAATGCCAAA AATGTGCGAG ACCTCGATTT CACAGTAGAC TTGGATTTTA 1021 AGGGACAGCT GTGTGAAACA TCTGTATCTA ATGACTACAA AATGCGTTAC CCAACTTTCT 1081 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1141 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1201 GTGGAAAGGA CGAGGCGTTG GAGAATCTAA CCAGGCGACG CGTTAAGTCg acaatcaacc 1261 tctggattac aaaatttgtg aaagatt