Construct: ORF TRCN0000473167
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016829.1_s317c1
- Derived from:
- ccsbBroadEn_15849
- DNA Barcode:
- AATCTGCCTAGTCGGATTGATCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SIRT6 (51548)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473167
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51548 | SIRT6 | sirtuin 6 | NM_001193285.3 | 99.7% | 99.6% | 137G>A;984C>T |
| 2 | human | 51548 | SIRT6 | sirtuin 6 | NM_016539.4 | 92.2% | 92.1% | 137G>A;530_610del;1065C>T |
| 3 | human | 51548 | SIRT6 | sirtuin 6 | XM_024451539.1 | 79.4% | 71% | (many diffs) |
| 4 | human | 51548 | SIRT6 | sirtuin 6 | NM_001321061.2 | 77.9% | 78% | 0_1ins216;768C>T |
| 5 | human | 51548 | SIRT6 | sirtuin 6 | NM_001321059.2 | 75% | 74.9% | (many diffs) |
| 6 | human | 51548 | SIRT6 | sirtuin 6 | NM_001321058.2 | 72% | 72.1% | 0_1ins216;314_394del;849C>T |
| 7 | human | 51548 | SIRT6 | sirtuin 6 | NM_001321060.2 | 67% | 50% | (many diffs) |
| 8 | human | 51548 | SIRT6 | sirtuin 6 | NM_001321062.2 | 54.2% | 54.3% | 0_1ins405;125_205del;660C>T |
| 9 | human | 51548 | SIRT6 | sirtuin 6 | NM_001321063.2 | 49.2% | 33.5% | (many diffs) |
| 10 | human | 51548 | SIRT6 | sirtuin 6 | NM_001321064.1 | 46.1% | 30.8% | (many diffs) |
| 11 | human | 51548 | SIRT6 | sirtuin 6 | XM_024451540.1 | 46.1% | 30.8% | (many diffs) |
| 12 | mouse | 50721 | Sirt6 | sirtuin 6 | NM_181586.3 | 72.2% | 75.7% | (many diffs) |
| 13 | mouse | 50721 | Sirt6 | sirtuin 6 | XM_006513861.4 | 62.4% | 65.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1050
- ORF length:
- 984
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc ggtgaattac gcggcggggc tgtcgccgta cgcggacaag ggcaagtgcg 121 gcctcccgga gatcttcgac cccccggagg agctggagcg gaaggtgtgg gaactggcga 181 ggctggtctg gcagtcttcc aatgtggtgt tccacacggg tgccggcatc agcactgcct 241 ctggcatccc cgacttcagg ggtccccacg gagtctggac catggaggag cgaggtctgg 301 cccccaagtt cgacaccacc tttgagagcg cgcggcccac gcagacccac atggcgctgg 361 tgcagctgga gcgcgtgggc ctcctccgct tcctggtcag ccagaacgtg gacgggctcc 421 atgtgcgctc aggcttcccc agggacaaac tggcagagct ccacgggaac atgtttgtgg 481 aagaatgtgc caagtgtaag acgcagtacg tccgagacac agtcgtgggc accatgggcc 541 tgaaggccac gggccggctc tgcaccgtgg ctaaggcaag ggggctgcga gcctgcagga 601 acgccgacct gtccaTCACG CTGGGTACAT CGCTGCAGAT CCGGCCCAGC GGGAACCTGC 661 CGCTGGCTAC CAAGCGCCGG GGAGGCCGCC TGGTCATCGT CAACCTGCAG CCCACCAAGC 721 ACGACCGCCA TGCTGACCTC CGCATCCATG GCTACGTTGA CGAGGTCATG ACCCGGCTCA 781 TGAAGCACCT GGGGCTGGAG ATCCCCGCCT GGGACGGCCC CCGTGTGCTG GAGAGGGCGC 841 TGCCACCCCT GCCCCGCCCG CCCACCCCCA AGCTGGAGCC CAAGGAGGAA TCTCCCACCC 901 GGATCAACGG CTCTATCCCC GCCGGCCCCA AGCAGGAGCC CTGCGCCCAG CACAACGGCT 961 CAGAGCCCGC CAGCCCCAAA CGGGAGCGGC CCACCAGCCC TGCCCCCCAC AGACCCCCCA 1021 AAAGGGTGAA GGCCAAGGCG GTCCCCAGTT GCCCAACTTT CTTGTACAAA GTGGTTGATA 1081 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1141 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAATCT 1201 GCCTAGTCGG ATTGATCCCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1261 tgaaagatt