Construct: ORF TRCN0000473210
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011398.1_s317c1
- Derived from:
- ccsbBroadEn_06224
- DNA Barcode:
- TTACACAAGGATTCCACTATAGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FSHR (2492)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473210
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2492 | FSHR | follicle stimulating hormon... | NM_000145.4 | 99.9% | 99.7% | 919G>A;2039G>A |
| 2 | human | 2492 | FSHR | follicle stimulating hormon... | NM_181446.3 | 96.1% | 95.9% | 444_445ins78;841G>A;1961G>A |
| 3 | human | 2492 | FSHR | follicle stimulating hormon... | XM_011532733.2 | 95.2% | 95% | 668_769del;1021G>A;2141G>A |
| 4 | human | 2492 | FSHR | follicle stimulating hormon... | XM_011532734.2 | 78.7% | 77.4% | (many diffs) |
| 5 | human | 2492 | FSHR | follicle stimulating hormon... | XM_011532735.2 | 61.9% | 61.7% | 0_1ins792;127G>A;1247G>A |
| 6 | human | 2492 | FSHR | follicle stimulating hormon... | XM_011532736.2 | 61.9% | 61.7% | 0_1ins792;127G>A;1247G>A |
| 7 | human | 2492 | FSHR | follicle stimulating hormon... | XM_011532740.1 | 39% | 39% | 668_769del;957_957delAins1231 |
| 8 | mouse | 14309 | Fshr | follicle stimulating hormon... | NM_013523.3 | 86.3% | 87.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2154
- ORF length:
- 2085
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccctgctc ctggtctctt tgctggcatt cctgagcttg ggctcaggat 121 gtcatcatcg gatctgtcac tgctctaaca gggtttttct ctgccaagag agcaaggtga 181 cagagattcc ttctgacctc ccgaggaatg ccattgaact gaggtttgtc ctcaccaagc 241 ttcgagtcat ccaaaaaggt gcattttcag gatttgggga cctggagaaa atagagatct 301 ctcagaatga tgtcttggag gtgatagagg cagatgtgtt ctccaacctt cccaaattac 361 atgaaattag aattgaaaag gccaacaacc tgctctacat caaccctgag gccttccaga 421 accttcccaa ccttcaatat ctgttaatat ccaacacagg tattaagcac cttccagatg 481 ttcacaagat tcattctctc caaaaagttt tacttgacat tcaagataac ataaacatcc 541 acacaattga aagaaattct ttcgtggggc tgagctttga aagtgtgatt ctatggctga 601 ataagaatgg gattcaagaa atacacaact gtgcattcaa tggaacccaa ctagatgagc 661 tgaatctaag cgataataat aatttagaag aattgcctaa tgatgttttc cacggagcct 721 ctggaccagt cattctagat atttcaagaa caaggatcca ttccctgcct agctatggct 781 tagaaaatct taagaagctg agggccaggt cgacttacaa cttaaaaaag ctgcctactc 841 tggaaaagct tgtcgccctc atggaagcca gcctcaccta tcccagccat tgctgtgcct 901 ttgcaaactg gagacggcaa atctctgagc ttcatccaat ttgcaacaaa tctattttaa 961 ggcaagaagt tgattatatg actcagacta ggggtcagag atcctctctg gcagaagaca 1021 atgagtccag ctacagcaga ggatttgaca tgacgtacac tgagtttgac tatgacttat 1081 gcaatgaagt ggttgacgtg acctgctccc ctaagccaga tgcattcaac ccatgtgaag 1141 atatcatggg gtacaacatc ctcagagtcc tgatatggtt tatcagcatc ctggccatca 1201 ctgggaacat catagtgcta gtgatcctaa ctaccagcca atataaactc acagtcccca 1261 ggttccttat gtgcaacctg gcctttgctg atctctgcat tggaatctac ctgctgctca 1321 ttgcatcagt tgatatccat accaagagcc aatatcacaa ctatgccatt gactggcaaa 1381 ctggggcagg ctgtgatgct gctggctttt tcactgtctt tgccagtgag ctgtcagtct 1441 acactctgac agctatcacc ttggaaagat ggcataccat cacgcatgcc atgcagctgg 1501 actgcaaggt gcagctccgc catgctgcca gtgtcatggt gatgggctgg atttttgctt 1561 ttgcagctgc cctctttccc atctttggca tcagcagcta catgaaggtg agcatctgcc 1621 tgcccatgga tattgacagc cctttgtcac agctgtatgt catgtccctc cttgtgctca 1681 atgtcctggc ctttgtggtc atctgtggct gctatatcca catctacctc acagtgcgga 1741 accccaacat cgtgtcctcc tctagtgaca ccaggatcgc caagcgcatg gccatgctca 1801 tcttcactga cttcctctgc atggcaccca tttctttctt tgccatttct gccTCCCTCA 1861 AGGTGCCCCT CATCACTGTG TCCAAAGCAA AGATTCTGCT GGTTCTGTTT CACCCCATCA 1921 ACTCCTGTGC CAACCCCTTC CTCTATGCCA TCTTTACCAA AAACTTTCGC AGAGATTTCT 1981 TCATTCTGCT GAGCAAGTGT GGCTGCTATG AAATGCAAGC CCAAATTTAT AGGACAGAAA 2041 CTTCATCCAC TGTCCACAAC ACCCATCCAA GGAATGGCCA CTGCTCTTCA GCTCCCAGAG 2101 TCACCAATGG TTCCACTTAC ATACTTGTCC CTCTAAGTCA TTTAGCCCAA AACTTGCCAA 2161 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 2221 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 2281 TATCTTGTGG AAAGGACGAT TACACAAGGA TTCCACTATA GCCACGCGTT AAGTCgacaa 2341 tcaacctctg gattacaaaa tttgtgaaag att