Construct: ORF TRCN0000473212
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002752.1_s317c1
- Derived from:
- ccsbBroadEn_10860
- DNA Barcode:
- ACCATGTACTTTATCTCACGGACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GRIA3 (2892)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473212
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2892 | GRIA3 | glutamate ionotropic recept... | NM_000828.4 | 91.4% | 91% | (many diffs) |
| 2 | human | 2892 | GRIA3 | glutamate ionotropic recept... | NM_007325.5 | 90.2% | 89.9% | (many diffs) |
| 3 | human | 2892 | GRIA3 | glutamate ionotropic recept... | NM_001256743.2 | 15.2% | 10.9% | (many diffs) |
| 4 | mouse | 53623 | Gria3 | glutamate receptor, ionotro... | NM_001290451.1 | 86% | 89.9% | (many diffs) |
| 5 | mouse | 53623 | Gria3 | glutamate receptor, ionotro... | XM_011251015.1 | 86% | 89.8% | (many diffs) |
| 6 | mouse | 53623 | Gria3 | glutamate receptor, ionotro... | XM_017318546.1 | 86% | 89.8% | (many diffs) |
| 7 | mouse | 53623 | Gria3 | glutamate receptor, ionotro... | NM_001281929.1 | 84.8% | 88.9% | (many diffs) |
| 8 | mouse | 53623 | Gria3 | glutamate receptor, ionotro... | NM_016886.4 | 84.8% | 88.9% | (many diffs) |
| 9 | mouse | 53623 | Gria3 | glutamate receptor, ionotro... | XM_006541520.3 | 84.8% | 88.8% | (many diffs) |
| 10 | mouse | 53623 | Gria3 | glutamate receptor, ionotro... | XM_017318545.1 | 84.8% | 88.8% | (many diffs) |
| 11 | mouse | 53623 | Gria3 | glutamate receptor, ionotro... | XM_017318547.1 | 80.7% | 82.4% | (many diffs) |
| 12 | mouse | 53623 | Gria3 | glutamate receptor, ionotro... | NR_104053.1 | 43.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2541
- ORF length:
- 2472
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccaggcag aagaaaatgg ggcaaagcgt gctccgggcg gtcttctttt 121 tagtcctggg gcttttgggt cattctcacg gaggattccc caacaccatc agcataggtg 181 gacttttcat gagaaacaca gtgcaggagc acagcgcttt ccgctttgcc gtgcagttat 241 acaacaccaa ccagaacacc accgagaagc ccttccattt gaattaccac gtagatcact 301 tggattcctc caatagtttt tccgtgacaa atgctttctg ctcccagttc tcgagagggg 361 tgtatgccat ctttggattc tatgaccaga tgtcaatgaa caccctgacc tccttctgtg 421 gggccctgca cacatccttt gttacgccta gcttccccac tgacgcagat gtgcagtttg 481 tcatccagat gcgcccagcc ttgaagggcg ctattctgag tcttctgggt cattacaagt 541 gggagaagtt tgtgtacctc tatgacacag aacgaggatt ttccatcctc caagcgatta 601 tggaagcagc agtgcaaaac aactggcaag taacagcaag gtctgtggga aacataaagg 661 acgtccaaga attcaggcgc atcattgaag aaatggacag gaggcaggaa aagcgatact 721 tgattgactg cgaagtcgaa aggattaaca caattttgga acaggttgtg atcctaggga 781 aacactcaag aggttatcac tacatgctcg ctaacctggg ttttactgat attttactgg 841 aaagagtcat gcatggggga gccaacatta caggtttcca gattgtcaac aatgaaaacc 901 ctatggttca gcagttcata cagcgctggg tgaggctgga tgaaagggaa ttccctgaag 961 ccaagaatgc accactaaag tatacatctg cattgacaca cgacgcaata ctggtcatag 1021 cagaagcttt ccgctacctg aggaggcagc gagtagatgt gtcccggaga ggaagtgctg 1081 gagactgctt agcaaatcct gctgtgccct ggagtcaagg aattgatatt gagagagctc 1141 tgaaaatggt gcaagtacaa ggaatgactg gaaatattca atttgacact tatggacgta 1201 ggacaaatta taccatcgat gtgtatgaaa tgaaagtcag tggctctcga aaagctggct 1261 actggaacga gtatgaaagg tttgtgcctt tctcagatca gcaaatcagc aatgacagtg 1321 catcctcaga gaatcggacc atagtagtga ctaccattct ggaatcacca tatgtaatgt 1381 acaagaagaa ccatgagcaa ctggaaggaa atgaacgata tgaaggctat tgtgtagacc 1441 tagcctatga aatagccaaa catgtaagga tcaaatacaa attgtccatc gttggtgacg 1501 ggaaatatgg tgcaagggat ccagagacta aaatatggaa cggcatggtt ggggaacttg 1561 tctatgggag agctgatata gctgttgctc cactcactat aacattggtc cgtgaagaag 1621 tcatagattt ttcaaagcca ttcatgagcc tgggcatctc catcatgata aagaagcctc 1681 agaaatcaaa accaggcgta ttctcatttc tggatcccct ggcttatgaa atctggatgt 1741 gcattgtctt tgcttacatt ggagtcagcg tagttctttt cctagtcagc aggttcagtc 1801 cttatgaatg gcacttggaa gacaacaatg aagaacctcg tgacccacaa agtcctcctg 1861 atcctccaaa tgaatttgga atatttaaca gtctttggtt ttccttgggt gcctttatgc 1921 agcaaggatg tgatatttct ccaagatcac tctccgggcg cattgttgga ggggtttggt 1981 ggttcttcac cctgatcata atttcttcct atactgccaa tctcgctgct ttcctgactg 2041 tggagaggat ggtttctccc atagagagtg ctgaagactt agctaaacag actgaaattg 2101 catatgggac cctggacTCC GGTTCAACAA AAGAATTTTT CAGAAGATCC AAAATTGCTG 2161 TGTACGAGAA AATGTGGTCT TACATGAAAT CAGCGGAGCC ATCTGTGTTT ACCAAAACAA 2221 CAGCAGACGG AGTGGCCCGA GTGCGAAAGT CCAAGGGAAA GTTCGCCTTC CTGCTGGAGT 2281 CAACCATGAA TGAGTACATT GAGCAGAGAA AACCATGTGA TACGATGAAA GTTGGTGGAA 2341 ATCTGGATTC CAAAGGCTAT GGTGTGGCAA CCCCTAAAGG CTCAGCATTA GGAAATGCTG 2401 TTAACCTGGC AGTATTAAAA CTGAATGAGC AAGGCCTCTT GGACAAATTG AAAAACAAAT 2461 GGTGGTACGA CAAAGGAGAG TGCGGCAGCG GGGGCGGTGA CTCCAAGAAC GCCTGTAAAC 2521 CTTGCAGTAT TGAAACTCAG TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 2581 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 2641 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAACCA TGTACTTTAT 2701 CTCACGGACA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt