Construct: ORF TRCN0000473222
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017888.1_s317c1
- Derived from:
- ccsbBroadEn_12560
- DNA Barcode:
- GGTTGCCATGTTTACTTTTCCAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DCAF10 (79269)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473222
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79269 | DCAF10 | DDB1 and CUL4 associated fa... | XM_017015129.1 | 100% | 100% | |
| 2 | human | 79269 | DCAF10 | DDB1 and CUL4 associated fa... | NM_001286810.1 | 66.8% | 66.8% | 1_384del |
| 3 | human | 79269 | DCAF10 | DDB1 and CUL4 associated fa... | XM_005251578.4 | 52.3% | 52.3% | 1_705del |
| 4 | human | 79269 | DCAF10 | DDB1 and CUL4 associated fa... | NM_024345.5 | 46.1% | 46.1% | 1_903del |
| 5 | human | 79269 | DCAF10 | DDB1 and CUL4 associated fa... | XM_017015128.1 | 44.8% | 44.8% | 1_705del;854_855ins111 |
| 6 | human | 79269 | DCAF10 | DDB1 and CUL4 associated fa... | XM_005251577.4 | 39.5% | 39.5% | 1_903del;1052_1053ins111 |
| 7 | human | 79269 | DCAF10 | DDB1 and CUL4 associated fa... | XR_929331.2 | 9.5% | 1_1005del;1411_1583del;1953_8111del | |
| 8 | human | 79269 | DCAF10 | DDB1 and CUL4 associated fa... | XR_002956808.1 | 9% | 1_1005del;1411_2017del;2387_8545del | |
| 9 | human | 79269 | DCAF10 | DDB1 and CUL4 associated fa... | XR_002956810.1 | 7.9% | (many diffs) | |
| 10 | human | 79269 | DCAF10 | DDB1 and CUL4 associated fa... | XR_002956809.1 | 7.7% | (many diffs) | |
| 11 | mouse | 242418 | Dcaf10 | DDB1 and CUL4 associated fa... | XM_006537912.3 | 47.3% | 49.2% | (many diffs) |
| 12 | mouse | 242418 | Dcaf10 | DDB1 and CUL4 associated fa... | NM_153167.2 | 41.8% | 43.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 840
- ORF length:
- 774
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg aatgaggtta acaccagatt gttccaaaat gttgatttca acgtcctctg 121 gatatctctt aattttgcat gaccttgact taactaagtc tttagaagta ggcagctatc 181 ccattttaag agcaagaaga actacttcaa gttcagattt gaccacttca tcaagttcat 241 ctggtcctag agtttctggc tcaccttgtc atcatagtga ttctaattct tctgagaaac 301 acatgtcacg agcctctcaa agagaaggag tttcaccacg aaatagtctt gaagtcgtaa 361 ccccTGAAGT TCTTGGTGAG AGTGACCACG GAAACTGCAT CACGTCCTTA CAGCTGCACC 421 CAAAAGGCTG GGCCACTCTT CTTCGGTGCT CAAGCAACAG TGATGATGAG GAGTGTACTT 481 GTGTCTATGA ATTCCAAGAA GGAGCTCCAG TGCGACCTGT CTCACCTCGC TGTTCTCTAC 541 GACTGACTCA TTACATTGAA GAAGCCAATG TAGGCAGGGG TTACATCAAA GAACTTTGCT 601 TCAGCCCCGA TGGACGAATG ATTTCTTCCC CACATGGCTA TGGGATTCGC TTGTTGGGAT 661 TTGACAAACA GTGCAGTGAA CTTGTTGACT GCTTGCCCAA AGAAGCCAGT CCCCTGCGGG 721 TGATCCGTTC TCTGTACTCT CATAATGATG TGGTACTGAC AACAAAGTTC TCTCCAACAC 781 ATTGTCAGAT TGCCTCAGGG TGCCTTAGTG GACGGGTTTC TTTGTATCAG CCAAAGTTTT 841 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 901 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 961 TTTATATATC TTGTGGAAAG GACGAGGTTG CCATGTTTAC TTTTCCAAGA CGCGTTAAGT 1021 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt