Construct: ORF TRCN0000473226
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001519.1_s317c1
- Derived from:
- ccsbBroadEn_15800
- DNA Barcode:
- AATGTATGCTGCCCCACGAAGGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C11orf54 (28970)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473226
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 28970 | C11orf54 | chromosome 11 open reading ... | NM_001369406.2 | 99.8% | 100% | 303A>G |
| 2 | human | 28970 | C11orf54 | chromosome 11 open reading ... | NM_001286071.1 | 71.3% | 60.4% | (many diffs) |
| 3 | human | 28970 | C11orf54 | chromosome 11 open reading ... | NM_001351992.2 | 71.3% | 60.4% | (many diffs) |
| 4 | human | 28970 | C11orf54 | chromosome 11 open reading ... | XM_017017617.1 | 71.3% | 60.4% | (many diffs) |
| 5 | human | 28970 | C11orf54 | chromosome 11 open reading ... | NM_001286070.1 | 68.8% | 68.9% | 1_276del;579A>G |
| 6 | human | 28970 | C11orf54 | chromosome 11 open reading ... | NM_001351990.2 | 68.8% | 68.9% | 1_276del;579A>G |
| 7 | human | 28970 | C11orf54 | chromosome 11 open reading ... | NM_001286067.2 | 64.6% | 64.7% | 1_333del;636A>G |
| 8 | human | 28970 | C11orf54 | chromosome 11 open reading ... | NM_001286068.2 | 64.6% | 64.7% | 1_333del;636A>G |
| 9 | human | 28970 | C11orf54 | chromosome 11 open reading ... | NM_001286069.2 | 64.6% | 64.7% | 1_333del;636A>G |
| 10 | human | 28970 | C11orf54 | chromosome 11 open reading ... | NM_001351985.2 | 64.6% | 64.7% | 1_333del;636A>G |
| 11 | human | 28970 | C11orf54 | chromosome 11 open reading ... | NM_001351986.2 | 64.6% | 64.7% | 1_333del;636A>G |
| 12 | human | 28970 | C11orf54 | chromosome 11 open reading ... | NM_001351987.2 | 64.6% | 64.7% | 1_333del;636A>G |
| 13 | human | 28970 | C11orf54 | chromosome 11 open reading ... | NM_001351988.2 | 64.6% | 64.7% | 1_333del;636A>G |
| 14 | human | 28970 | C11orf54 | chromosome 11 open reading ... | NM_001351989.2 | 64.6% | 64.7% | 1_333del;636A>G |
| 15 | human | 28970 | C11orf54 | chromosome 11 open reading ... | XM_011542781.2 | 64.6% | 64.7% | 1_333del;636A>G |
| 16 | human | 28970 | C11orf54 | chromosome 11 open reading ... | XM_011542782.3 | 64.6% | 64.7% | 1_333del;636A>G |
| 17 | human | 28970 | C11orf54 | chromosome 11 open reading ... | NM_001351993.2 | 52% | 52% | 1_276del;449_450ins150 |
| 18 | human | 28970 | C11orf54 | chromosome 11 open reading ... | NM_001351991.2 | 48.8% | 48.8% | 1_333del;506_507ins150 |
| 19 | human | 28970 | C11orf54 | chromosome 11 open reading ... | NM_014039.4 | 48.8% | 48.8% | 1_333del;506_507ins150 |
| 20 | mouse | 70984 | 4931406C07Rik | RIKEN cDNA 4931406C07 gene | XM_030244585.1 | 84.3% | 86.2% | (many diffs) |
| 21 | mouse | 70984 | 4931406C07Rik | RIKEN cDNA 4931406C07 gene | NM_001359258.1 | 60.9% | 62.4% | (many diffs) |
| 22 | mouse | 70984 | 4931406C07Rik | RIKEN cDNA 4931406C07 gene | NM_001199484.1 | 54.6% | 55.8% | (many diffs) |
| 23 | mouse | 70984 | 4931406C07Rik | RIKEN cDNA 4931406C07 gene | NM_001199485.1 | 54.6% | 55.8% | (many diffs) |
| 24 | mouse | 70984 | 4931406C07Rik | RIKEN cDNA 4931406C07 gene | NM_133732.3 | 54.6% | 55.8% | (many diffs) |
| 25 | mouse | 70984 | 4931406C07Rik | RIKEN cDNA 4931406C07 gene | XM_006510596.2 | 54.6% | 55.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 678
- ORF length:
- 612
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc agttattcag acagaaagtg aacacaagcc tcctgtaaat ggaagttact 121 ttgcccatgt gaaccctgca gatggagggt gcctactGGA GAAATACAGT GAGAAATGTC 181 ATGATTTTCA GTGTGCATTA CTGGCTAATC TTTTTGCCAG TGAAGGCCAA CCTGGCAAGG 241 TAATTGAGGT GAAAGCCAAA AGAAGAACTG GACCACTTAA CTTTGTGACT TGTATGAGAG 301 AGACCCTGGA AAAACATTAT GGAAATAAGC CTATAGGAAT GGGAGGTACT TTCATAATTC 361 AGAAGGGGAA AGTGAAGTCT CACATTATGC CTGCAGAATT TTCTTCCTGC CCCTTGAACT 421 CTGATGAAGA AGTGAATAAA TGGTTGCATT TTTATGAAAT GAAAGCTCCT TTGGTTTGTC 481 TACCAGTTTT TGTCTCCAGA GACCCAGGGT TTGATTTGCG ACTGGAGCAC ACTCATTTTT 541 TTAGTCGTCA TGGAGAAGGT GGACACTACC ATTATGACAC TACTCCAGAT ATAGTGGAAT 601 ATCTTGGATA CTTCTTACCT GCAGAGTTTC TCTATCGCAT TGATCAACCA AAAGAGACGC 661 ATTCAATTGG GCGAGATTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 721 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 781 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAAATGTAT GCTGCCCCAC 841 GAAGGTGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt