Transcript: Mouse XM_006510596.2

PREDICTED: Mus musculus RIKEN cDNA 4931406C07 gene (4931406C07Rik), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Mus musculus (mouse)
Gene:
4931406C07Rik (70984)
Length:
2618
CDS:
190..1137

Additional Resources:

NCBI RefSeq record:
XM_006510596.2
NBCI Gene record:
4931406C07Rik (70984)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006510596.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000197489 CCTGCTATCTAGGAATAACTT pLKO.1 1569 3UTR 100% 5.625 7.875 N 4931406C07Rik n/a
2 TRCN0000176509 CCTCTTATTATGTGGTGTCTT pLKO.1 1925 3UTR 100% 4.950 3.960 N 4931406C07Rik n/a
3 TRCN0000177251 GCTACACTTCTATGAGATGAA pLKO.1 900 CDS 100% 4.950 3.960 N 4931406C07Rik n/a
4 TRCN0000277257 GCTACACTTCTATGAGATGAA pLKO_005 900 CDS 100% 4.950 3.960 N 4931406C07Rik n/a
5 TRCN0000285938 GACACTCATATTACCATATTA pLKO_005 1394 3UTR 100% 15.000 10.500 N 4931406C07Rik n/a
6 TRCN0000277302 TCGGACGAAGCTGTCAATAAA pLKO_005 877 CDS 100% 15.000 10.500 N 4931406C07Rik n/a
7 TRCN0000167174 CCTTACTTATTGCCTCTTGTA pLKO.1 385 CDS 100% 4.950 3.465 N C11orf54 n/a
8 TRCN0000197953 GCTAATTGAGATGCTCACTTT pLKO.1 1458 3UTR 100% 4.950 3.465 N 4931406C07Rik n/a
9 TRCN0000178173 GTGCCTTACTTATTGCCTCTT pLKO.1 382 CDS 100% 4.050 2.835 N 4931406C07Rik n/a
10 TRCN0000182341 GCAAGTGAACACAACCAACCT pLKO.1 541 CDS 100% 2.640 1.848 N 4931406C07Rik n/a
11 TRCN0000277303 GGAAGTTACTTTGCCCATAAA pLKO_005 568 CDS 100% 13.200 7.920 N 4931406C07Rik n/a
12 TRCN0000177094 CAGATTTAACAAAGGAGCCAT pLKO.1 305 CDS 100% 2.640 1.584 N 4931406C07Rik n/a
13 TRCN0000285941 CAGATTTAACAAAGGAGCCAT pLKO_005 305 CDS 100% 2.640 1.584 N 4931406C07Rik n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006510596.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03048 pDONR223 100% 73.1% 74.9% None (many diffs) n/a
2 ccsbBroad304_03048 pLX_304 0% 73.1% 74.9% V5 (many diffs) n/a
3 TRCN0000473941 AACTTGGAATTAACTTCTTAAGCA pLX_317 47.2% 73.1% 74.9% V5 (many diffs) n/a
4 ccsbBroadEn_15800 pDONR223 0% 54.6% 55.8% None (many diffs) n/a
5 ccsbBroad304_15800 pLX_304 0% 54.6% 55.8% V5 (many diffs) n/a
6 TRCN0000473226 AATGTATGCTGCCCCACGAAGGTG pLX_317 84% 54.6% 55.8% V5 (many diffs) n/a
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